Recombinant
RabMAb

Recombinant Anti-TEAD4/TEF-1 antibody [EPR15629] (ab197589)

Overview

  • Product name

    Anti-TEAD4/TEF-1 antibody [EPR15629]
    See all TEAD4/TEF-1 primary antibodies
  • Description

    Rabbit monoclonal [EPR15629] to TEAD4/TEF-1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Recombinant fragment within Human TEAD4/TEF-1 aa 150-300. The exact sequence is proprietary. Immunogen sequence from TEAD4/TEF-1.
    Database link: Q15561

  • Positive control

    • HeLa and SW480 whole cell lysates; Human TEAD1, TEAD2, TEAD3 and TEAD4/TEF-1 recombinant proteins (GST-Tag).
  • General notes

     

     

     This product was previously labelled as TEAD4

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol, 0.05% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR15629
  • Isotype

    IgG

Applications

Our Abpromise guarantee covers the use of ab197589 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Detects a band of approximately 54 kDa (predicted molecular weight: 48 kDa).

Target

  • Cellular localization

    Nuclear
  • Database links

  • Alternative names

    • DTEF 1 antibody
    • NTEF 1 antibody
    • Protein GT-IIC antibody
    • SV40 transcriptional enhancer factor antibody
    • TCF 13 antibody
    • TCF13 antibody
    • TEA domain family member 1 antibody
    • TEA domain family member 2 antibody
    • TEA domain family member 3 antibody
    • TEA domain family member 4 antibody
    • TEAD 1 antibody
    • TEAD 2 antibody
    • TEAD 3 antibody
    • TEAD 4 antibody
    • TEAD2 antibody
    • TEAD3 antibody
    • TEAD4 antibody
    • TEAD5 antibody
    • TEF1 antibody
    • TEF4 antibody
    • TEF5 antibody
    • Transcription factor 13 antibody
    • Transcription factor 13 like 1 antibody
    • Transcription factor RTEF 1 antibody
    • Transcriptional enhancer factor TEF 1 antibody
    • Transcriptional enhancer factor TEF 3 antibody
    • Transcriptional enhancer factor TEF 4 antibody
    • Transcriptional enhancer factor TEF 5 antibody
    see all

Images

  • All lanes : Anti-TEAD4/TEF-1 antibody [EPR15629] (ab197589) at 1/1000 dilution

    Lane 1 : HeLa (Human epithelial cells from cervix adenocarcinoma) whole cell lysate
    Lane 2 : SW480 (Human colorectal adenocarcinoma cell line) whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 48 kDa
    Observed band size: 54 kDa
    why is the actual band size different from the predicted?


    Exposure time: 1 minute


    Blocking/Dilution buffer: 5% NFDM/TBST.

  • All lanes : Anti-TEAD4/TEF-1 antibody [EPR15629] (ab197589) at 1/5000 dilution

    Lane 1 : Human TEAD4/TEF-1 recombinant protein (GST-Tag) aa165-280
    Lane 2 : Human TEAD4/TEF-1 recombinant protein (GST-Tag) aa161-280
    Lane 3 : Human TEAD2 recombinant protein (GST-Tag) aa161-290
    Lane 4 : Human TEAD3 recombinant protein (GST-Tag) aa161-290

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilution (Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated)

    Predicted band size: 48 kDa
    Observed band size: 39 kDa why is the actual band size different from the predicted?


    Exposure time: 5 seconds


    5% NFDM/TBST: Blocking and diluting buffer.

References

This product has been referenced in:

  • Lin J  et al. Neuropilin 1 (NRP1) is a novel tumor marker in hepatocellular carcinoma. Clin Chim Acta 485:158-165 (2018). Read more (PubMed: 29966621) »
  • Unterer B  et al. IFN-?-response mediator GBP-1 represses human cell proliferation by inhibiting the Hippo signaling transcription factor TEAD. Biochem J 475:2955-2967 (2018). Read more (PubMed: 30120107) »
See all 2 Publications for this product

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