Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR15629] to TEAD4/TEF-1 - BSA and Azide free
- Suitable for: WB
- Reacts with: Human
Product nameAnti-TEAD4/TEF-1 antibody [EPR15629] - BSA and Azide free
See all TEAD4/TEF-1 primary antibodies
DescriptionRabbit monoclonal [EPR15629] to TEAD4/TEF-1 - BSA and Azide free
Tested applicationsSuitable for: WBmore details
Species reactivityReacts with: Human
Recombinant fragment within Human TEAD4/TEF-1 aa 150-300. The exact sequence is proprietary. Immunogen sequence from TEAD4.
Database link: Q15561
- WB: HeLa and SW480 whole cell lysates.
ab240976 is the carrier-free version of ab197589 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Ab240976 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
This product was previously labelled as TEAD4
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Storage instructionsShipped at 4°C. Store at +4°C. Do Not Freeze.
Storage bufferpH: 7.2
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab240976 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use at an assay dependent concentration. Detects a band of approximately 54 kDa (predicted molecular weight: 48 kDa).|
- DTEF 1 antibody
- NTEF 1 antibody
- Protein GT-IIC antibody
All lanes : Anti-TEAD4/TEF-1 antibody [EPR15629] (ab197589) at 1/1000 dilution
Lane 1 : HeLa (Human epithelial cells from cervix adenocarcinoma) whole cell lysate
Lane 2 : SW480 (Human colorectal adenocarcinoma cell line) whole cell lysate
Lysates/proteins at 20 µg per lane.
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 48 kDa
Observed band size: 54 kDa why is the actual band size different from the predicted?
Exposure time: 1 minute
Blocking/Dilution buffer: 5% NFDM/TBST.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab197589).
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab240976 has not yet been referenced specifically in any publications.