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Our Abpromise guarantee covers the use of ab190222 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000. Predicted molecular weight: 115 kDa.|
|IHC-P||Use a concentration of 5 µg/ml.|
|Flow Cyt||1/10 - 1/50.
ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.
|ICC/IF||1/10 - 1/50.|
Immunohistochemical analysis of formalin fixed paraffin embedded Human prostate tissue labeling Teashirt homolog 2 with ab190222 at 5 µg/ml.
Immunohistochemical analysis of formalin fixed, paraffin embedded Human brain tissue labeling Teashirt homolog 2 with ab190222 at 5 µg/ml, followed by peroxidase conjugation of the secondary antibody and DAB staining.
Immunocytochemical analysis of U251 cells labeling Teashirt homolog 2 with ab190222 at 1/10 dilution. Cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with ab190222 (1:25, 1 h at RT) followed by Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) (1:400, 50 min at RT). Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml, 1 h at RT). Nuclei were counterstained with DAPI (blue) (10 ug/ml, 10 min).
Flow cytometric analysis of CEM cells labeling Teashirt homolog 2 with ab190222 at 1/10 dilution (right histogram) compared to negative control (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
ab190222 has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"