Overview

  • Product name

  • Description

    Rabbit polyclonal to TECPR1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-P, ICC/IFmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Recombinant fragment corresponding to Human TECPR1 aa 199-487.
    Database link: Q7Z6L1

  • Positive control

    • IHC-P: Human heart tissue. ICC/IF: A-375 cells.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.40
    Preservative: 0.03% Proclin
    Constituents: 50% Glycerol, PBS
  • Concentration information loading...
  • Purity

    Protein G purified
  • Purification notes

    Purity >95%.
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab229983 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/20 - 1/200.
ICC/IF 1/50 - 1/200.

Target

  • Function

    Tethering factor involved in autophagy. Involved in autophagosome maturation by promoting the autophagosome fusion with lysosomes: acts by associating with both the ATG5-ATG12 conjugate and phosphatidylinositol-3-phosphate (PtdIns(3)P) present at the surface of autophagosomes. Also involved in selective autophagy against bacterial pathogens, by being required for phagophore/preautophagosomal structure biogenesis and maturation.
  • Sequence similarities

    Belongs to the TECPR1 family.
    Contains 1 PH domain.
    Contains 9 TECPR repeats.
  • Domain

    The PH domain mediates the binding to phosphatidylinositol-3-phosphate (PtdIns(3)P). While full-length protein is unable to bind PtdIns(3)P in vitro, it is assumed that the binding to the ATG5-ATG12 conjugate exposes the PH domain, allowing the association with PtdIns(3)P (PubMed:22342342).
  • Cellular localization

    Cytoplasmic vesicle > autophagosome membrane. Lysosome membrane. Localizes to Lysosome membranes, and binds PtdIns(3)P at the surface of autophagosome. Localizes to autolysosomes, a vesicle formed by the fusion between autophagosomes and lysosomes.
  • Information by UniProt
  • Database links

  • Alternative names

    • 2210010N04Rik antibody
    • DKFZP434B0335 antibody
    • FLJ23419 antibody
    • FLJ90593 antibody
    • KIAA1358 antibody
    • MGC124730 antibody
    • mKIAA1358 antibody
    • RGD1306873 antibody
    • TCPR1_HUMAN antibody
    • Tecpr1 antibody
    • tectonin beta propeller repeat containing 1 antibody
    • Tectonin beta-propeller repeat-containing protein 1 antibody
    see all

Images

  • Paraffin-embedded human heart tissue stained for TECPR1 using ab229983 at 1/100 dilution in immunohistochemical analysis.

  • A-375 (Human malignant melanoma cell line) cells stained for TECPR1 (green) using ab229983 at 1/100 dilution in ICC/IF, followed by Alexa Fluor 488® conjugated Goat Anti-Rabbit IgG (H+L).

References

ab229983 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab229983.
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