Recombinant
RabMAb

Recombinant Anti-TEF1/TEAD-1 antibody [EPR3967(2)] - BSA and Azide free (ab219647)

Overview

  • Product name

    Anti-TEF1/TEAD-1 antibody [EPR3967(2)] - BSA and Azide free
    See all TEF1/TEAD-1 primary antibodies
  • Description

    Rabbit monoclonal [EPR3967(2)] to TEF1/TEAD-1 - BSA and Azide free
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-P, IP, WBmore details
    Unsuitable for: Flow Cyt or ICC
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human TEF1/TEAD-1 aa 200-300. The exact sequence is proprietary.
    Database link: P28347

  • General notes

    Ab219647 is the carrier-free version of ab133533. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab219647 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

See IHC antigen retrieval protocols.

IP Use at an assay dependent concentration.
WB Use at an assay dependent concentration. Predicted molecular weight: 52 kDa.
  • Application notes
    Is unsuitable for Flow Cyt or ICC.
  • Target

    • Function

      Transcription factor which plays a key role in the Hippo signaling pathway, a pathway involved in organ size control and tumor suppression by restricting proliferation and promoting apoptosis. The core of this pathway is composed of a kinase cascade wherein MST1/MST2, in complex with its regulatory protein SAV1, phosphorylates and activates LATS1/2 in complex with its regulatory protein MOB1, which in turn phosphorylates and inactivates YAP1 oncoprotein and WWTR1/TAZ. Acts by mediating gene expression of YAP1 and WWTR1/TAZ, thereby regulating cell proliferation, migration and epithelial mesenchymal transition (EMT) induction. Binds specifically and cooperatively to the SPH and GT-IIC 'enhansons' (5'-GTGGAATGT-3') and activates transcription in vivo in a cell-specific manner. The activation function appears to be mediated by a limiting cell-specific transcriptional intermediary factor (TIF). Involved in cardiac development. Binds to the M-CAT motif.
    • Tissue specificity

      Preferentially expressed in skeletal muscle. Lower levels in pancreas, placenta, and heart.
    • Involvement in disease

      Defects in TEAD1 are the cause of Sveinsson chorioretinal atrophy (SCRA) [MIM:108985]; also known as atrophia areata (AA) or helicoidal peripapillary chorioretinal degeneration (HPCD). SCRA is characterized by symmetrical lesions radiating from the optic disk involving the retina and the choroid.
    • Sequence similarities

      Contains 1 TEA DNA-binding domain.
    • Cellular localization

      Nucleus.
    • Information by UniProt
    • Database links

    • Alternative names

      • AA antibody
      • Atrophia areata peripapillary chorioretinal degeneration antibody
      • NTEF 1 antibody
      • NTEF-1 antibody
      • NTEF1 antibody
      • Protein GT IIC antibody
      • Protein GT-IIC antibody
      • REF 1 antibody
      • REF1 antibody
      • SV40 transcriptional enhancer factor antibody
      • TCF 13 antibody
      • TCF-13 antibody
      • TCF13 antibody
      • TEA domain family member antibody
      • TEA domain family member 1 (SV40 transcriptional enhancer factor) antibody
      • TEA domain family member 1 antibody
      • TEAD 1 antibody
      • TEAD 1 protein antibody
      • TEAD-1 antibody
      • TEAD1 antibody
      • TEAD1 protein antibody
      • TEAD1_HUMAN antibody
      • TEF 1 antibody
      • TEF1 antibody
      • Transcription factor 13 (SV40 transcriptional enhancer factor) antibody
      • Transcription factor 13 antibody
      • Transcriptional enhancer factor 1 antibody
      • Transcriptional enhancer factor TEF-1 antibody
      • Transcriptional enhancer factor TEF1 antibody
      see all

    Images

    • ab133533 (purified) at 1:80 dilution (2ug) immunoprecipitating TEF1/TEAD-1 in 293 (Human embryonic kidney epithelial cell) whole cell lysate.
      Lane 1 (input): 293 (Human embryonic kidney epithelial cell) whole cell lysate 10ug
      Lane 2 (+): ab133533 & 293 (Human embryonic kidney epithelial cell) whole cell lysate
      Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab133533 in 293 (Human embryonic kidney epithelial cell) whole cell lysate
      For western blotting, VeriBlot for IP Detection Reagent (HRP) (ab131366) was used for detection at 1:1000 dilution.
      Blocking and diluting buffer: 5% NFDM/TBST.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab133533).

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of rat spleen tissue sections labeling TEF1/TEAD-1 with Purified  ab133533 at 1:1000 dilution (1.63 μg/ml). Heat mediated antigen retrieval was performed using Perform heat mediated antigen retrieval using EDTA Buffer, pH9.0. Tissue was counterstained with Hematoxylin. ImmunoHistoProbe one step HRP Polymer (ready to use) secondary antibody was used at 1:0 dilution. PBS instead of the primary antibody was used as the negative control.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab133533).

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse spleen tissue sections labeling TEF1/TEAD-1 with Purified  ab133533 at 1:1000 dilution (1.63 μg/ml). Heat mediated antigen retrieval was performed using Perform heat mediated antigen retrieval using EDTA Buffer, pH9.0. Tissue was counterstained with Hematoxylin. ImmunoHistoProbe one step HRP Polymer (ready to use) secondary antibody was used at 1:0 dilution. PBS instead of the primary antibody was used as the negative control.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab133533).

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human skeletal muscle tissue sections labeling TEF1/TEAD-1 with Purified ab133533 at 1:1000 dilution (1.63 μg/ml). Heat mediated antigen retrieval was performed using Perform heat mediated antigen retrieval using EDTA Buffer, pH9.0. Tissue was counterstained with Hematoxylin. ImmunoHistoProbe one step HRP Polymer (ready to use) secondary antibody was used at 1:0 dilution. PBS instead of the primary antibody was used as the negative control.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab133533).

    • Immunohistochemistry analysis of Paraffin Embedded Human placenta tissue labelling TEF1/TEAD-1 with unpurified ab133533 at 1/100.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab133533).

    • Immunohistochemistry analysis of Paraffin Embedded Human skeletal muscle tissue labelling TEF1/TEAD-1 with unpurified ab133533 at 1/100.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab133533).

    • Equilibrium disassociation constant (KD)
      Learn more about KD

      Click here to learn more about KD

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab133533).

    References

    ab219647 has not yet been referenced specifically in any publications.

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