Overview

  • Product name

    Anti-TEF1/TEAD-1 antibody [EPR3968(2)]
    See all TEF1/TEAD-1 primary antibodies
  • Description

    Rabbit monoclonal [EPR3968(2)] to TEF1/TEAD-1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, ICC/IFmore details
    Unsuitable for: Flow Cyt,IHC-P or IP
  • Species reactivity

    Reacts with: Mouse, Rat, Cow, Human
  • Immunogen

    within Human TEF1/TEAD-1 aa 1-100 (N terminal). The exact sequence is proprietary.

  • Positive control

    • HeLa, A431, 293T, and fetal muscle lysates; and HeLa cells.
  • General notes

     

     

    Previously labelled as TEF1. 

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab133535 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 52 kDa (predicted molecular weight: 48 kDa).
ICC/IF 1/50 - 1/100.
  • Application notes
    Is unsuitable for Flow Cyt,IHC-P or IP.
  • Target

    • Function

      Transcription factor which plays a key role in the Hippo signaling pathway, a pathway involved in organ size control and tumor suppression by restricting proliferation and promoting apoptosis. The core of this pathway is composed of a kinase cascade wherein MST1/MST2, in complex with its regulatory protein SAV1, phosphorylates and activates LATS1/2 in complex with its regulatory protein MOB1, which in turn phosphorylates and inactivates YAP1 oncoprotein and WWTR1/TAZ. Acts by mediating gene expression of YAP1 and WWTR1/TAZ, thereby regulating cell proliferation, migration and epithelial mesenchymal transition (EMT) induction. Binds specifically and cooperatively to the SPH and GT-IIC 'enhansons' (5'-GTGGAATGT-3') and activates transcription in vivo in a cell-specific manner. The activation function appears to be mediated by a limiting cell-specific transcriptional intermediary factor (TIF). Involved in cardiac development. Binds to the M-CAT motif.
    • Tissue specificity

      Preferentially expressed in skeletal muscle. Lower levels in pancreas, placenta, and heart.
    • Involvement in disease

      Defects in TEAD1 are the cause of Sveinsson chorioretinal atrophy (SCRA) [MIM:108985]; also known as atrophia areata (AA) or helicoidal peripapillary chorioretinal degeneration (HPCD). SCRA is characterized by symmetrical lesions radiating from the optic disk involving the retina and the choroid.
    • Sequence similarities

      Contains 1 TEA DNA-binding domain.
    • Cellular localization

      Nucleus.
    • Information by UniProt
    • Database links

    • Alternative names

      • AA antibody
      • Atrophia areata peripapillary chorioretinal degeneration antibody
      • NTEF 1 antibody
      • NTEF-1 antibody
      • NTEF1 antibody
      • Protein GT IIC antibody
      • Protein GT-IIC antibody
      • REF 1 antibody
      • REF1 antibody
      • SV40 transcriptional enhancer factor antibody
      • TCF 13 antibody
      • TCF-13 antibody
      • TCF13 antibody
      • TEA domain family member antibody
      • TEA domain family member 1 (SV40 transcriptional enhancer factor) antibody
      • TEA domain family member 1 antibody
      • TEAD 1 antibody
      • TEAD 1 protein antibody
      • TEAD-1 antibody
      • TEAD1 antibody
      • TEAD1 protein antibody
      • TEAD1_HUMAN antibody
      • TEF 1 antibody
      • TEF1 antibody
      • Transcription factor 13 (SV40 transcriptional enhancer factor) antibody
      • Transcription factor 13 antibody
      • Transcriptional enhancer factor 1 antibody
      • Transcriptional enhancer factor TEF-1 antibody
      • Transcriptional enhancer factor TEF1 antibody
      see all

    Images

    • All lanes : Anti-TEF1/TEAD-1 antibody [EPR3968(2)] (ab133535) at 1/1000 dilution

      Lane 1 : HeLa cell lysates
      Lane 2 : A431 cell lysates
      Lane 3 : 293T cell lysates
      Lane 4 : Fetal muscle lysates

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size: 48 kDa
      Observed band size: 52 kDa
      why is the actual band size different from the predicted?

    • Immunofluorescence analysis of HeLa cells labelling TEF1/TEAD-1 with ab133535 at 1/50

    • Equilibrium disassociation constant (KD)
      Learn more about KD

      Click here to learn more about KD

    References

    This product has been referenced in:

    • Wang J  et al. Effect of TEAD4 on multilineage differentiation of muscle-derived stem cells. Am J Transl Res 10:998-1011 (2018). WB ; Cow . Read more (PubMed: 29636889) »
    • Unterer B  et al. IFN-?-response mediator GBP-1 represses human cell proliferation by inhibiting the Hippo signaling transcription factor TEAD. Biochem J 475:2955-2967 (2018). Read more (PubMed: 30120107) »
    See all 2 Publications for this product

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