Product nameAnti-TEF1/TEAD-1 antibody [EPR3968(2)]
See all TEF1/TEAD-1 primary antibodies
DescriptionRabbit monoclonal [EPR3968(2)] to TEF1/TEAD-1
Tested applicationsSuitable for: WB, ICC/IFmore details
Unsuitable for: Flow Cyt,IHC-P or IP
Species reactivityReacts with: Mouse, Rat, Cow, Human
within Human TEF1/TEAD-1 aa 1-100 (N terminal). The exact sequence is proprietary.
- HeLa, A431, 293T, and fetal muscle lysates; and HeLa cells.
Previously labelled as TEF1.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Dissociation constant (KD)KD = 7.97 x 10 -10 M Learn more about KD
Storage bufferpH: 7.40
Preservative: 0.05% Sodium azide
Constituents: 40% Glycerol, 9.85% Tris glycine, 50% Tissue culture supernatant
Concentration information loading...
PurityTissue culture supernatant
Our Abpromise guarantee covers the use of ab133535 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/10000. Detects a band of approximately 52 kDa (predicted molecular weight: 48 kDa).|
|ICC/IF||1/50 - 1/100.|
FunctionTranscription factor which plays a key role in the Hippo signaling pathway, a pathway involved in organ size control and tumor suppression by restricting proliferation and promoting apoptosis. The core of this pathway is composed of a kinase cascade wherein MST1/MST2, in complex with its regulatory protein SAV1, phosphorylates and activates LATS1/2 in complex with its regulatory protein MOB1, which in turn phosphorylates and inactivates YAP1 oncoprotein and WWTR1/TAZ. Acts by mediating gene expression of YAP1 and WWTR1/TAZ, thereby regulating cell proliferation, migration and epithelial mesenchymal transition (EMT) induction. Binds specifically and cooperatively to the SPH and GT-IIC 'enhansons' (5'-GTGGAATGT-3') and activates transcription in vivo in a cell-specific manner. The activation function appears to be mediated by a limiting cell-specific transcriptional intermediary factor (TIF). Involved in cardiac development. Binds to the M-CAT motif.
Tissue specificityPreferentially expressed in skeletal muscle. Lower levels in pancreas, placenta, and heart.
Involvement in diseaseDefects in TEAD1 are the cause of Sveinsson chorioretinal atrophy (SCRA) [MIM:108985]; also known as atrophia areata (AA) or helicoidal peripapillary chorioretinal degeneration (HPCD). SCRA is characterized by symmetrical lesions radiating from the optic disk involving the retina and the choroid.
Sequence similaritiesContains 1 TEA DNA-binding domain.
- Information by UniProt
- AA antibody
- Atrophia areata peripapillary chorioretinal degeneration antibody
- NTEF 1 antibody
All lanes : Anti-TEF1/TEAD-1 antibody [EPR3968(2)] (ab133535) at 1/1000 dilution
Lane 1 : HeLa cell lysates
Lane 2 : A431 cell lysates
Lane 3 : 293T cell lysates
Lane 4 : Fetal muscle lysates
Lysates/proteins at 10 µg per lane.
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 48 kDa
Observed band size: 52 kDa why is the actual band size different from the predicted?
Immunofluorescence analysis of HeLa cells labelling TEF1/TEAD-1 with ab133535 at 1/50
Equilibrium disassociation constant (KD)
Learn more about KD
Click here to learn more about KD
This product has been referenced in:
- Wang J et al. Effect of TEAD4 on multilineage differentiation of muscle-derived stem cells. Am J Transl Res 10:998-1011 (2018). WB ; Cow . Read more (PubMed: 29636889) »
- Unterer B et al. IFN-?-response mediator GBP-1 represses human cell proliferation by inhibiting the Hippo signaling transcription factor TEAD. Biochem J 475:2955-2967 (2018). Read more (PubMed: 30120107) »