Recombinant
RabMAb

Recombinant Anti-Telethonin antibody [EPR8375] (ab133646)

Overview

  • Product name

    Anti-Telethonin antibody [EPR8375]
    See all Telethonin primary antibodies
  • Description

    Rabbit monoclonal [EPR8375] to Telethonin
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IPmore details
    Unsuitable for: ICC or IHC-P
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide corresponding to residues near the C terminus of Human Telethonin (UniProt O15273).

  • Positive control

    • Fetal muscle and fetal heart lysates
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • Dissociation constant (KD)

    KD = 1.30 x 10 -11 M
    Learn more about KD
  • Storage buffer

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
  • Purity

    Tissue culture supernatant
  • Clonality

    Monoclonal
  • Clone number

    EPR8375
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab133646 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 19 kDa.
IP 1/10 - 1/100.
  • Application notes
    Is unsuitable for ICC or IHC-P.
  • Target

    • Function

      Muscle assembly regulating factor. Mediates the antiparallel assembly of titin (TTN) molecules at the sarcomeric Z-disk.
    • Tissue specificity

      Heart and skeletal muscle.
    • Involvement in disease

      Defects in TCAP are a cause of cardiomyopathy familial hypertrophic (CMH) [MIM:192600]; also designated FHC or HCM. Familial hypertrophic cardiomyopathy is a hereditary heart disorder characterized by ventricular hypertrophy, which is usually asymmetric and often involves the interventricular septum. The symptoms include dyspnea, syncope, collapse, palpitations, and chest pain. They can be readily provoked by exercise. The disorder has inter- and intrafamilial variability ranging from benign to malignant forms with high risk of cardiac failure and sudden cardiac death.
      Defects in TCAP are a cause of limb-girdle muscular dystrophy type 2G (LGMD2G) [MIM:601954]. LGMD2G is an autosomal recessive degenerative myopathy characterized by proximal and distal muscle weakness and atrophy in the limbs, dystrophic changes on muscle biopsy, and absence of telethonin. Cardiac muscle is involved in a subset of patients.
      Defects in TCAP are the cause of cardiomyopathy dilated type 1N (CMD1N) [MIM:607487]. Dilated cardiomyopathy is a disorder characterized by ventricular dilation and impaired systolic function, resulting in congestive heart failure and arrhythmia. Patients are at risk of premature death.
    • Cellular localization

      Cytoplasm > myofibril > sarcomere.
    • Information by UniProt
    • Database links

    • Alternative names

      • 19 kDa sarcomeric protein antibody
      • CMD1N antibody
      • CMH25 antibody
      • LGMD2G antibody
      • Limb girdle muscular dystrophy 2G (autosomal recessive) antibody
      • T-cap antibody
      • Tcap antibody
      • TELE antibody
      • Telethonin antibody
      • TELT_HUMAN antibody
      • Titin cap protein antibody
      • Titin-cap antibody
      see all

    Images

    • Lane 1 : Anti-Telethonin antibody [EPR8375] (ab133646) at 1/500 dilution
      Lane 2 : Anti-Telethonin antibody [EPR8375] (ab133646) at 1/5000 dilution

      Lane 1 : Rat Muscle
      Lane 2 : Rat Heart

      Lysates/proteins at 15 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

      Predicted band size: 19 kDa
      Observed band size: 19 kDa


      Exposure time: 1 minute


      Blocking buffer 5% NFDM/TBST

      Diluting buffer 5% NFDM/TBST

    • All lanes : Anti-Telethonin antibody [EPR8375] (ab133646) at 1/5000 dilution

      Lane 1 : Human Skeletal Muscle
      Lane 2 : C2C12
      Lane 3 : Mouse Heart
      Lane 4 : Mouse Muscle

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

      Predicted band size: 19 kDa
      Observed band size: 19 kDa


      Exposure time: 1 minute


      Blocking buffer 5% NFDM/TBST

      Diluting buffer 5% NFDM/TBST

    • All lanes : Anti-Telethonin antibody [EPR8375] (ab133646) at 1/1000 dilution

      Lane 1 : Fetal muscle lysate
      Lane 2 : Fetal heart lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size: 19 kDa

    • Equilibrium disassociation constant (KD)
      Learn more about KD

      Click here to learn more about KD

    References

    ab133646 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    1-2 of 2 Abreviews or Q&A

    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (Cardiomyocyte)
    Gel Running Conditions
    Reduced Denaturing (12%)
    Loading amount
    20 µg
    Specification
    Cardiomyocyte
    Blocking step
    Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C

    Abcam user community

    Verified customer

    Submitted Oct 10 2017

    Application
    Western blot
    Loading amount
    10 µg
    Gel Running Conditions
    Reduced Denaturing (4%-12%)
    Sample
    Mouse Tissue lysate - whole (Heart)
    Specification
    Heart
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

    Abcam user community

    Verified customer

    Submitted Nov 18 2014

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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