Product nameAnti-Telomerase reverse transcriptase antibody - C-terminal
See all Telomerase reverse transcriptase primary antibodies
DescriptionRabbit polyclonal to Telomerase reverse transcriptase - C-terminal
Tested applicationsSuitable for: WB, IHC-Pmore details
Species reactivityReacts with: Human
- Human mucinous soft sarcoma tissue, Human gliomas tissue and HT29 cell lysates; Human tonsil tissue. Human ovarian and thyroid cancer tissue.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.30
Preservative: 0.05% Sodium azide
Constituents: 50% Glycerol, 49% PBS
(without Mg2+ and Ca2+)
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab183105 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/2000. Predicted molecular weight: 126 kDa.|
|IHC-P||1/50 - 1/200.|
FunctionTelomerase is a ribonucleoprotein enzyme essential for the replication of chromosome termini in most eukaryotes. Active in progenitor and cancer cells. Inactive, or very low activity, in normal somatic cells. Catalytic component of the teleromerase holoenzyme complex whose main activity is the elongation of telomeres by acting as a reverse transcriptase that adds simple sequence repeats to chromosome ends by copying a template sequence within the RNA component of the enzyme. Catalyzes the RNA-dependent extension of 3'-chromosomal termini with the 6-nucleotide telomeric repeat unit, 5'-TTAGGG-3'. The catalytic cycle involves primer binding, primer extension and release of product once the template boundary has been reached or nascent product translocation followed by further extension. More active on substrates containing 2 or 3 telomeric repeats. Telomerase activity is regulated by a number of factors including telomerase complex-associated proteins, chaperones and polypeptide modifiers. Modulates Wnt signaling. Plays important roles in aging and antiapoptosis.
Tissue specificityExpressed at a high level in thymocyte subpopulations, at an intermediate level in tonsil T lymphocytes, and at a low to undetectable level in peripheral blood T lymphocytes.
Involvement in diseaseNote=Activation of telomerase has been implicated in cell immortalization and cancer cell pathogenesis.
Defects in TERT are associated with susceptibilty to aplastic anemia (AA) [MIM:609135]. AA is a rare disease in which the reduction of the circulating blood cells results from damage to the stem cell pool in bone marrow. In most patients, the stem cell lesion is caused by an autoimmune attack. T-lymphocytes, activated by an endogenous or exogenous, and most often unknown antigenic stimulus, secrete cytokines, including IFN-gamma, which would in turn be able to suppress hematopoiesis.
Note=Genetic variations in TERT are associated with coronary artery disease (CAD).
Defects in TERT are a cause of dyskeratosis congenita autosomal dominant (ADDKC) [MIM:127550]; also known as dyskeratosis congenita Scoggins type. ADDKC is a rare, progressive bone marrow failure syndrome characterized by the triad of reticulated skin hyperpigmentation, nail dystrophy, and mucosal leukoplakia. Early mortality is often associated with bone marrow failure, infections, fatal pulmonary complications, or malignancy.
Defects in TERT are a cause of susceptibility to pulmonary fibrosis idiopathic (IPF) [MIM:178500]. Pulmonary fibrosis is a lung disease characterized by shortness of breath, radiographically evident diffuse pulmonary infiltrates, and varying degrees of inflammation and fibrosis on biopsy. It results in acute lung injury with subsequent scarring and endstage lung disease.
Sequence similaritiesBelongs to the reverse transcriptase family. Telomerase subfamily.
Contains 1 reverse transcriptase domain.
DomainThe primer grip sequence in the RT domain is required for telomerase activity and for stable association with short telomeric primers.
The RNA-interacting domain 1 (RD1)/N-terminal extension (NTE) is required for interaction with the pseudoknot-template domain of each of TERC dimers. It contains anchor sites that bind primer nucleotides upstream of the RNA-DNA hybrid and is thus an essential determinant of repeat addition processivity.
The RNA-interacting domain 2 (RD2) is essential for both interaction with the CR4-CR5 domain of TERC and for DNA sythesis.
modificationsUbiquitinated, leading to proteasomal degradation.
Phosphorylation at Tyr-707 under oxidative stress leads to translocation of TERT to the cytoplasm and reduces its antiapoptotic activity. Dephosphorylated by SHP2/PTPN11 leading to nuclear retention. Phosphorylation by the AKT pathway promotes nuclear location.
Cellular localizationNucleus > nucleolus. Nucleus > nucleoplasm. Nucleus. Chromosome > telomere. Cytoplasm. Nucleus > PML body. Shuttling between nuclear and cytoplasm depends on cell cycle, phosphorylation states, transformation and DNA damage. Diffuse localization in the nucleoplasm. Enriched in nucleoli of certain cell types. Translocated to the cytoplasm via nuclear pores in a CRM1/RAN-dependent manner involving oxidative stress-mediated phosphorylation at Tyr-707. Dephosphorylation at this site by SHP2 retains TERT in the nucleus. Translocated to the nucleus by phosphorylation by AKT.
- Information by UniProt
- CMM9 antibody
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Anti-Telomerase reverse transcriptase antibody - C-terminal (ab183105) at 1/500 dilution + Human mucinous soft sarcoma tissue lysate at 40 µg
Predicted band size: 126 kDa
Exposure time: 40 seconds
Immunohistochemical analysis of paraffin embedded Human Ovarian cancer tissue sections labeling Telomerase reverse transcriptase with ab183105 at 1/100. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. the tissue was then blocked and incubated with the antibody for one night at 4oC. A Goat Anti-Rabbit IgG H&L (HRP) at 1/200 was used as secondary.
Immunohistochemical analysis of paraffin embedded Human Thyroid cancer tissue sections labeling Telomerase reverse transcriptase with ab183105 at 1/100. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for one night at 4oC. A Goat Anti-Rabbit IgG H&L (HRP) at 1/200 was used as secondary.
Immunohistochemical analysis of paraffin embedded Human tonsil tissue labeling Telomerase reverse transcriptase with ab183105 at 1/30. Image on the right is treated with the synthetic peptide.
ab183105 has been referenced in 9 publications.
- Potharaju M et al. Clinicopathological Analysis of HIF-1alpha and TERT on Survival Outcome in Glioblastoma Patients: A Prospective, Single Institution Study. J Cancer 10:2397-2406 (2019). PubMed: 31258744
- Savelyev N et al. Comprehensive analysis of telomerase inhibition by gallotannin. Oncotarget 9:18712-18719 (2018). PubMed: 29721155
- Yang H et al. Concomitant underexpression of TGFBR2 and overexpression of hTERT are associated with poor prognosis in cervical cancer. Sci Rep 7:41670 (2017). WB, IHC-P ; Human . PubMed: 28195144
- Zou MX et al. Upregulated human telomerase reverse transcriptase (hTERT) expression is associated with spinal chordoma growth, invasion and poor prognosis. Am J Transl Res 8:516-29 (2016). PubMed: 27158344
- Suryo Rahmanto Y et al. Inactivating ARID1A Tumor Suppressor Enhances TERT Transcription and Maintains Telomere Length in Cancer Cells. J Biol Chem 291:9690-9 (2016). PubMed: 26953344
- Song Y et al. Novel 1, 3-N, O-Spiroheterocyclic compounds inhibit heparanase activity and enhance nedaplatin-induced cytotoxicity in cervical cancer cells. Oncotarget 7:36154-36167 (2016). PubMed: 27166252
- Patel PL et al. Derepression of hTERT gene expression promotes escape from oncogene-induced cellular senescence. Proc Natl Acad Sci U S A 113:E5024-33 (2016). ICC/IF ; Human . PubMed: 27503890
- Liu Q et al. TERT alleviates irradiation-induced late rectal injury by reducing hypoxia-induced ROS levels through the activation of NF-?B and autophagy. Int J Mol Med 38:785-93 (2016). IHC-P ; Mouse . PubMed: 27431814
- Naidoo K et al. Knock-Down of the 37kDa/67kDa Laminin Receptor LRP/LR Impedes Telomerase Activity. PLoS One 10:e0141618 (2015). PubMed: 26545108