Overview

  • Product name
    Anti-Telomerase reverse transcriptase antibody [Y182]
    See all Telomerase reverse transcriptase primary antibodies
  • Description
    Rabbit monoclonal [Y182] to Telomerase reverse transcriptase
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, Flow Cyt, IP, IHC-P, IHC-Frmore details
    Unsuitable for: ICC/IF
  • Species reactivity
    Reacts with: Cow, Human
  • Immunogen

    Synthetic peptide within Human Telomerase reverse transcriptase aa 1100-1200 (C terminal). The exact sequence is proprietary.

  • Positive control
    • WB: Hela cell lysate. IHC-P: Human thymus tissue.
  • General notes

    Mouse: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab32020 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Detects a band of approximately 122 kDa (predicted molecular weight: 127 kDa).
Flow Cyt 1/100.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 

IP Use at an assay dependent concentration.
IHC-P Use at an assay dependent concentration. PubMed: 20367640
IHC-Fr Use a concentration of 0.75 µg/ml. PubMed: 17982423
  • Application notes
    Is unsuitable for ICC/IF.
  • Target

    • Function
      Telomerase is a ribonucleoprotein enzyme essential for the replication of chromosome termini in most eukaryotes. Active in progenitor and cancer cells. Inactive, or very low activity, in normal somatic cells. Catalytic component of the teleromerase holoenzyme complex whose main activity is the elongation of telomeres by acting as a reverse transcriptase that adds simple sequence repeats to chromosome ends by copying a template sequence within the RNA component of the enzyme. Catalyzes the RNA-dependent extension of 3'-chromosomal termini with the 6-nucleotide telomeric repeat unit, 5'-TTAGGG-3'. The catalytic cycle involves primer binding, primer extension and release of product once the template boundary has been reached or nascent product translocation followed by further extension. More active on substrates containing 2 or 3 telomeric repeats. Telomerase activity is regulated by a number of factors including telomerase complex-associated proteins, chaperones and polypeptide modifiers. Modulates Wnt signaling. Plays important roles in aging and antiapoptosis.
    • Tissue specificity
      Expressed at a high level in thymocyte subpopulations, at an intermediate level in tonsil T lymphocytes, and at a low to undetectable level in peripheral blood T lymphocytes.
    • Involvement in disease
      Note=Activation of telomerase has been implicated in cell immortalization and cancer cell pathogenesis.
      Defects in TERT are associated with susceptibilty to aplastic anemia (AA) [MIM:609135]. AA is a rare disease in which the reduction of the circulating blood cells results from damage to the stem cell pool in bone marrow. In most patients, the stem cell lesion is caused by an autoimmune attack. T-lymphocytes, activated by an endogenous or exogenous, and most often unknown antigenic stimulus, secrete cytokines, including IFN-gamma, which would in turn be able to suppress hematopoiesis.
      Note=Genetic variations in TERT are associated with coronary artery disease (CAD).
      Defects in TERT are a cause of dyskeratosis congenita autosomal dominant (ADDKC) [MIM:127550]; also known as dyskeratosis congenita Scoggins type. ADDKC is a rare, progressive bone marrow failure syndrome characterized by the triad of reticulated skin hyperpigmentation, nail dystrophy, and mucosal leukoplakia. Early mortality is often associated with bone marrow failure, infections, fatal pulmonary complications, or malignancy.
      Defects in TERT are a cause of susceptibility to pulmonary fibrosis idiopathic (IPF) [MIM:178500]. Pulmonary fibrosis is a lung disease characterized by shortness of breath, radiographically evident diffuse pulmonary infiltrates, and varying degrees of inflammation and fibrosis on biopsy. It results in acute lung injury with subsequent scarring and endstage lung disease.
    • Sequence similarities
      Belongs to the reverse transcriptase family. Telomerase subfamily.
      Contains 1 reverse transcriptase domain.
    • Domain
      The primer grip sequence in the RT domain is required for telomerase activity and for stable association with short telomeric primers.
      The RNA-interacting domain 1 (RD1)/N-terminal extension (NTE) is required for interaction with the pseudoknot-template domain of each of TERC dimers. It contains anchor sites that bind primer nucleotides upstream of the RNA-DNA hybrid and is thus an essential determinant of repeat addition processivity.
      The RNA-interacting domain 2 (RD2) is essential for both interaction with the CR4-CR5 domain of TERC and for DNA sythesis.
    • Post-translational
      modifications
      Ubiquitinated, leading to proteasomal degradation.
      Phosphorylation at Tyr-707 under oxidative stress leads to translocation of TERT to the cytoplasm and reduces its antiapoptotic activity. Dephosphorylated by SHP2/PTPN11 leading to nuclear retention. Phosphorylation by the AKT pathway promotes nuclear location.
    • Cellular localization
      Nucleus > nucleolus. Nucleus > nucleoplasm. Nucleus. Chromosome > telomere. Cytoplasm. Nucleus > PML body. Shuttling between nuclear and cytoplasm depends on cell cycle, phosphorylation states, transformation and DNA damage. Diffuse localization in the nucleoplasm. Enriched in nucleoli of certain cell types. Translocated to the cytoplasm via nuclear pores in a CRM1/RAN-dependent manner involving oxidative stress-mediated phosphorylation at Tyr-707. Dephosphorylation at this site by SHP2 retains TERT in the nucleus. Translocated to the nucleus by phosphorylation by AKT.
    • Information by UniProt
    • Database links
    • Alternative names
      • CMM9 antibody
      • DKCA2 antibody
      • DKCB4 antibody
      • EST2 antibody
      • HEST2 antibody
      • htert antibody
      • hTRT antibody
      • PFBMFT1 antibody
      • TCS1 antibody
      • Telomerase associated protein 2 antibody
      • Telomerase catalytic subunit antibody
      • Telomerase reverse transcriptase antibody
      • Telomerase-associated protein 2 antibody
      • Telomere Reverse Transcriptase antibody
      • TERT antibody
      • TERT_HUMAN antibody
      • TP2 antibody
      • TRT antibody
      see all

    Images

    • Telomerase reverse transcriptase (TERT) expression increased in PH patients

      TERT protein staining. TERT protein level was increased in the pulmonary vascular wall in Pulmonary hypertension (PH) patients (n = 5). In the animal experiments (n = 6), hypoxia or MCT administration significantly increased the expression of TERT protein compared with control rats, which were reversible by administration of azidothymidine (AZT, 20 mg/kg body weight).

      Telomerase reverse transcriptase was detected using ab32020 at 1/50 dilution in 4% paraformaldehyde-fixed, paraffin-embedded lung tissue.

      (From Figure 2A of Shen et al)

    • Anti-Telomerase reverse transcriptase antibody [Y182] (ab32020) at 1/1000 dilution + Hela (human epithelial cell line from cervix adenocarcinoma) cell lysate

      Predicted band size: 127 kDa
      Observed band size: 122 kDa
      why is the actual band size different from the predicted?

    • ab32020 staining Telomerase reverse transcriptase in human thymus tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% hydrogen peroxide for 10 minutes at 25°C; antigen retrieval was by heat mediation in citrate buffer pH6.0 at 100°C for 20 minutes. Samples were incubated with primary antibody (1/100) for 20 minutes at 25°C. An undiluted HRP polymer-conjugated goat anti-rabbit IgG polyclonal was used as the secondary antibody.

      See Abreview

    References

    This product has been referenced in:
    • Guzman H  et al. miR-128 inhibits telomerase activity by targeting TERT mRNA. Oncotarget 9:13244-13253 (2018). Read more (PubMed: 29568354) »
    • Li G  et al. Alternative splicing of human telomerase reverse transcriptase in gliomas and its modulation mediated by CX-5461. J Exp Clin Cancer Res 37:78 (2018). Read more (PubMed: 29631594) »
    See all 50 Publications for this product

    Customer reviews and Q&As

    1-10 of 25 Abreviews or Q&A

    Answer

    Thank you for contacting us.

    I would like to suggestthe followingtwo antibodies which are suitable for your customer's purpose:

    https://www.abcam.com/index.html?datasheet=5181 (or use the following: https://www.abcam.com/index.html?datasheet=5181).

    ab5181 Anti-Telomerase reverse transcriptase antibody [2C4]has one reference with the use in ICC.

    https://www.abcam.com/index.html?datasheet=32020 (or use the following: https://www.abcam.com/index.html?datasheet=32020).

    ab32020 Anti-Telomerase reverse transcriptase antibody [Y182]has two customer raitings and one reference for the use in ICC.


    I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

    Use our products? Submit an Abreview. Earn rewards!
    https://www.abcam.com/abreviews

    Read More
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Blocking step
    hydrogen peroxide as blocking agent for 10 minute(s) · Concentration: 3% · Temperature: 25°C
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Citrate, pH6.0, 100C, 20 min
    Sample
    Human Tissue sections (thymus)
    Specification
    thymus
    Permeabilization
    No
    Fixative
    Formaldehyde

    Abcam user community

    Verified customer

    Submitted Jul 30 2014

    Application
    Western blot
    Loading amount
    25000 cells
    Gel Running Conditions
    Non-reduced Non-Denaturing (Native) (8)
    Sample
    Human Cell lysate - whole cell (prostate)
    Specification
    prostate
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

    Abcam user community

    Verified customer

    Submitted Jul 30 2014

    Abcam has not validated the combination of species/application used in this Abreview.
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Blocking step
    Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Dako antigen retrieval solution
    Sample
    Marmoset (common) Tissue sections (Adult testis)
    Specification
    Adult testis
    Permeabilization
    No
    Fixative
    Formaldehyde

    Dr. 煜欽 林

    Verified customer

    Submitted Jun 21 2013

    Question
    Answer

    Thank you for contacting us.

    Unfortunately we do not have any telomerase antibodies tested and known to cross-react with dog. However, we have some telomerase and telomerase reverse transcriptase antibodies that showed some sequence homology with dog.

    ab32020, Anti-Telomerase reverse transcriptase antibody [Y182], 75% homology with dog
    ab94523, Anti-Telomerase reverse transcriptase antibody, 76% homology with dog
    ab63370, Anti-Telomerase antibody, 80% homology with dog
    ab130125, Anti-Telomerase reverse transcriptase antibody, 86% homology with dog

    Generally we say anything with > 85% sequence homology should cross-react, so we have a few that are right on the edge.

    If you would like to test one of these antibodies in dog, you could qualify for our AbTrial where you purchase the antibody at cost, test it in dog, submit your results (positive or negative) in the form of an Abreview, and you receive the price you paid for the antibody off a future purchase. More info here:

    https://www.abcam.com/index.html?pageconfig=resource&rid=11998

    I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information of if you'd like to participate in the AbTrial.

    Read More

    Answer

    Thank you for contacting us. Yes, this is the same product. Epitomics is now part of Abcam so you will see the same products in our catalog from theirs. Please let me know if you have any further questions.

    Read More
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Human Cell (corneal endothelium)
    Permeabilization
    No
    Specification
    corneal endothelium
    Blocking step
    Serum as blocking agent for 30 minute(s) · Concentration: 100% · Temperature: 37°C
    Fixative
    Formaldehyde

    Dr. Zhiguo He

    Verified customer

    Submitted Nov 01 2012

    Answer

    Gracias por tu respuesta.

    Siento mucho que no consiguierais solucionar los problemas de tinción del anticuerpo. No hay ningún problema en mandaros de forma gratuita el anticuerpo ab5181. Este anticuerpo se ha validado en IHC-P (con tejidos humanos) dando buenos resultados, y por tanto también está garantizado (durante 6 meses) en esta aplicación.

    Si me confirmas que deseas recibirlo realizo de inmediato el pedido para que os llegue cuanto antes.

    Espero entonces tu respuesta para poder solucionar este inconveniente. Gracias por tu colaboración.

    Read More

    Question
    Answer

    Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products.
    I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has caused. As requested, I have issued a free of charge replacement.
    To check the status of the order please contact our Customer Service team and reference this number.
    Please note that this free of charge replacement vial is also covered by our Abpromise guarantee. Should you still be experiencing difficulties, or if you have any further questions, please do not hesitate to let us know.
    I wish you the best of luck with your research.

    Read More

    Answer

    Thank you very much for your interest in our antibodies.

    As an Oct4 antibody, I can recommend the ab19857 for use in ChIP. It has been tested to workgoodin ChIP andto recognize thehuman, mouse and rhesus monkey protein. https://www.abcam.com/index.html?datasheet=19857 (or use the following: https://www.abcam.com/index.html?datasheet=19857).

    To detect DNMT3B in ChIP, we dohave three antibodies tested and guaranteed thus in ChIP (ab13604, ab2851, ab122932). I canrecommend theab13604, which is testedagainst the human and mouse protein and is also cited in several publication. https://www.abcam.com/index.html?datasheet=13604 (or use the following: https://www.abcam.com/index.html?datasheet=13604).

    In regards to the anti Telomerase reverse transcriptase antibody, we do currently not have an antibody which is tested in ChIP. However we can provide you with a testing discount if you would like to test one of our anti Telomerase reverse transcriptase antibodies in ChIP. Please see here below for the details of this offer. For example the antibody ab32020 has already been tested in immunoprecipitation and many other applications, such as WB, ICC/IF, Flow Cyt, IHC-P, ICC, IHC-Fr. It is therefore likely to work in ChIP, although we can not guarantee it. The ab32020 has been tested already against Cow andHuman. https://www.abcam.com/index.html?datasheet=32020 (or use the following: https://www.abcam.com/index.html?datasheet=32020).

    I hope this information is helpful. Please do not hesitate to contact me again, should you have any other questions.



    Testing offer:

    If you would like to test the ab32020 in ChIP, I can offer a discount off a future purchase if you buyab32020 now, test it inChIP and submit feedback to us in the form of an Abreview. It doesn’t matter whether the Abreview is positive or negative, we would just really like to receive your feedback. The discount would be to the value of: 1 free primary antibody.

    If you are interested in this offer, please follow these steps:

    1. Reply to this e-mail to let me know that you would like to proceed and testab32020 in ChIP. I will then send a discount code. This code must be issued before purchasing ab32020 so please wait for my reply before ordering.

    2. Purchase ab32020 either by phone, fax, or online (www.abcam.com).

    3. Test it in ChIP.

    4. Let us know the results, positive or negative, using our Abreview system (this will take about 10 minutes and images are great if you have them!). To find out how to submit an Abreview, please visit: https://www.abcam.com/abreviews.

    5. After the review is submitted to us, the discount code becomes active. Simply place your new order by phone, fax, or on the web and mention the discount code. The discount can be redeemed for anyprimary antibodyordered and the discount code is valid for 4 months after issue.
    Please remember that submission of the Abreview is sufficient for the discount code to become active.

    We are always pleased to obtain feedback about our products and any information is greatly appreciated! Even if ab32020 turns out to be unsuitable for ChIP, you will still receive the discount on your next purchase after your Abreview has been submitted.

    Please let me know if you have any questions about this offer and I would be happy to help you further.

    The Terms and Conditions of this offer can be found at: www.abcam.com/collaborationdiscount.

    Read More

    1-10 of 25 Abreviews or Q&A

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