Anti-Telomerase reverse transcriptase antibody [Y182] (ab32020)


  • Product name
    Anti-Telomerase reverse transcriptase antibody [Y182]
    See all Telomerase reverse transcriptase primary antibodies
  • Description
    Rabbit monoclonal [Y182] to Telomerase reverse transcriptase
  • Host species
  • Tested applications
    Suitable for: WB, Flow Cyt, IP, IHC-P, IHC-Frmore details
    Unsuitable for: ICC/IF
  • Species reactivity
    Reacts with: Cow, Human
  • Immunogen

    Synthetic peptide within Human Telomerase reverse transcriptase aa 1100-1200 (C terminal). The exact sequence is proprietary.

  • Positive control
    • WB: Hela cell lysate. IHC-P: Human lung adenocarcinoma.
  • General notes

    Mouse: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.


    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.



Our Abpromise guarantee covers the use of ab32020 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Detects a band of approximately 122 kDa (predicted molecular weight: 127 kDa).
Flow Cyt 1/100.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.


IP Use at an assay dependent concentration.
IHC-P Use at an assay dependent concentration. PubMed: 20367640
IHC-Fr Use a concentration of 0.75 µg/ml. PubMed: 17982423
  • Application notes
    Is unsuitable for ICC/IF.
  • Target

    • Function
      Telomerase is a ribonucleoprotein enzyme essential for the replication of chromosome termini in most eukaryotes. Active in progenitor and cancer cells. Inactive, or very low activity, in normal somatic cells. Catalytic component of the teleromerase holoenzyme complex whose main activity is the elongation of telomeres by acting as a reverse transcriptase that adds simple sequence repeats to chromosome ends by copying a template sequence within the RNA component of the enzyme. Catalyzes the RNA-dependent extension of 3'-chromosomal termini with the 6-nucleotide telomeric repeat unit, 5'-TTAGGG-3'. The catalytic cycle involves primer binding, primer extension and release of product once the template boundary has been reached or nascent product translocation followed by further extension. More active on substrates containing 2 or 3 telomeric repeats. Telomerase activity is regulated by a number of factors including telomerase complex-associated proteins, chaperones and polypeptide modifiers. Modulates Wnt signaling. Plays important roles in aging and antiapoptosis.
    • Tissue specificity
      Expressed at a high level in thymocyte subpopulations, at an intermediate level in tonsil T lymphocytes, and at a low to undetectable level in peripheral blood T lymphocytes.
    • Involvement in disease
      Note=Activation of telomerase has been implicated in cell immortalization and cancer cell pathogenesis.
      Defects in TERT are associated with susceptibilty to aplastic anemia (AA) [MIM:609135]. AA is a rare disease in which the reduction of the circulating blood cells results from damage to the stem cell pool in bone marrow. In most patients, the stem cell lesion is caused by an autoimmune attack. T-lymphocytes, activated by an endogenous or exogenous, and most often unknown antigenic stimulus, secrete cytokines, including IFN-gamma, which would in turn be able to suppress hematopoiesis.
      Note=Genetic variations in TERT are associated with coronary artery disease (CAD).
      Defects in TERT are a cause of dyskeratosis congenita autosomal dominant (ADDKC) [MIM:127550]; also known as dyskeratosis congenita Scoggins type. ADDKC is a rare, progressive bone marrow failure syndrome characterized by the triad of reticulated skin hyperpigmentation, nail dystrophy, and mucosal leukoplakia. Early mortality is often associated with bone marrow failure, infections, fatal pulmonary complications, or malignancy.
      Defects in TERT are a cause of susceptibility to pulmonary fibrosis idiopathic (IPF) [MIM:178500]. Pulmonary fibrosis is a lung disease characterized by shortness of breath, radiographically evident diffuse pulmonary infiltrates, and varying degrees of inflammation and fibrosis on biopsy. It results in acute lung injury with subsequent scarring and endstage lung disease.
    • Sequence similarities
      Belongs to the reverse transcriptase family. Telomerase subfamily.
      Contains 1 reverse transcriptase domain.
    • Domain
      The primer grip sequence in the RT domain is required for telomerase activity and for stable association with short telomeric primers.
      The RNA-interacting domain 1 (RD1)/N-terminal extension (NTE) is required for interaction with the pseudoknot-template domain of each of TERC dimers. It contains anchor sites that bind primer nucleotides upstream of the RNA-DNA hybrid and is thus an essential determinant of repeat addition processivity.
      The RNA-interacting domain 2 (RD2) is essential for both interaction with the CR4-CR5 domain of TERC and for DNA sythesis.
    • Post-translational
      Ubiquitinated, leading to proteasomal degradation.
      Phosphorylation at Tyr-707 under oxidative stress leads to translocation of TERT to the cytoplasm and reduces its antiapoptotic activity. Dephosphorylated by SHP2/PTPN11 leading to nuclear retention. Phosphorylation by the AKT pathway promotes nuclear location.
    • Cellular localization
      Nucleus > nucleolus. Nucleus > nucleoplasm. Nucleus. Chromosome > telomere. Cytoplasm. Nucleus > PML body. Shuttling between nuclear and cytoplasm depends on cell cycle, phosphorylation states, transformation and DNA damage. Diffuse localization in the nucleoplasm. Enriched in nucleoli of certain cell types. Translocated to the cytoplasm via nuclear pores in a CRM1/RAN-dependent manner involving oxidative stress-mediated phosphorylation at Tyr-707. Dephosphorylation at this site by SHP2 retains TERT in the nucleus. Translocated to the nucleus by phosphorylation by AKT.
    • Information by UniProt
    • Database links
    • Alternative names
      • CMM9 antibody
      • DKCA2 antibody
      • DKCB4 antibody
      • EST2 antibody
      • HEST2 antibody
      • htert antibody
      • hTRT antibody
      • PFBMFT1 antibody
      • TCS1 antibody
      • Telomerase associated protein 2 antibody
      • Telomerase catalytic subunit antibody
      • Telomerase reverse transcriptase antibody
      • Telomerase-associated protein 2 antibody
      • Telomere Reverse Transcriptase antibody
      • TERT antibody
      • TERT_HUMAN antibody
      • TP2 antibody
      • TRT antibody
      see all


    • ab32020 staining Telomerase reverse transcriptase in human thymus tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% hydrogen peroxide for 10 minutes at 25°C; antigen retrieval was by heat mediation in citrate buffer pH6.0 at 100°C for 20 minutes. Samples were incubated with primary antibody (1/100) for 20 minutes at 25°C. An undiluted HRP polymer-conjugated goat anti-rabbit IgG polyclonal was used as the secondary antibody.

      See Abreview

    • Anti-Telomerase reverse transcriptase antibody [Y182] (ab32020) at 1/1000 dilution + Hela cell lysate

      Predicted band size: 127 kDa
      Observed band size: 122 kDa (why is the actual band size different from the predicted?)


    This product has been referenced in:
    • Guzman H  et al. miR-128 inhibits telomerase activity by targeting TERT mRNA. Oncotarget 9:13244-13253 (2018). Read more (PubMed: 29568354) »
    • Li G  et al. Alternative splicing of human telomerase reverse transcriptase in gliomas and its modulation mediated by CX-5461. J Exp Clin Cancer Res 37:78 (2018). Read more (PubMed: 29631594) »

    See all 46 Publications for this product

    Customer reviews and Q&As

    Thank you for contacting us.

    I would like to suggestthe followingtwo antibodies which are suitable for your customer's purpose: (or use the following:

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    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Blocking step
    hydrogen peroxide as blocking agent for 10 minute(s) · Concentration: 3% · Temperature: 25°C
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Citrate, pH6.0, 100C, 20 min
    Human Tissue sections (thymus)

    Abcam user community

    Verified customer

    Submitted Jul 30 2014

    Western blot
    Loading amount
    25000 cells
    Gel Running Conditions
    Non-reduced Non-Denaturing (Native) (8)
    Human Cell lysate - whole cell (prostate)
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

    Abcam user community

    Verified customer

    Submitted Jul 30 2014

    Abcam has not validated the combination of species/application used in this Abreview.
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Blocking step
    Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Dako antigen retrieval solution
    Marmoset (common) Tissue sections (Adult testis)
    Adult testis

    Dr. 煜欽 林

    Verified customer

    Submitted Jun 21 2013

    Thank you for contacting us.

    Unfortunately we do not have any telomerase antibodies tested and known to cross-react with dog. However, we have some telomerase and telomerase reverse transcriptase antibodies that showed some sequence homology...

    Read More

    Thank you for contacting us. Yes, this is the same product. Epitomics is now part of Abcam so you will see the same products in our catalog from theirs. Please let me know if you have any further questions.

    Immunocytochemistry/ Immunofluorescence
    Human Cell (corneal endothelium)
    corneal endothelium
    Blocking step
    Serum as blocking agent for 30 minute(s) · Concentration: 100% · Temperature: 37°C

    Dr. Zhiguo He

    Verified customer

    Submitted Nov 01 2012

    Gracias por tu respuesta.

    Siento mucho que no consiguierais solucionar los problemas de tinción del anticuerpo. No hay ningún problema en mandaros de forma gratuita el anticuerpo ab5181. Este anticuerpo se ha validado en IHC-P (...

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    Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products.
    I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has c...

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    Thank you very much for your interest in our antibodies.

    As an Oct4 antibody, I can recommend the ab19857 for use in ChIP. It has been tested to workgoodin ChIP andto recognize thehuman, mouse and rhesus monkey protein.

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    1-10 of 25 Abreviews or Q&A


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