Immunocytochemistry/ Immunofluorescence abreview for Anti-Telomerase reverse transcriptase antibody [Y182]

Good
Abreviews
This product is known to not work in this application or species.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (corneal endothelium)
Permeabilization
No
Specification
corneal endothelium
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 100% · Temperature: 37°C
Fixative
Formaldehyde

Other product details

Incubation time
1 hour(s) and 0 minute(s) · Temperature: 37°C · Diluent: 2%BSA, 2%goat serum
Dilution
1/200

Secondary antibody

Dilution
1/500
Name
Non-Abcam antibody was used: Alexa fluor 555 goat anti rabbit
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor­ 555

Additional data

Additional Notes
TRT is expressed in cytoplasm of corneal endothelial cells in extreme periphery. 0.5% SDS is nècessary to be performed after 0.5% PFA fixation.
PubMed ID
22949402: View

Dr. Zhiguo He

Verified customer

Submitted Nov 01 2012

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