Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Telomerase reverse transcriptase antibody [Y182]

Average
Abreviews
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step
hydrogen peroxide as blocking agent for 10 minute(s) · Concentration: 3% · Temperature: 25°C
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citrate, pH6.0, 100C, 20 min
Sample
Human Tissue sections (thymus)
Specification
thymus
Permeabilization
No
Fixative
Formaldehyde

Other product details

Incubation time
20 minute(s) · Temperature: 25°C · Diluent: Dako diluent, background reducers
Dilution
1/100

Secondary antibody

Name
Non-Abcam antibody was used: Leica Refine anti-mouse/rabbit
Host species: Goat
Clonality: Polyclonal
Conjugation: HRP polymer

Additional data

Additional Notes
autmoted on Leica Bond various treatment regims tested citrate gave best signal/background Hassal's Corpuscles stain strongly with TERT interestingly no thymocytes stain.

Abcam user community

Verified customer

Submitted Jul 30 2014

Sign up