Anti-TEM1 antibody (ab217535)
Key features and details
- Rabbit polyclonal to TEM1
- Suitable for: IHC-P, Flow Cyt
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-TEM1 antibody
See all TEM1 primary antibodies -
Description
Rabbit polyclonal to TEM1 -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, Flow Cytmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat -
Immunogen
Synthetic peptide within Human TEM1 aa 250-350 conjugated to keyhole limpet haemocyanin. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
Database link: Q9HCU0 -
Positive control
- Human colon carcinoma tissue; 293T cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Proclin 300
Constituents: 50% Glycerol (glycerin, glycerine), 1% BSA, 48.98% TBS, 1X -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab217535 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-P |
1/100 - 1/500.
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Flow Cyt |
1/20 - 1/100.
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Notes |
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IHC-P
1/100 - 1/500. |
Flow Cyt
1/20 - 1/100. |
Target
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Function
May play a role in tumor angiogenesis. -
Tissue specificity
Expressed in tumor endothelial cells but absent or barely detectable in normal endothelial cells. Expressed in metastatic lesions of the liver and during angiogenesis of corpus luteum formation and wound healing. Expressed in vascular endothelial cells of malignant tumors but not in normal blood vessels. Expressed in stromal fibroblasts. -
Sequence similarities
Contains 1 C-type lectin domain.
Contains 1 EGF-like domain.
Contains 1 Sushi (CCP/SCR) domain. -
Post-translational
modificationsO-glycosylated with sialylated oligosaccharides.
May be N-glycosylated. -
Cellular localization
Membrane. - Information by UniProt
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Database links
- Entrez Gene: 57124 Human
- Entrez Gene: 70445 Mouse
- Entrez Gene: 293669 Rat
- Omim: 606064 Human
- SwissProt: Q9HCU0 Human
- SwissProt: Q91V98 Mouse
- Unigene: 195727 Human
- Unigene: 29597 Mouse
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Alternative names
- CD164 sialomucin like 1 antibody
- CD164L1 antibody
- CD248 antibody
see all
Images
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Immunohistochemical analysis of formalin-fixed, paraffin-embedded human colon carcinoma tissue labeling TEM1 with ab217535 at 1/200 dilution, followed by conjugation to the secondary antibody and DAB staining.
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Immunohistochemical analysis of formalin-fixed, paraffin-embedded human colon carcinoma tissue labeling TEM1 with ab217535 at 1/200 dilution, followed by conjugation to the secondary antibody and DAB staining.
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Flow cytometric analysis of 293T cells labeling TEM1 with ab217535 at 1/50 dilution for 40 minutes followed by a conjugated secondary (green), compared to control cells (blue).
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab217535 has not yet been referenced specifically in any publications.