Product nameAnti-TEM1 antibody
See all TEM1 primary antibodies
DescriptionRabbit polyclonal to TEM1
Specificityab67273 recognizes both isoforms of TEM1.
Tested applicationsSuitable for: ICC/IF, WB, IHC-Pmore details
Species reactivityReacts with: Mouse, Rat, Human
A 14 amino acid synthetic peptide derived from near the carboxy terminus of the human TEM1.
- Human colon tissue lysate and human colon tissue.
Storage instructionsShipped at 4°C. Store at +4°C.
Storage bufferPreservative: 0.02% Sodium azide
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab67273 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: Use at a concentration of 0.5 - 1 µg/ml. Detects a band of approximately 85 & 50 kDa (predicted molecular weight: 80 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
FunctionMay play a role in tumor angiogenesis.
Tissue specificityExpressed in tumor endothelial cells but absent or barely detectable in normal endothelial cells. Expressed in metastatic lesions of the liver and during angiogenesis of corpus luteum formation and wound healing. Expressed in vascular endothelial cells of malignant tumors but not in normal blood vessels. Expressed in stromal fibroblasts.
Sequence similaritiesContains 1 C-type lectin domain.
Contains 1 EGF-like domain.
Contains 1 Sushi (CCP/SCR) domain.
modificationsO-glycosylated with sialylated oligosaccharides.
May be N-glycosylated.
- Information by UniProt
- CD164 sialomucin like 1 antibody
- CD164L1 antibody
- CD248 antibody
Immunofluorescence of TEM1 in Human Colon cells using ab67273 at 20 ug/ml.
Lane 1 : Anti-TEM1 antibody (ab67273) at 0.5 µg/ml
Lane 2 : Anti-TEM1 antibody (ab67273) at 1 µg/ml
All lanes : Human colon tissue lysate
Lysates/proteins at 15 µg per lane.
All lanes : anti rabbit secondary antibody
Predicted band size: 50, 80 kDa
Observed band size: 50,80 kDa why is the actual band size different from the predicted?
Additional bands at: 100 kDa. We are unsure as to the identity of these extra bands.
ab67273 at 2.5 μg/ml.in staining of TEM1 in human colon tissue using IHC-P.
ab67273 has not yet been referenced specifically in any publications.