Overview

  • Product name

  • Description

    Rabbit polyclonal to TEM1
  • Host species

    Rabbit
  • Specificity

    ab67273 recognizes both isoforms of TEM1.
  • Tested applications

    Suitable for: ICC/IF, WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    A 14 amino acid synthetic peptide derived from near the carboxy terminus of the human TEM1.

  • Positive control

    • Human colon tissue lysate and human colon tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab67273 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF
WB
IHC-P
  • Application notes
    IHC-P: Use at an assay dependent dilution.
    WB: Use at a concentration of 0.5 - 1 µg/ml. Detects a band of approximately 85 & 50 kDa (predicted molecular weight: 80 kDa).


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Function

      May play a role in tumor angiogenesis.
    • Tissue specificity

      Expressed in tumor endothelial cells but absent or barely detectable in normal endothelial cells. Expressed in metastatic lesions of the liver and during angiogenesis of corpus luteum formation and wound healing. Expressed in vascular endothelial cells of malignant tumors but not in normal blood vessels. Expressed in stromal fibroblasts.
    • Sequence similarities

      Contains 1 C-type lectin domain.
      Contains 1 EGF-like domain.
      Contains 1 Sushi (CCP/SCR) domain.
    • Post-translational
      modifications

      O-glycosylated with sialylated oligosaccharides.
      May be N-glycosylated.
    • Cellular localization

      Membrane.
    • Information by UniProt
    • Database links

    • Alternative names

      • CD164 sialomucin like 1 antibody
      • CD164L1 antibody
      • CD248 antibody
      • CD248 antigen endosialin antibody
      • CD248 molecule endosialin antibody
      • CD248_HUMAN antibody
      • Endosialin antibody
      • TEM 1 antibody
      • TEM1 antibody
      • Tumor endothelial marker 1 antibody
      see all

    Images

    • Immunofluorescence of TEM1 in Human Colon cells using ab67273 at 20 ug/ml.

    • Lane 1 : Anti-TEM1 antibody (ab67273) at 0.5 µg/ml
      Lane 2 : Anti-TEM1 antibody (ab67273) at 1 µg/ml

      All lanes : Human colon tissue lysate

      Lysates/proteins at 15 µg per lane.

      Secondary
      All lanes : anti rabbit secondary antibody

      Predicted band size: 50, 80 kDa
      Observed band size: 50,80 kDa
      why is the actual band size different from the predicted?
      Additional bands at: 100 kDa. We are unsure as to the identity of these extra bands.

    • ab67273 at 2.5 μg/ml.in staining of TEM1 in human colon tissue using IHC-P.

    References

    ab67273 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    1-3 of 3 Abreviews or Q&A

    Question
    Answer

    Thank you for contacting us yesterday and alerting us to the problem you are experiencing with the anti-TEM1 antibody (ab67273). We take product complaints very seriously, and investigate every product that we feel may not be performing correctly.

    As discussed over the phone, all of our products are covered by the Abpromise guarantee. This stipulates that if you find the product is not functioning as indicated on the datasheet of the product and the product was purchased in the last six months and is being used according to our Abpromise, we would be happy to replace or refund the antibody. As you mentioned that you were not able to test the antibody at the time of purchase I would be willing to extend this guarantee period for you as a gesture of good will. More information on the Abpromise guarantee can be found from the following link:

    www.abcam.com/abpromise

    When you are ready to share the data you have gathered with this antibody please do let me know and I can look into this further. I have attached a questionnaire to this email to provide a guideline as to the information which is usually very useful in investigating this case internally and initiate additional testing where necessary.

    You also mentioned over the phone that your colleague had had similar problems with the
    anti-TEM1 antibody (ab67275). If you could provide any information as to the results generated with this antibody I would be very interested to know.

    I look forward to receiving your reply.

    Read More
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Mouse Tissue sections (skin)
    Specification
    skin
    Fixative
    Paraformaldehyde
    Antigen retrieval step
    Enzymatic - Buffer/Enzyme Used: proteinase K
    Permeabilization
    No
    Blocking step
    Serum as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: RT°C

    Abcam user community

    Verified customer

    Submitted Jan 09 2012

    Answer

    Thank you for contacting us.

    Lab has confirmed that they have tested this antibody in western blotting and Immunohistochemistry only - no further tests other than these has been done. I am sorry the data we have is already published on the datasheet - we are unable to provide any more tested data.

    I hope this information is nevertheless helpful to you. Please do not hesitate to contact us if you need any more advice or information.

    Read More

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