Key features and details
- Rabbit polyclonal to TEM4
- Suitable for: ICC/IF, WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Product nameAnti-TEM4 antibody
DescriptionRabbit polyclonal to TEM4
Tested applicationsSuitable for: ICC/IF, WB, IHC-Pmore details
Species reactivityReacts with: Human
An 18 amino acid synthetic peptide from near the center of human TEM4.
- Jurkat cell lysate. Human spleen tissue.
Storage instructionsShipped at 4°C. Store at +4°C.
Storage bufferPreservative: 0.02% Sodium azide
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab67278 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use a concentration of 20 µg/ml.|
|WB||Use a concentration of 1 - 2 µg/ml. Detects a band of approximately 200 kDa (predicted molecular weight: 222 kDa).|
|IHC-P||Use at an assay dependent concentration.|
RelevanceTEM4 (Tumor endothelial marker 4) is a Rho-specific GEF (guanine-nucleotide exchange factor) with novel structural and regulatory properties and predominant expression in the heart. Rho GTPases, which are activated by specific GEFs, play pivotal roles in several cellular functions. TEM4, encoding a protein containing 1510 amino acids, contains a RhoGEF-specific Dbl homology (DH) domain but lacks their typical pleckstrin homology domain. TEM4 couples tyrosine kinase signals with the activation of the rho/rac GTPases, thus leading to cell differentiation and/or proliferation. Elevated levels of TEM4, TEM5, TEM6, TEM7 and TEM7R were also raised in breast cancer tissues. TEM4 could also prove to be useful as a target, therapeutically.
- 164 kDa Rho specific guanine nucleotide exchange factor antibody
- ARHGEF 17 antibody
- ARHGEF17 antibody
Lane 1 : Anti-TEM4 antibody (ab67278) at 1 µg/ml
Lane 2 : Anti-TEM4 antibody (ab67278) at 2 µg/ml
All lanes : Jurkat cell lysate
Lysates/proteins at 15 µg per lane.
Predicted band size: 222 kDa
Observed band size: 200 kDa why is the actual band size different from the predicted?
ab67278 at 2.5 μg/ml staining of TEM4 in human spleen tissue using IHC-P.
ab67278 has been referenced in 1 publication.
- Isokane M et al. ARHGEF17 is an essential spindle assembly checkpoint factor that targets Mps1 to kinetochores. J Cell Biol 212:647-59 (2016). WB . PubMed: 26953350