Overview

  • Product name

    Anti-TEM4 antibody
  • Description

    Rabbit polyclonal to TEM4
  • Host species

    Rabbit
  • Tested applications

    Suitable for: ICC/IF, WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    An 18 amino acid synthetic peptide from near the center of human TEM4.

  • Positive control

    • Jurkat cell lysate. Human spleen tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab67278 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 20 µg/ml.
WB Use a concentration of 1 - 2 µg/ml. Detects a band of approximately 200 kDa (predicted molecular weight: 222 kDa).
IHC-P Use at an assay dependent concentration.

Target

  • Relevance

    TEM4 (Tumor endothelial marker 4) is a Rho-specific GEF (guanine-nucleotide exchange factor) with novel structural and regulatory properties and predominant expression in the heart. Rho GTPases, which are activated by specific GEFs, play pivotal roles in several cellular functions. TEM4, encoding a protein containing 1510 amino acids, contains a RhoGEF-specific Dbl homology (DH) domain but lacks their typical pleckstrin homology domain. TEM4 couples tyrosine kinase signals with the activation of the rho/rac GTPases, thus leading to cell differentiation and/or proliferation. Elevated levels of TEM4, TEM5, TEM6, TEM7 and TEM7R were also raised in breast cancer tissues. TEM4 could also prove to be useful as a target, therapeutically.
  • Database links

  • Alternative names

    • 164 kDa Rho specific guanine nucleotide exchange factor antibody
    • ARHGEF 17 antibody
    • ARHGEF17 antibody
    • FLJ90019 antibody
    • KIAA0337 antibody
    • p164 RhoGEF antibody
    • P164RHOGEF antibody
    • Rho guanine nucleotide exchange factor (GEF) 17 antibody
    • Rho guanine nucleotide exchange factor 17 antibody
    • Rho specific guanine nucleotide exchange factor 164 kDa antibody
    • TEM 4 antibody
    • Tumor endothelial marker 4 antibody
    see all

Images

  • Lane 1 : Anti-TEM4 antibody (ab67278) at 1 µg/ml
    Lane 2 : Anti-TEM4 antibody (ab67278) at 2 µg/ml

    All lanes : Jurkat cell lysate

    Lysates/proteins at 15 µg per lane.

    Predicted band size: 222 kDa
    Observed band size: 200 kDa
    why is the actual band size different from the predicted?

  • ab67278 at 2.5 μg/ml staining of TEM4 in human spleen tissue using IHC-P.

References

This product has been referenced in:

  • Isokane M  et al. ARHGEF17 is an essential spindle assembly checkpoint factor that targets Mps1 to kinetochores. J Cell Biol 212:647-59 (2016). WB . Read more (PubMed: 26953350) »
See 1 Publication for this product

Customer reviews and Q&As

Answer

Thank you for your call today and for your questions. I will let you know once I have more information about the homology of the ab67278 immunogen to the murine protein. Please see below for the discount codes to test ab121972 and ab5911 in mouse, and please let me know if you have any questions or if there is anything else that I can do for you.

DISCOUNT CODES:

Ab121972: ***
Ab5911: ***

Expiration date: July 15, 2012

These codes will each give you 1 free primary antibodybefore the expiration date. To redeem this offer, please submit an Abreview for each antibody in mouse samples,and include the code in the “Additional Comments” section of the corresponding Abreview. For more information on how to submit an Abreview, please visit the site: www.abcam.com/Abreviews.

Eachcode will be active once the Abreview has been submitted and can be redeemed in one of the following ways: 1) Call to place your order and mention the code to our customer service department; 2) Include the code in your fax order; 3) Place your order on the web and enter the promotional code.

Any feedback that you can provide will be greatly appreciated, whether positive or negative. If you have any further questions, please do not hesitate to contact us. We look forward to receiving your Abreview and wish you luck with your research.

The terms and conditions applicable to this offer can be found here: www.abcam.com/collaborationdiscount.

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