• Product name
  • Description
    Rabbit polyclonal to TEM8
  • Host species
  • Specificity
    ab21270 will recognize all three isoforms of TEM8.
  • Tested applications
    Suitable for: Flow Cyt, ICC/IF, WB, IP, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide corresponding to 13 amino acids near the center of TEM8 (Human).



Our Abpromise guarantee covers the use of ab21270 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use at an assay dependent concentration.

See Abreview.

ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.



ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.


ICC/IF Use a concentration of 20 µg/ml.
WB Use a concentration of 0.5 - 1 µg/ml. Predicted molecular weight: 45 kDa.
IP Use at an assay dependent concentration.
IHC-P Use at an assay dependent concentration.


  • Function
    Plays a role in cell attachment and migration. Interacts with extracellular matrix proteins and with the actin cytoskeleton. Mediates adhesion of cells to type 1 collagen and gelatin, reorganization of the actin cytoskeleton and promotes cell spreading. Plays a role in the angiogenic response of cultured umbilical vein endothelial cells.
  • Tissue specificity
    Detected in umbilical vein endothelial cells (at protein level). Highly expressed in tumor endothelial cells.
  • Involvement in disease
    Defects in ANTXR1 are associated with susceptibility to hemangioma capillary infantile (HCI) [MIM:602089]. HCI are benign, highly proliferative lesions involving aberrant localized growth of capillary endothelium. They are the most common tumor of infancy, occurring in up to 10% of all births. Hemangiomas tend to appear shortly after birth and show rapid neonatal growth for up to 12 months characterized by endothelial hypercellularity and increased numbers of mast cells. This phase is followed by slow involution at a rate of about 10% per year and replacement by fibrofatty stroma.
  • Sequence similarities
    Belongs to the ATR family.
    Contains 1 VWFA domain.
  • Domain
    Binding to PA occurs through the VWA domain.
  • Cellular localization
    Cell membrane. Cell projection > lamellipodium membrane. Cell projection > filopodium membrane. At the membrane of lamellipodia and at the tip of actin-enriched filopodia. Colocalizes with actin at the base of lamellipodia.
  • Information by UniProt
  • Database links
  • Alternative names
    • Anthrax toxin receptor 1 antibody
    • ANTR1_HUMAN antibody
    • Antxr1 antibody
    • ATR antibody
    • Tumor endothelial marker 8 antibody
    see all


  • Western blot analysis of TEM8 in HepG2 cell lysates with ab21270 at (A) 0.5, (B) 1 and (C) 2µg/ml. Western blot analysis of TEM8 in HepG2 cell lysates with ab21270 at (A) 0.5, (B) 1 and (C) 2µg/ml.
  • TEM8 was immunoprecipitated using 0.5mg HepG2 whole cell extract, 5µg of Rabbit polyclonal to TEM8 and 50µl of protein G magnetic beads (+). No antibody was added to the control (-).
    The antibody was incubated under agitation with Protein G beads for 10min, HepG2 whole cell extract lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.
    Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70oC; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab21270.
    Secondary: Mouse monoclonal [SB62a] Secondary Antibody to Rabbit IgG light chain (HRP) (ab99697).
    Band: Bands: 49kDa; TEM8.
  • ab21270 at 2µg/ml staining TEM8 in human brain tissue by IHC 


This product has been referenced in:
  • Jin G  et al. Combination curcumin and (-)-epigallocatechin-3-gallate inhibits colorectal carcinoma microenvironment-induced angiogenesis by JAK/STAT3/IL-8 pathway. Oncogenesis 6:e384 (2017). WB ; Human . Read more (PubMed: 28967875) »
  • Schneck H  et al. EpCAM-Independent Enrichment of Circulating Tumor Cells in Metastatic Breast Cancer. PLoS One 10:e0144535 (2015). Read more (PubMed: 26695635) »

See all 7 Publications for this product

Customer reviews and Q&As

Thank you for contacting us. We have not tested ab21270 for cross-reactivity with CMG2, but it would not be expected to react due to 23% sequence homology with the immunogen. I hope this information is helpful to you. Please do not hesitate to...

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Thank you for your reply and providing more information and a whole blot image. Your problem puzzled me greatly and I also asked other colleagues to help understand what might be happening. Here are a few of our thoughts: -For the western b...

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I'm sorry to hear you are experiencing unsatisfactory results with ab19387. I would be happy to consider offering you a replacement vial of ab21270, however before I can do that I would like to investigate this matter further to make sure that the ...

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Thank you for your enquiry. Based on prediction software, the epitope for the anti-TEM8 is extracellular. I hope this information helps. Please do not hesitate to contact us again if you need anything further.

Thank you for your enquiry. The peptide was not selected from the transmembrane domain of TEM8. Unfortunately the exact sequence of the immunogen is proprietary. Please let me know if you have any more questions.

Thank you for your enquiry. I am looking into the details of the immunogen for you. Meanwhile, I can tell you that this antibody recognizes all three isoforms of the TEM8 protein. As soon as I have any further information for you regarding the i...

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