Overview

  • Product name

    Anti-TEM8/ATR antibody [200C1339]
    See all TEM8/ATR primary antibodies
  • Description

    Mouse monoclonal [200C1339] to TEM8/ATR
  • Host species

    Mouse
  • Tested applications

    Suitable for: WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide corresponding to Human TEM8/ATR aa 481-497.
    Sequence:

    CINFTRVKNNQPAKYPL

  • Positive control

    • Tested with TEM8/ATR transfected cell lysate.
  • General notes

     This product was previously labelled as TEM8

     

Properties

Applications

Our Abpromise guarantee covers the use of ab13798 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 - 3 µg/ml. Detects a band of approximately 84 kDa.

A 70 kD band was seen when cell extracts were treated with a glycosidase cocktail. Not tested in other applications. Optimal dilutions/concentrations should be determined by the end user.

IHC-P 1/200 - 1/400. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Function

    Plays a role in cell attachment and migration. Interacts with extracellular matrix proteins and with the actin cytoskeleton. Mediates adhesion of cells to type 1 collagen and gelatin, reorganization of the actin cytoskeleton and promotes cell spreading. Plays a role in the angiogenic response of cultured umbilical vein endothelial cells.
  • Tissue specificity

    Detected in umbilical vein endothelial cells (at protein level). Highly expressed in tumor endothelial cells.
  • Involvement in disease

    Defects in ANTXR1 are associated with susceptibility to hemangioma capillary infantile (HCI) [MIM:602089]. HCI are benign, highly proliferative lesions involving aberrant localized growth of capillary endothelium. They are the most common tumor of infancy, occurring in up to 10% of all births. Hemangiomas tend to appear shortly after birth and show rapid neonatal growth for up to 12 months characterized by endothelial hypercellularity and increased numbers of mast cells. This phase is followed by slow involution at a rate of about 10% per year and replacement by fibrofatty stroma.
  • Sequence similarities

    Belongs to the ATR family.
    Contains 1 VWFA domain.
  • Domain

    Binding to PA occurs through the VWA domain.
  • Cellular localization

    Cell membrane. Cell projection > lamellipodium membrane. Cell projection > filopodium membrane. At the membrane of lamellipodia and at the tip of actin-enriched filopodia. Colocalizes with actin at the base of lamellipodia.
  • Information by UniProt
  • Database links

  • Alternative names

    • Anthrax toxin receptor 1 antibody
    • ANTR1_HUMAN antibody
    • Antxr1 antibody
    • ATR antibody
    • Tumor endothelial marker 8 antibody
    see all

Images

  • Anti-TEM8/ATR antibody [200C1339] (ab13798) at 1 µg + TEM8/ATR transfected cell lysate
  • Formalin-fixed, paraffin-embedded human colon stained with ab13798 diluted 1:200, detection using peroxidase-conjugated secondary antibody and DAB chromogen.
  • Formalin-fixed, paraffin-embedded human colon stained with ab13798 diluted 1:200, detection using peroxidase-conjugated secondary and DAB chromogen.

References

This product has been referenced in:

  • Sakai Y  et al. Bcl-2 mediated modulation of vascularization in prostate cancer xenografts. Prostate 69:459-70 (2009). WB ; Human . Read more (PubMed: 19107861) »
See 1 Publication for this product

Customer reviews and Q&As

Application
Western blot
Sample
Dog Cell lysate - whole cell (Hemangiosarcoma cell lines)
Gel Running Conditions
Reduced Denaturing (4-20%)
Loading amount
50 µg
Specification
Hemangiosarcoma cell lines
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 24°C

Holly Pondenis

Verified customer

Submitted Feb 20 2017

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