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  1. Link

    tem8atr-antibody-ab21270.pdf

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Tags & Cell Markers Cell Type Markers Tumor Associated
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Anti-TEM8/ATR antibody (ab21270)

  • Datasheet
  • SDS
Reviews (2)Q&A (6)References (10)

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Western blot - Anti-TEM8/ATR antibody (ab21270)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TEM8/ATR antibody (ab21270)

Key features and details

  • Rabbit polyclonal to TEM8/ATR
  • Suitable for: WB, IHC-P
  • Reacts with: Human
  • Isotype: IgG

You may also be interested in

Protein
Product image
Recombinant Human TEM8/ATR protein (ab132123)
Secondary
Product image
Goat Anti-Rabbit IgG H&L (HRP) (ab205718)

View more associated products

Overview

  • Product name

    Anti-TEM8/ATR antibody
    See all TEM8/ATR primary antibodies
  • Description

    Rabbit polyclonal to TEM8/ATR
  • Host species

    Rabbit
  • Specificity

    ab21270 will recognize all three isoforms of TEM8/ATR. The immunogen used for this product shares 100% homology with mouse and rat TEM8. However, thus far cross-reactivity with these species has not been confirmed experimentally.

  • Tested applications

    Suitable for: WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide corresponding to Human TEM8/ATR aa 220-270 (internal sequence).

  • General notes

    This product was previously labelled as TEM8.

    Some of our customers have successfully used ab21270 in Flow Cytometry, please see PubMed ID: 24742682, 21414394 and 18723498. However, we have not tested ab21270 in this application and therefore we cannot guarantee that it will consistently work in Flow.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.2
    Preservative: 0.02% Sodium azide
    Constituent: PBS
  • Concentration information loading...
  • Purification notes

    Ion exchange chromatography.
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Tags & Cell Markers
    • Cell Type Markers
    • Tumor Associated
    • Microbiology
    • Protein
    • Human Protein
    • Defensin
    • Tags & Cell Markers
    • Cell Type Markers
    • Epi / Endo-thelial
    • Cardiovascular
    • Angiogenesis
    • Angiogenic Factors
    • Cardiovascular
    • Angiogenesis
    • Endothelial Cell Markers

Associated products

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Conjugation kits

    • PE / R-Phycoerythrin Conjugation Kit - Lightning-Link® (ab102918)
    • APC Conjugation Kit - Lightning-Link® (ab201807)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Recombinant Protein

    • Recombinant Human TEM8/ATR protein (ab132123)

Applications

Our Abpromise guarantee covers the use of ab21270 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.5 - 1 µg/ml. Predicted molecular weight: 45 kDa.
IHC-P Use at an assay dependent concentration.

Target

  • Function

    Plays a role in cell attachment and migration. Interacts with extracellular matrix proteins and with the actin cytoskeleton. Mediates adhesion of cells to type 1 collagen and gelatin, reorganization of the actin cytoskeleton and promotes cell spreading. Plays a role in the angiogenic response of cultured umbilical vein endothelial cells.
  • Tissue specificity

    Detected in umbilical vein endothelial cells (at protein level). Highly expressed in tumor endothelial cells.
  • Involvement in disease

    Defects in ANTXR1 are associated with susceptibility to hemangioma capillary infantile (HCI) [MIM:602089]. HCI are benign, highly proliferative lesions involving aberrant localized growth of capillary endothelium. They are the most common tumor of infancy, occurring in up to 10% of all births. Hemangiomas tend to appear shortly after birth and show rapid neonatal growth for up to 12 months characterized by endothelial hypercellularity and increased numbers of mast cells. This phase is followed by slow involution at a rate of about 10% per year and replacement by fibrofatty stroma.
  • Sequence similarities

    Belongs to the ATR family.
    Contains 1 VWFA domain.
  • Domain

    Binding to PA occurs through the VWA domain.
  • Cellular localization

    Cell membrane. Cell projection > lamellipodium membrane. Cell projection > filopodium membrane. At the membrane of lamellipodia and at the tip of actin-enriched filopodia. Colocalizes with actin at the base of lamellipodia.
  • Target information above from: UniProt accession Q9H6X2 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 84168 Human
    • Omim: 606410 Human
    • SwissProt: Q9H6X2 Human
    • Unigene: 165859 Human
    • Alternative names

      • Anthrax toxin receptor 1 antibody
      • ANTR1_HUMAN antibody
      • Antxr1 antibody
      • ATR antibody
      • Tumor endothelial marker 8 antibody
      see all

    Images

    • Western blot - Anti-TEM8/ATR antibody (ab21270)
      Western blot - Anti-TEM8/ATR antibody (ab21270)

      Western blot analysis of TEM8/ATR in HepG2 cell lysates with ab21270 at (A) 0.5, (B) 1 and (C) 2µg/ml. Western blot analysis of TEM8/ATR in HepG2 cell lysates with ab21270 at (A) 0.5, (B) 1 and (C) 2µg/ml.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TEM8/ATR antibody (ab21270)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TEM8/ATR antibody (ab21270)

      ab21270 at 2µg/ml staining TEM8/ATR in human brain tissue by IHC 

    Protocols

    • Flow cytometry protocols
    • Immunohistochemistry protocols
    • Immunocytochemistry & immunofluorescence protocols
    • Western blot protocols

    Click here to view the general protocols

    Datasheets and documents

    • Datasheet
    • SDS
  • References (10)

    Publishing research using ab21270? Please let us know so that we can cite the reference in this datasheet.

    ab21270 has been referenced in 10 publications.

    • Alcalá S  et al. The Anthrax Toxin Receptor 1 (ANTXR1) Is Enriched in Pancreatic Cancer Stem Cells Derived from Primary Tumor Cultures. Stem Cells Int 2019:1378639 (2019). PubMed: 31191663
    • Høye AM  et al. Tumor endothelial marker 8 promotes cancer progression and metastasis. Oncotarget 9:30173-30188 (2018). PubMed: 30046396
    • Gong Q  et al. Effect of silencing TEM8 gene on proliferation, apoptosis, migration and invasion of XWLC-05 lung cancer cells. Mol Med Rep 17:911-917 (2018). PubMed: 29115620
    • Jin G  et al. Combination curcumin and (-)-epigallocatechin-3-gallate inhibits colorectal carcinoma microenvironment-induced angiogenesis by JAK/STAT3/IL-8 pathway. Oncogenesis 6:e384 (2017). WB ; Human . PubMed: 28967875
    • Schneck H  et al. EpCAM-Independent Enrichment of Circulating Tumor Cells in Metastatic Breast Cancer. PLoS One 10:e0144535 (2015). PubMed: 26695635
    • Arévalo MT  et al. Targeted Silencing of Anthrax Toxin Receptors Protects against Anthrax Toxins. J Biol Chem 289:15730-15738 (2014). Flow Cyt ; Human . PubMed: 24742682
    • Langer M  et al. Bacillus anthracis lethal toxin reduces human alveolar epithelial barrier function. Infect Immun 80:4374-87 (2012). Human . PubMed: 23027535
    • Kibria G  et al. A new peptide motif present in the protective antigen of anthrax toxin exerts its efficiency on the cellular uptake of liposomes and applications for a dual-ligand system. Int J Pharm 412:106-14 (2011). Flow Cyt ; Human . PubMed: 21414394
    • Wu W  et al. Resistance of Human Alveolar Macrophages to Bacillus anthracis Lethal Toxin. J Immunol 183:5799-806 (2009). WB ; Human, Mouse . PubMed: 19812208
    • Bagley RG  et al. Human endothelial precursor cells express tumor endothelial marker 1/endosialin/CD248. Mol Cancer Ther 7:2536-46 (2008). Flow Cyt ; Human . PubMed: 18723498

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    1-8 of 8 Abreviews or Q&A

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-TEM8/ATR antibody

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Dog Tissue sections (Canine prostate)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: 0.01M Citrate buffer pH6 65'c 60min.
    Permeabilization
    No
    Specification
    Canine prostate
    Blocking step
    Block Ace as blocking agent for 30 minute(s) · Concentration: 4% · Temperature: 25°C
    Fixative
    Paraformaldehyde
    Read More

    Abcam user community

    Verified customer

    Submitted May 22 2017

    Question

    Will this TEM8 antibody cross-react with CMG2 Receptor?

    Read More

    Abcam community

    Verified customer

    Asked on Jun 13 2011

    Answer

    Thank you for contacting us. We have not tested ab21270 for cross-reactivity with CMG2, but it would not be expected to react due to 23% sequence homology with the immunogen. I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

    Read More

    Abcam Scientific Support

    Answered on Jun 13 2011

    Question

    find attached a word document with answers. My results do not conclude anything about mouse. I am already trying to understand what might be happening and would be very grateful if you can clarify a few points: -there are several bands on all blots, I'd be very interrested to know their sizes but with only one MW marker am finding it hard to determine this (are the bands above 78kda close to 100kDa for example, or 80kDa?) These are criterion gradient gels from 5 to 15%. I’m not sure which bands you refer to. I’m including at the end of the file a scan with the complete gel FYI. -Looking at the western blots of the lysates, I see one band with the Mono (I guess this is the 80Kda band), but two bands both with the poly and the HA tag antibodies. I am wondering if the HA tag is not also a poly. In general polys do pick up better various forms of the proteins and it could be that those two polys are picking up cleaved forms of TEM8. Could this be the case? In the case of the poly, there are many bands but I can’t comment because none of them looks like the ones recognized by HA or the mono when you compare the MW or the shape. The lower MW band picked up by HA is not specific since it is found in the control lysate too. The band for TEM8 is seen as a doublet because it is a re-probing after using the monoclonal. When you strip blots, you take off the secondary antibody but not the primary, so you see it as an “empty space”. The HA tag antibody is a rat monoclonal and recognizes a tag in the C-terminus of the protein. -Looking at the IPed reprobes, it seems again to me that the poly anti TEM8 and the HA tags are giving similar band patterns, wether it was an IP with HA antibody or the poly. So with the IP with the poly it seems to me that the reprobe with the HA does give something, and as the TEM8 is expressed not endogenously in your cells I am wondering if again it could be because of a cleavage of the protein. Interrestingly IPing with the mono did not give a band at 80kda, so I conclude that the mono does not work well in IP, is this your opinion too? Yes, the mono is good only in western blots. The Ipps suggests that the Abcam antibody does IP Tem8 but what concerns me is that when you IP with Abcam and probe again with Abcam, you don’t see the expected band. I don’t think that it is a problem with Abcam Ab working in Western blots because it recognizes the expected band when Iped with anti HA. I don’t know what are the bands right below but I think it is unspecific because you see it in all 3 lanes with ipps and even in the lysates. I would be very grateful if you can let me know your thoughts on those points so I can better understand what might be happening in your experiments. The MW markers are: 200, 130, 78, 39, 32, 17, 7.

    Read More

    Abcam community

    Verified customer

    Asked on Nov 14 2006

    Answer

    Thank you for your reply and providing more information and a whole blot image. Your problem puzzled me greatly and I also asked other colleagues to help understand what might be happening. Here are a few of our thoughts: -For the western blots: you said "The band for TEM8 is seen as a doublet because it is a re-probing after using the monoclonal. When you strip blots, you take off the secondary antibody but not the primary, so you see it as an “empty space”." Have you tried using the antibody on a fresh membrane? Stripping can damage the proteins and therefore prevent them from being detected by the second primary antibody. Furthermore if there is still primary antibody from the first experiment the second primary antibody will have more problems binding to the protein. -The Abcam antibody has not been tested on human samples, and as the protein is expressed in different isoforms/ splice variants we are wondering if the Abcam antibody is better at detecting one splice variant over an other, hence in our samples of mouse kidney it can detect the protein well in the three isoforms while in your samples the expression pattern may be very different. We would recommend to run a positive control of mouse kidney whole cell lysate to determine if this is the case. For the Immunoprecipitations: -The small strong large band in the IPed samples (HA and Poly IPed) and reprobed with the polyclonals could be the 60kDa form of the protein, and the strong large band could be the 45kDa form of the protein. As the poly has not been tested in IP, it's very hard to know if it is working optimally for this application. Again, having a fresh membrane would be useful to see exactly what bands are due to what antibody. As the antibody has not been tested in human samples I think the best recommendation I can make is to run a positive control along your samples; we do not guarantee ab19387 to work in human but I would be more than happy to offer you a refund or another antibody if you still have a problem with the positive control.

    Read More

    Abcam Scientific Support

    Answered on Nov 15 2006

    Question

    In September, I purchased the antibody cat# 19387 against TEM8 to be able to identify the 3 molecular forms of TEM8. I preferred this antibody over the other options offered in your catalog because there is a publication with this antibody and it comes from a lab with a good reputation. However, my experience with it was disappointing: It was prepared with a sequence of human TEM8, consequently it should recognize human TEM8. I tested it in Western blot and immunoprecipitation with cells expressing human TEM8 cDNA (see JBC 281:23227) and compared it to an antibody against the tag and a commercial antibody from another source. I'm attaching the results for your inspection. The last panel is a reprobing after the monoclonal antibody, therefore a blank band is observed in the samples with the highest levels of TEM8. As you can see, used in western blot, it is very unspecific and it does not recognize TEM8 as the main band in a lysate. It also doesn't work well in immunoprecipitation. Would it be possible for me to send this antibody back and try catalog # 21270, which also could potentially meet the features I'm interested in ?

    Read More

    Abcam community

    Verified customer

    Asked on Nov 13 2006

    Answer

    I'm sorry to hear you are experiencing unsatisfactory results with ab19387. I would be happy to consider offering you a replacement vial of ab21270, however before I can do that I would like to investigate this matter further to make sure that the problem with ab19387 could not be solved with a few protocol modifications. The quality of our products is of utmost importance to us and should we find out that ab19387 is of poor quality we will not hesitate to remove it from the catalogue. Can you please click on the link below which will take you to a short protocol questionnaire to help you put the information together, and could you please put it to my attention so I can personally look into this matter as I already have in hand the images you kindly provided. https://www.abcam.com/index.html?section=ip&pageconfig=technical&intAbID=19387&mode=questionaire I am already trying to understand what might be happening and would be very grateful if you can clarify a few points: -there are several bands on all blots, I'd be very interrested to know their sizes but with only one MW marker am finding it hard to determine this (are the bands above 78kda close to 100kDa for example, or 80kDa?) -Looking at the western blots of the lysates, I see one band with the Mono (I guess this is the 80Kda band), but two bands both with the poly and the HA tag antibodies. I am wondering if the HA tag is not also a poly. In general polys do pick up better various forms of the proteins and it could be that those two polys are picking up cleaved forms of TEM8. Could this be the case? -Looking at the IPed reprobes, it seems again to me that the poly anti TEM8 and the HA tags are giving similar band patterns, wether it was an IP with HA antibody or the poly. So with the IP with the poly it seems to me that the reprobe with the HA does give something, and as the TEM8 is expressed not endogenously in your cells I am wondering if again it could be because of a cleavage of the protein. Interrestingly IPing with the mono did not give a band at 80kda, so I conclude that the mono does not work well in IP, is this your opinion too? I would be very grateful if you can let me know your thoughts on those points so I can better understand what might be happening in your experiments.

    Read More

    Abcam Scientific Support

    Answered on Nov 14 2006

    Question

    Hi Karen, Was it (peptide antigen for TEM-8) from the extracellular domain? That is all I need to know.

    Read More

    Abcam community

    Verified customer

    Asked on Mar 07 2006

    Answer

    Thank you for your enquiry. Based on prediction software, the epitope for the anti-TEM8 is extracellular. I hope this information helps. Please do not hesitate to contact us again if you need anything further.

    Read More

    Abcam Scientific Support

    Answered on Mar 08 2006

    Question

    I would like to know the details of the amino acid sequence / epitope against which ab21270 is raised. Is this epitope in the transmembrane domain of TEM8? Does it recognize the short, mid and long forms of TEM8 generated from alternate splicing? Thanks

    Read More

    Abcam community

    Verified customer

    Asked on Mar 07 2006

    Answer

    Thank you for your enquiry. The peptide was not selected from the transmembrane domain of TEM8. Unfortunately the exact sequence of the immunogen is proprietary. Please let me know if you have any more questions.

    Read More

    Abcam Scientific Support

    Answered on Mar 07 2006

    Question

    I would like to know the details of the amino acid sequence / epitope against which ab21270 is raised. Is this epitope in the transmembrane domain of TEM8? Does it recognize the short, mid and long forms of TEM8 generated from alternate splicing? Thanks

    Read More

    Abcam community

    Verified customer

    Asked on Mar 07 2006

    Answer

    Thank you for your enquiry. I am looking into the details of the immunogen for you. Meanwhile, I can tell you that this antibody recognizes all three isoforms of the TEM8 protein. As soon as I have any further information for you regarding the immunogen, I will let you know. I hope this information helps, please do not hesitate to contact us if you need any more advice or information.

    Read More

    Abcam Scientific Support

    Answered on Mar 07 2006

    Flow Cytometry abreview for Anti-TEM8 antibody

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Flow Cytometry
    Sample
    Human Cell (pericytes)
    Specification
    pericytes
    Permeabilization
    No
    Read More

    Abcam user community

    Verified customer

    Submitted Oct 28 2005

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