Overview

  • Product name
  • Description
    Rabbit polyclonal to TEM8/ATR
  • Host species
    Rabbit
  • Specificity

    ab21270 will recognize all three isoforms of TEM8/ATR.

  • Tested applications
    Suitable for: Flow Cyt, ICC/IF, WB, IP, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide corresponding to Human TEM8/ATR (internal sequence).

  • Positive control
    • HepG2 whole cell lysate (ab7900) can be used as a positive control in WB.
  • General notes

     This product was previously labelled as TEM8

     

Properties

Applications

Our Abpromise guarantee covers the use of ab21270 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use at an assay dependent concentration.

See Abreview.

ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.

 

 

ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.

 

ICC/IF Use a concentration of 20 µg/ml.
WB Use a concentration of 0.5 - 1 µg/ml. Predicted molecular weight: 45 kDa.
IP Use at an assay dependent concentration.
IHC-P Use at an assay dependent concentration.

Target

  • Function
    Plays a role in cell attachment and migration. Interacts with extracellular matrix proteins and with the actin cytoskeleton. Mediates adhesion of cells to type 1 collagen and gelatin, reorganization of the actin cytoskeleton and promotes cell spreading. Plays a role in the angiogenic response of cultured umbilical vein endothelial cells.
  • Tissue specificity
    Detected in umbilical vein endothelial cells (at protein level). Highly expressed in tumor endothelial cells.
  • Involvement in disease
    Defects in ANTXR1 are associated with susceptibility to hemangioma capillary infantile (HCI) [MIM:602089]. HCI are benign, highly proliferative lesions involving aberrant localized growth of capillary endothelium. They are the most common tumor of infancy, occurring in up to 10% of all births. Hemangiomas tend to appear shortly after birth and show rapid neonatal growth for up to 12 months characterized by endothelial hypercellularity and increased numbers of mast cells. This phase is followed by slow involution at a rate of about 10% per year and replacement by fibrofatty stroma.
  • Sequence similarities
    Belongs to the ATR family.
    Contains 1 VWFA domain.
  • Domain
    Binding to PA occurs through the VWA domain.
  • Cellular localization
    Cell membrane. Cell projection > lamellipodium membrane. Cell projection > filopodium membrane. At the membrane of lamellipodia and at the tip of actin-enriched filopodia. Colocalizes with actin at the base of lamellipodia.
  • Information by UniProt
  • Database links
  • Alternative names
    • Anthrax toxin receptor 1 antibody
    • ANTR1_HUMAN antibody
    • Antxr1 antibody
    • ATR antibody
    • Tumor endothelial marker 8 antibody
    see all

Images

  • Western blot analysis of TEM8/ATR in HepG2 cell lysates with ab21270 at (A) 0.5, (B) 1 and (C) 2µg/ml. Western blot analysis of TEM8/ATR in HepG2 cell lysates with ab21270 at (A) 0.5, (B) 1 and (C) 2µg/ml.

  • TEM8/ATR was immunoprecipitated using 0.5mg HepG2 whole cell extract, 5µg of Rabbit polyclonal to TEM8/ATR and 50µl of protein G magnetic beads (+). No antibody was added to the control (-).
    The antibody was incubated under agitation with Protein G beads for 10min, HepG2 whole cell extract lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.
    Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70oC; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab21270.
    Secondary: Mouse monoclonal [SB62a] Secondary Antibody to Rabbit IgG light chain (HRP) (ab99697).
    Band: Bands: 49kDa; TEM8/ATR.

  • ab21270 at 2µg/ml staining TEM8/ATR in human brain tissue by IHC 

References

This product has been referenced in:
  • Høye AM  et al. Tumor endothelial marker 8 promotes cancer progression and metastasis. Oncotarget 9:30173-30188 (2018). Read more (PubMed: 30046396) »
  • Gong Q  et al. Effect of silencing TEM8 gene on proliferation, apoptosis, migration and invasion of XWLC-05 lung cancer cells. Mol Med Rep 17:911-917 (2018). Read more (PubMed: 29115620) »
See all 9 Publications for this product

Customer reviews and Q&As

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1-2 of 2 Abreviews

Abcam has not validated the combination of species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Dog Tissue sections (Canine prostate)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: 0.01M Citrate buffer pH6 65'c 60min.
Permeabilization
No
Specification
Canine prostate
Blocking step
Block Ace as blocking agent for 30 minute(s) · Concentration: 4% · Temperature: 25°C
Fixative
Paraformaldehyde

Abcam user community

Verified customer

Submitted May 22 2017

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Flow Cytometry
Sample
Human Cell (pericytes)
Specification
pericytes
Permeabilization
No

Abcam user community

Verified customer

Submitted Oct 28 2005

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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