Recombinant Anti-TEMT antibody [EPR13240-105] - BSA and Azide free (ab250773)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR13240-105] to TEMT - BSA and Azide free
- Suitable for: WB
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-TEMT antibody [EPR13240-105] - BSA and Azide free
See all TEMT primary antibodies -
Description
Rabbit monoclonal [EPR13240-105] to TEMT - BSA and Azide free -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat -
Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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General notes
ab250773 is the carrier-free version of ab184573.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR13240-105 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Conjugation kits
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab250773 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
Use at an assay dependent concentration. Detects a band of approximately 29 kDa (predicted molecular weight: 29 kDa).
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Notes |
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WB
Use at an assay dependent concentration. Detects a band of approximately 29 kDa (predicted molecular weight: 29 kDa). |
Target
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Function
Catalyzes the N-methylation of tryptamine and structurally related compounds. -
Tissue specificity
Widely expressed. The highest levels were in thyroid, adrenal gland, adult and fetal lung. Intermediate levels in heart, placenta, skeletal muscle, testis, small intestine, pancreas, stomach, spinal cord, lymph node and trachea. Very low levels in adult and fetal kidney and liver, in adult spleen, thymus, ovary, colon and bone marrow. Not expressed in peripheral blood leukocytes and brain. -
Sequence similarities
Belongs to the NNMT/PNMT/TEMT family. -
Cellular localization
Cytoplasm. - Information by UniProt
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Database links
- Entrez Gene: 11185 Human
- Entrez Gene: 21743 Mouse
- Entrez Gene: 368066 Rat
- Omim: 604854 Human
- SwissProt: O95050 Human
- SwissProt: P40936 Mouse
- Unigene: 632629 Human
- Unigene: 299 Mouse
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Alternative names
- Amine N methyltransferase antibody
- Amine N-methyltransferase antibody
- Aromatic alkylamine N methyltransferase antibody
see all
Images
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All lanes : Anti-TEMT antibody [EPR13240-105] (ab184573) at 1/1000 dilution
Lane 1 : Human adrenal gland lysate
Lane 2 : Human fetal liver lysate
Lane 3 : Human fetal lung lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution
Predicted band size: 29 kDa
Observed band size: 29 kDaThis data was developed using ab184573, the same antibody clone in a different buffer formulation.
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Anti-TEMT antibody [EPR13240-105] (ab184573) at 1/1000 dilution + A549 cell lysate at 20 µg
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution
Predicted band size: 29 kDa
Observed band size: 29 kDaThis data was developed using ab184573, the same antibody clone in a different buffer formulation.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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Datasheet download
Certificate of Compliance
References (0)
ab250773 has not yet been referenced specifically in any publications.