Overview

  • Product name

    Anti-Tenascin C antibody [EB2]
    See all Tenascin C primary antibodies
  • Description

    Mouse monoclonal [EB2] to Tenascin C
  • Host species

    Mouse
  • Specificity

    ab88280 reacts with the 4th and 5th fibronectin repeats of human Tenascin C polypeptides.
  • Tested applications

    Suitable for: WB, IHC-Fr, ICC/IFmore details
  • Species reactivity

    Reacts with: Rabbit, Guinea pig, Human
  • Immunogen

    Purified Tenascin C from human fetal fibroblasts.

  • Positive control

    • Vulva carcinoma
  • General notes


    ab88280 is derived from the hybridoma produced by fusion between myeloma cells and Balb/c spleen cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab88280 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Predicted molecular weight: 241 kDa.
IHC-Fr Use at an assay dependent concentration.
ICC/IF 1/1000.

Target

  • Function

    Extracellular matrix protein implicated in guidance of migrating neurons as well as axons during development, synaptic plasticity as well as neuronal regeneration. Promotes neurite outgrowth from cortical neurons grown on a monolayer of astrocytes. Ligand for integrins alpha-8/beta-1, alpha-9/beta-1, alpha-V/beta-3 and alpha-V/beta-6.
  • Sequence similarities

    Belongs to the tenascin family.
    Contains 15 EGF-like domains.
    Contains 1 fibrinogen C-terminal domain.
    Contains 15 fibronectin type-III domains.
  • Cellular localization

    Secreted > extracellular space > extracellular matrix.
  • Information by UniProt
  • Database links

  • Alternative names

    • 150 225 antibody
    • Cytotactin antibody
    • Glioma associated extracellular matrix antigen antibody
    • Glioma-associated-extracellular matrix antigen antibody
    • GMEM antibody
    • GP 150 225 antibody
    • GP 150-225 antibody
    • GP antibody
    • Hexabrachion antibody
    • HXB antibody
    • JI antibody
    • MGC167029 antibody
    • Miotendinous antigen antibody
    • Myotendinous antigen antibody
    • Neuronectin antibody
    • TENA_HUMAN antibody
    • Tenascin antibody
    • Tenascin-C antibody
    • Tenascin-C isoform 14/AD1/16 antibody
    • TenascinC antibody
    • TN antibody
    • TN C antibody
    • TN-C antibody
    • TNC antibody
    see all

Images

  • ab88280, staining Tenascin C in vulva carcinoma frozen tissue sections by Immunohistochemistry.

References

This product has been referenced in:

  • Rodríguez MA  et al. Gene expression profile of extraocular muscles following resection strabismus surgery. Exp Eye Res 182:182-193 (2019). Read more (PubMed: 30953624) »
  • Canseco JA  et al. Effect on ligament marker expression by direct-contact co-culture of mesenchymal stem cells and anterior cruciate ligament cells. Tissue Eng Part A 18:2549-58 (2012). Read more (PubMed: 22780864) »
See all 3 Publications for this product

Customer reviews and Q&As

1-4 of 4 Abreviews or Q&A

Answer

Thank you for submitting an Abreview of ab88280. As you may have noticed, your review has now been published on our website.

Since you obtained poor results using the antibody in a untested application, we would like to follow up on this to see if we can possibly improve the results you are seeing with this antibody.

Based on the information in your review, I feel that altering the following step in your protocol may help improve your results:
- try an enzymatic antigen retrieval step

If you decide to follow these suggestions, please let me know if they are helpful.
I wish you good luck with your research.

Read More
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (Breast Cancer Cell Lines)
Specification
Breast Cancer Cell Lines
Fixative
Formaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: PH9/6
Permeabilization
No

Mr. Stefan Swierczynski

Verified customer

Submitted Jun 11 2012

Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (Breast Cancer Cell Line MDA-MB231)
Specification
Breast Cancer Cell Line MDA-MB231
Fixative
Methanol
Permeabilization
No

Mr. Stefan Swierczynski

Verified customer

Submitted Jun 07 2012

Answer

DISCOUNT CODE: 000000

Ablaufdatum: 21. September 2012

Es freut mich zu hören, dass Sie unser Angebot unseren Antikörper ab88280 inIHC-P zu testen, annehmen möchten. Wie am gewünscht, können Sie den Gutscheincode zum Kauf eines weiteren Antikörpers einsetzen, sobald Sie uns ein Abreview für diesen Antikörper eingereicht haben. Der Discountcode hat die Nummer 0000, deckt den Wert von ab88280 ab und kann zum Kauf weiterer Produkte eingesetzt werden.

Der Code wird gültig, sobald Sie uns ein Abreview für diesen Antikörper über den Test inIHC-P eingereicht haben. Bitte geben Sie diesen Code auch im Abschnitt “Additional Notes” des Abreviews mit an, so dass wir wissen, dass sich dieses Abreview auf die Gutscheinaktion bezieht. Der Code wird dadurch aktiviert. Bitte kontaktieren Sie bei der nächsten Bestellung unsere Kundendienst mit dem Code und der ursprünglichen Bestellnummer.

Bitte zögern Sie nicht, sich wieder an uns zu wenden, falls Sie weitere Fragen haben. Wir freuen uns auf Ihr Abreview, egal ob Ihre Ergebnisse positiv oder negativ sind und wünschen Ihnen viel Glück für Ihre Forschung.

Die Bedingungen dieses Angebotes befinden sich unter dem folgenden Link: www.abcam.com/collaborationdiscount

Read More

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