Anti-Tenascin C antibody [EB2] (ab88280)
Key features and details
- Mouse monoclonal [EB2] to Tenascin C
- Suitable for: IHC-Fr, WB
- Knockout validated
- Reacts with: Human
- Isotype: IgG1
Overview
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Product name
Anti-Tenascin C antibody [EB2]
See all Tenascin C primary antibodies -
Description
Mouse monoclonal [EB2] to Tenascin C -
Host species
Mouse -
Specificity
ab88280 reacts with the 4th and 5th fibronectin repeats of human Tenascin C polypeptides. -
Tested applications
Suitable for: IHC-Fr, WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
Purified Tenascin C from human fetal fibroblasts.
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Positive control
- Vulva carcinoma
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General notes
ab88280 is derived from the hybridoma produced by fusion between myeloma cells and Balb/c spleen cells.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
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Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.09% Sodium azide
Constituents: 1% BSA, PBS -
Concentration information loading...
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Purity
Protein G purified -
Primary antibody notes
ab88280 is derived from the hybridoma produced by fusion between myeloma cells and Balb/c spleen cells. -
Clonality
Monoclonal -
Clone number
EB2 -
Isotype
IgG1 -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab88280 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-Fr |
Use at an assay dependent concentration.
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WB |
1/1000. Predicted molecular weight: 241 kDa.
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Notes |
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IHC-Fr
Use at an assay dependent concentration. |
WB
1/1000. Predicted molecular weight: 241 kDa. |
Target
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Function
Extracellular matrix protein implicated in guidance of migrating neurons as well as axons during development, synaptic plasticity as well as neuronal regeneration. Promotes neurite outgrowth from cortical neurons grown on a monolayer of astrocytes. Ligand for integrins alpha-8/beta-1, alpha-9/beta-1, alpha-V/beta-3 and alpha-V/beta-6. -
Sequence similarities
Belongs to the tenascin family.
Contains 15 EGF-like domains.
Contains 1 fibrinogen C-terminal domain.
Contains 15 fibronectin type-III domains. -
Cellular localization
Secreted > extracellular space > extracellular matrix. - Information by UniProt
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Database links
- Entrez Gene: 3371 Human
- Omim: 187380 Human
- SwissProt: P24821 Human
- Unigene: 143250 Human
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Alternative names
- 150 225 antibody
- Cytotactin antibody
- Glioma associated extracellular matrix antigen antibody
see all
Images
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All lanes : Anti-Tenascin C antibody [EB2] (ab88280) at 1/1000 dilution
Lane 1 : Wild-type U-2 OS Treated BFA (5 ug/mL, 6 h) cell lysate
Lane 2 : TNC knockout U-2 OS Treated BFA (5 ug/mL, 6 h) cell lysate
Lane 3 : Wild-type U-2 OS Vehicle Control BFA (0 ug/mL, 6 h) cell lysate
Lane 4 : TNC knockout U-2 OS Vehicle Control BFA (0 ug/mL, 6 h) cell lysate
Lane 5 : U-87 MG Treated BFA (5ug/mL, 6h) cell lysate
Lane 6 : U-87 MG cell lysate
Lysates/proteins at 40 µg per lane.
Performed under reducing conditions.
Predicted band size: 241 kDa
Observed band size: 200,270 kDa why is the actual band size different from the predicted?False colour image of Western blot: Anti-Tenascin C antibody [EB2] staining at 1/1000 dilution, shown in green; Rabbit anti-ACTN4 [EPR2533(2)] (ab108198) loading control staining at 1/20000 dilution, shown in red. In Western blot, ab88280 was shown to bind specifically to Tenascin C. A band was observed at 200/270 kDa in treated wild-type U-2 OS cell lysates with no signal observed at this size in TNC knockout cell line ab262488 (knockout cell lysate ab263924). To generate this image, wild-type and TNC knockout U-2 OS cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 2 % BSA in TBS-0.1 % Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times before development with Optiblot (ECL reagent ab133456) and imaged with 16 minutes exposure time. Secondary antibodies used were HRP conjugated Goat anti-Mouse (H+L) and Goat anti-Rabbit IgG H&L (IRDye® 680RD) preabsorbed (ab216777) at 1/20000 dilution.
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ab88280, staining Tenascin C in vulva carcinoma frozen tissue sections by Immunohistochemistry.
Protocols
Datasheets and documents
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Datasheet download
References (5)
ab88280 has been referenced in 5 publications.
- Liu JX & Pedrosa Domellöf F Cytoskeletal Proteins in Myotendinous Junctions of Human Extraocular Muscles. Invest Ophthalmol Vis Sci 62:19 (2021). PubMed: 33595614
- Hering H et al. TatS: a novel in vitro tattooed human skin model for improved pigment toxicology research. Arch Toxicol 94:2423-2434 (2020). PubMed: 32661687
- Rodríguez MA et al. Gene expression profile of extraocular muscles following resection strabismus surgery. Exp Eye Res 182:182-193 (2019). PubMed: 30953624
- Canseco JA et al. Effect on ligament marker expression by direct-contact co-culture of mesenchymal stem cells and anterior cruciate ligament cells. Tissue Eng Part A 18:2549-58 (2012). PubMed: 22780864
- Angunawela RI et al. Refractive lenticule re-implantation after myopic ReLEx: a feasibility study of stromal restoration after refractive surgery in a rabbit model. Invest Ophthalmol Vis Sci 53:4975-85 (2012). PubMed: 22743323