Anti-Tenascin C antibody [EPR4219] (ab108930)

Immunohistochemistry (Frozen sections) analysis of Mouse E14 spinal cord labeling Tenascin C with ab108930 at 1/100 dilution (4.27μg/ml). Tissue was fixed with 4% PFA and permeabilised with 0.2% TritonX-100. Antigen retrieval was performed using a heated citrate solution (10mM citrate PH 6.0 + 0.05% Tween-20). ab150077, an AlexaFluor^® 488 Goat anti-Rabbit secondary antibody was used at 1/1000 (2 μg/ml). DAPI nuclear counter stain. Positive staining on mesenchymal condensations during chondrogenesis of mouse E14 embryo (PMID: 9822997; PMID: 19586317; PMID: 24778247).

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of rat cerebellar cortex labeling Tenascin C with ab108930 at 1/500 dilution (0.854 μg/ml). Heat mediated antigen retrieval was performed using Tris/EDTA Buffer, PH9 (ab93684). A ready to use Goat Anti-Rabbit IgG H&L (HRP) secondary antibody was used. Hematoxylin counter stain. Staining on the molecular layer of rat cerebellar cortex (PMID: 1372043).

Blocking and diluting buffer and concentration: 5% NFDM /TBST.

The molecular weight observed is consistent with what has been described in the literature (PMID: 10462531).

Immunohistochemistry (Frozen sections) analysis of Rat cerebellar cortex labeling Tenascin C with ab108930 at 1/100 dilution (4.27μg/ml). Tissue was fixed with 4% PFA and permeabilised with 0.2% TritonX-100. Antigen retrieval was performed using a heated citrate solution (10mM citrate PH 6.0 + 0.05% Tween-20). ab150077, an AlexaFluor^® 488 Goat anti-Rabbit secondary antibody was used at 1/1000 (2 μg/ml). DAPI nuclear counter stain. Positive staining on the molecular layer of rat cerebellar cortex (PMID: 1372043).

Immunohistochemistry (Frozen sections) analysis of Mouse E14 cerebellar cortex labeling Tenascin C with ab108930 at 1/100 dilution (4.27μg/ml). Tissue was fixed with 4% PFA and permeabilised with 0.2% TritonX-100. Antigen retrieval was performed using a heated citrate solution (10mM citrate PH 6.0 + 0.05% Tween-20). ab150077, an AlexaFluor^® 488 Goat anti-Rabbit secondary antibody was used at 1/1000 (2 μg/ml). DAPI nuclear counter stain. Positive staining on the molecular layer of mouse E14 cerebellar cortex (PMID: 1372043).

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse E14 spinal cord tissue sections labeling Tenascin C with ab108930 at 1/500 dilution (0.854 μg/ml). Heat mediated antigen retrieval was performed using Tris/EDTA buffer, PH9 (ab93684). A ready to use Goat Anti-Rabbit IgG H&L (HRP) secondary antibody was used. Hematoxylin counter stain. Positive staining on mesenchymal condensations during chondrogenesis of mouse E14 embryo (PMID: 9822997; PMID: 19586317; PMID: 24778247).

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse cerebellar cortex labeling Tenascin C with ab108930 at 1/500 dilution (0.854 μg/ml). Heat mediated antigen retrieval was performed using Tris/EDTA buffer, PH9 (ab93684). Hematoxylin was used to counter stain. A ready to use Goat Anti-Rabbit IgG H&L (HRP) secondary antibody was used. Staining on the molecular layer of mouse cerebellar cortex (PMID: 1372043).

Blocking and diluting buffer and concentration: 5% NFDM/TBST.

Exposure Time:
Lane 1: 15 seconds
Lane 2: 30 seconds

The molecular weight observed is consistent with what has been described in the literature (PMID: 10462531).

Blocking and diluting buffer and concentration: 5% NFDM /TBST.

Liver is negative control (PMID: 1717349). The molecular weight observed is consistent with what has been described in the literature (PMID: 10462531).