Recombinant
RabMAb

Recombinant Anti-Tenascin C antibody [EPR4219] (HRP) (ab196918)

Overview

  • Product name

    Anti-Tenascin C antibody [EPR4219] (HRP)
    See all Tenascin C primary antibodies
  • Description

    Rabbit monoclonal [EPR4219] to Tenascin C (HRP)
  • Host species

    Rabbit
  • Conjugation

    HRP
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) corresponding to Human Tenascin C aa 2150 to the C-terminus.

  • Positive control

    • WB: Human fetal brain tissue lysate. IHC-P: Embryonic mouse brain (e17).
  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab196918 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/5000. Detects a band of approximately 270, 230 kDa (predicted molecular weight: 241 kDa).

Target

  • Function

    Extracellular matrix protein implicated in guidance of migrating neurons as well as axons during development, synaptic plasticity as well as neuronal regeneration. Promotes neurite outgrowth from cortical neurons grown on a monolayer of astrocytes. Ligand for integrins alpha-8/beta-1, alpha-9/beta-1, alpha-V/beta-3 and alpha-V/beta-6.
  • Sequence similarities

    Belongs to the tenascin family.
    Contains 15 EGF-like domains.
    Contains 1 fibrinogen C-terminal domain.
    Contains 15 fibronectin type-III domains.
  • Cellular localization

    Secreted > extracellular space > extracellular matrix.
  • Information by UniProt
  • Database links

  • Alternative names

    • 150 225 antibody
    • Cytotactin antibody
    • Glioma associated extracellular matrix antigen antibody
    • Glioma-associated-extracellular matrix antigen antibody
    • GMEM antibody
    • GP 150 225 antibody
    • GP 150-225 antibody
    • GP antibody
    • Hexabrachion antibody
    • HXB antibody
    • JI antibody
    • MGC167029 antibody
    • Miotendinous antigen antibody
    • Myotendinous antigen antibody
    • Neuronectin antibody
    • TENA_HUMAN antibody
    • Tenascin antibody
    • Tenascin-C antibody
    • Tenascin-C isoform 14/AD1/16 antibody
    • TenascinC antibody
    • TN antibody
    • TN C antibody
    • TN-C antibody
    • TNC antibody
    see all

Images

  • Anti-Tenascin C antibody [EPR4219] (HRP) (ab196918) at 1/5000 dilution + Brain (Human) Tissue Lysate - fetal normal tissue at 10 µg

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 241 kDa
    Observed band size: 230,270 kDa
    why is the actual band size different from the predicted?


    Exposure time: 20 minutes


    This blot was produced using a 3-8% Tris Acetate gel under the TA buffer system. The gel was run at 150V for 60 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 3% milk before being incubated with ab196918 overnight at 4°C. Antibody binding was visualised using ECL development solution ab133406.

References

ab196918 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab196918.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Sign up