Recombinant Anti-Tenascin C antibody [EPR4219] - Low endotoxin, Azide free (ab215369)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR4219] to Tenascin C - Low endotoxin, Azide free
- Suitable for: WB, IHC-P, IHC-Fr
- Reacts with: Mouse, Rat, Human
Overview
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Product name
Anti-Tenascin C antibody [EPR4219] - Low endotoxin, Azide free
See all Tenascin C primary antibodies -
Description
Rabbit monoclonal [EPR4219] to Tenascin C - Low endotoxin, Azide free -
Host species
Rabbit -
Specificity
IHC on human tissues which we tested (such as testis, pancreas and stomach) showed non-specific staining. We don’t recommend this antibody for IHC on human tissues.
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Tested applications
Suitable for: WB, IHC-P, IHC-Frmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- Human testis tissue; Human fetal brain lysate.
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General notes
ab215369 is the carrier-free version of ab108930.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Our Low endotoxin, azide-free formats have low endotoxin level (≤ 1 EU/ml, determined by the LAL assay) and are free from azide, to achieve consistent experimental results in functional assays.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.20
Constituent: PBS -
Carrier free
Yes -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR4219 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Conjugation kits
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab215369 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
Use at an assay dependent concentration. Predicted molecular weight: 241 kDa.
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IHC-P |
Use at an assay dependent concentration.
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IHC-Fr |
Use at an assay dependent concentration.
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Notes |
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WB
Use at an assay dependent concentration. Predicted molecular weight: 241 kDa. |
IHC-P
Use at an assay dependent concentration. |
IHC-Fr
Use at an assay dependent concentration. |
Target
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Function
Extracellular matrix protein implicated in guidance of migrating neurons as well as axons during development, synaptic plasticity as well as neuronal regeneration. Promotes neurite outgrowth from cortical neurons grown on a monolayer of astrocytes. Ligand for integrins alpha-8/beta-1, alpha-9/beta-1, alpha-V/beta-3 and alpha-V/beta-6. -
Sequence similarities
Belongs to the tenascin family.
Contains 15 EGF-like domains.
Contains 1 fibrinogen C-terminal domain.
Contains 15 fibronectin type-III domains. -
Cellular localization
Secreted > extracellular space > extracellular matrix. - Information by UniProt
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Database links
- Entrez Gene: 3371 Human
- Entrez Gene: 21923 Mouse
- Entrez Gene: 116640 Rat
- Omim: 187380 Human
- SwissProt: P24821 Human
- SwissProt: Q80YX1 Mouse
- Unigene: 143250 Human
- Unigene: 454219 Mouse
see all -
Alternative names
- 150 225 antibody
- Cytotactin antibody
- Glioma associated extracellular matrix antigen antibody
see all
Images
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Immunohistochemistry (Frozen sections) analysis of rat cerebellar cortex labeling Tenascin C with ab108930 at 1/100 dilution (4.27μg/ml). Tissue was fixed with 4% PFA and permeabilized with 0.2% TritonX-100. Antigen retrieval was performed using a heated citrate solution (10mM citrate pH 6.0 + 0.05% Tween-20). ab150077, an Alexa Fluor® 488 Goat anti-Rabbit secondary antibody was used at 1/1000 (2 μg/ml). DAPI nuclear counterstain.
Positive staining on the molecular layer of rat cerebellar cortex (PMID: 1372043) is observed.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab108930).
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse cerebellar cortex labeling Tenascin C with ab108930 at 1/500 dilution (0.854 μg/ml). Heat mediated antigen retrieval was performed using Tris/EDTA buffer, pH 9 (ab93684). Hematoxylin was used to counterstain. A ready to use Goat Anti-Rabbit IgG H&L (HRP) secondary antibody was used. Staining on the molecular layer of mouse cerebellar cortex (PMID: 1372043) is observed.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab108930).
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Immunohistochemistry (Frozen sections) analysis of mouse E14 spinal cord labeling Tenascin C with ab108930 at 1/100 dilution (4.27μg/ml). Tissue was fixed with 4% PFA and permeabilized with 0.2% TritonX-100. Antigen retrieval was performed using a heated citrate solution (10mM citrate PH 6.0 + 0.05% Tween-20). ab150077, an Alexa Fluor® 488 Goat anti-Rabbit secondary antibody was used at 1/1000 (2 μg/ml). DAPI nuclear counterstain.
Positive staining on mesenchymal condensations during chondrogenesis of mouse E14 embryo (PMID: 9822997; PMID: 19586317; PMID: 24778247) is observed.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab108930).
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Immunohistochemistry (Frozen sections) analysis of mouse E14 cerebellar cortex labeling Tenascin C with ab108930 at 1/100 dilution (4.27μg/ml). Tissue was fixed with 4% PFA and permeabilized with 0.2% TritonX-100. Antigen retrieval was performed using a heated citrate solution (10mM citrate pH 6.0 + 0.05% Tween-20). ab150077, an Alexa Fluor® 488 Goat anti-Rabbit secondary antibody was used at 1/1000 (2 μg/ml). DAPI nuclear counterstain.
Positive staining on the molecular layer of mouse E14 cerebellar cortex (PMID: 1372043) is observed.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab108930).
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of rat cerebellar cortex labeling Tenascin C with ab108930 at 1/500 dilution (0.854 μg/ml). Heat mediated antigen retrieval was performed using Tris/EDTA Buffer, pH 9 (ab93684). A ready to use Goat Anti-Rabbit IgG H&L (HRP) secondary antibody was used. Hematoxylin counterstain. Staining on the molecular layer of rat cerebellar cortex (PMID: 1372043) is observed.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab108930).
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse E14 spinal cord tissue sections labeling Tenascin C with ab108930 at 1/500 dilution (0.854 μg/ml). Heat mediated antigen retrieval was performed using Tris/EDTA buffer, pH 9 (ab93684). A ready to use Goat Anti-Rabbit IgG H&L (HRP) secondary antibody was used. Hematoxylin counterstain.
Positive staining on mesenchymal condensations during chondrogenesis of mouse E14 embryo (PMID: 9822997; PMID: 19586317; PMID: 24778247) is observed.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab108930).
Protocols
Datasheets and documents
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Datasheet download
Certificate of Compliance
References (3)
ab215369 has been referenced in 3 publications.
- Byron A et al. Glomerular Cell Cross-Talk Influences Composition and Assembly of Extracellular Matrix. J Am Soc Nephrol N/A:N/A (2014). PubMed: 24436469
- Govindarajan P et al. Bone matrix, cellularity, and structural changes in a rat model with high-turnover osteoporosis induced by combined ovariectomy and a multiple-deficient diet. Am J Pathol 184:765-77 (2014). IHC ; Rat . PubMed: 24384131
- Iwata J et al. Noncanonical transforming growth factor ß (TGFß) signaling in cranial neural crest cells causes tongue muscle developmental defects. J Biol Chem 288:29760-70 (2013). WB, IHC ; Mouse . PubMed: 23950180