Overview

  • Product name

    Anti-Tenascin C antibody [SPM319] - BSA and Azide free
    See all Tenascin C primary antibodies
  • Description

    Mouse monoclonal [SPM319] to Tenascin C - BSA and Azide free
  • Host species

    Mouse
  • Tested applications

    Suitable for: ICC/IF, Flow Cyt, IHC-Pmore details
  • Species reactivity

    Reacts with: Human
    Does not react with: Rat
  • Immunogen

    Tissue, cells or virus corresponding to Human Tenascin C. Human breast carcinoma
    Database link: P24821

  • Positive control

    • Human lung carcinoma tissue; Extracellular matrix in tonsil and blood vessels. Stroma of many tumors such as breast, squamous cell, and lung carcinomas. Staining of normal fibroblasts serves as internal positive control

Properties

Applications

Our Abpromise guarantee covers the use of ab212463 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 0.5 - 1 µg/ml.
Flow Cyt Use 0.5-1µg for 106 cells.

in 0.1 ml

IHC-P Use a concentration of 2 - 4 µg/ml. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

for 30 minutes at room temperature.

Target

  • Function

    Extracellular matrix protein implicated in guidance of migrating neurons as well as axons during development, synaptic plasticity as well as neuronal regeneration. Promotes neurite outgrowth from cortical neurons grown on a monolayer of astrocytes. Ligand for integrins alpha-8/beta-1, alpha-9/beta-1, alpha-V/beta-3 and alpha-V/beta-6.
  • Sequence similarities

    Belongs to the tenascin family.
    Contains 15 EGF-like domains.
    Contains 1 fibrinogen C-terminal domain.
    Contains 15 fibronectin type-III domains.
  • Cellular localization

    Secreted > extracellular space > extracellular matrix.
  • Information by UniProt
  • Database links

  • Alternative names

    • 150 225 antibody
    • Cytotactin antibody
    • Glioma associated extracellular matrix antigen antibody
    • Glioma-associated-extracellular matrix antigen antibody
    • GMEM antibody
    • GP 150 225 antibody
    • GP 150-225 antibody
    • GP antibody
    • Hexabrachion antibody
    • HXB antibody
    • JI antibody
    • MGC167029 antibody
    • Miotendinous antigen antibody
    • Myotendinous antigen antibody
    • Neuronectin antibody
    • TENA_HUMAN antibody
    • Tenascin antibody
    • Tenascin-C antibody
    • Tenascin-C isoform 14/AD1/16 antibody
    • TenascinC antibody
    • TN antibody
    • TN C antibody
    • TN-C antibody
    • TNC antibody
    see all

Images

  • Immunohistochemical analysis of formalin fixed, paraffin embedded Human lung carcinoma tissue labeling Tenascin C with ab212463 at 4 μg/ml.

References

ab212463 has not yet been referenced specifically in any publications.

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