Anti-Tensin 1 antibody (ab233133)
Key features and details
- Rabbit polyclonal to Tensin 1
- Suitable for: IHC-P, WB
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
-
Product name
Anti-Tensin 1 antibody
See all Tensin 1 primary antibodies -
Description
Rabbit polyclonal to Tensin 1 -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, WBmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Cow -
Immunogen
Recombinant fragment (His-T7-tag) corresponding to Human Tensin 1 aa 1-350. Two N-terminal tags. Expressed in E.coli.
Database link: Q9HBL0 -
Positive control
- WB: Recombinant human Tensin 1 protein; Mouse heart lysate. IHC-P: Human stomach tissue.
-
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.011% Proclin 300
Constituents: 55.77% Glycerol (glycerin, glycerine), 44.219% PBS -
Concentration information loading...
-
Purity
Immunogen affinity purified -
Purification notes
Antigen-specific affinity chromatography followed by Protein A affinity chromatography. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
-
Compatible Secondaries
-
Isotype control
-
Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab233133 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
IHC-P |
Use a concentration of 5 - 20 µg/ml.
|
|
WB |
Use a concentration of 0.5 - 2 µg/ml. Predicted molecular weight: 186 kDa.
|
Notes |
---|
IHC-P
Use a concentration of 5 - 20 µg/ml. |
WB
Use a concentration of 0.5 - 2 µg/ml. Predicted molecular weight: 186 kDa. |
Target
-
Function
Involved in fibrillar adhesion formation. May be involved in cell migration, cartilage development and in linking signal transduction pathways to the cytoskeleton. -
Tissue specificity
Ubiquitous. -
Sequence similarities
Contains 1 C2 tensin-type domain.
Contains 1 phosphatase tensin-type domain.
Contains 1 SH2 domain. -
Post-translational
modificationsRapidly cleaved by calpain II. -
Cellular localization
Cell surface. Cell junction, focal adhesion. Cytoplasm, cytoskeleton. Localized at cell periphery preferentially to fibrillar adhesions than focal adhesions. Translocates from the cell edge to cell center in an ITGB1BP1-dependent manner. - Information by UniProt
-
Database links
- Entrez Gene: 286798 Cow
- Entrez Gene: 7145 Human
- Entrez Gene: 21961 Mouse
- Omim: 600076 Human
- SwissProt: Q9GLM4 Cow
- SwissProt: Q9HBL0 Human
- Unigene: 471381 Human
-
Alternative names
- matrix-remodelling associated 6 antibody
- Matrix-remodelling-associated protein 6 antibody
- MGC88584 antibody
see all
Images
-
Anti-Tensin 1 antibody (ab233133) at 2 µg/ml + Recombinant human Tensin 1 protein
Predicted band size: 186 kDa -
Anti-Tensin 1 antibody (ab233133) at 2 µg/ml + Mouse heart tissue lysate
Predicted band size: 186 kDa -
Formalin-fixed, paraffin-embedded human stomach tissue stained for Tensin 1 using ab233133 at 20 μg/ml in immunohistochemical analysis. DAB staining.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
-
SDS download
-
Datasheet download
References (1)
ab233133 has been referenced in 1 publication.
- Maniou E et al. Hindbrain neuropore tissue geometry determines asymmetric cell-mediated closure dynamics in mouse embryos. Proc Natl Acad Sci U S A 118:N/A (2021). PubMed: 33941697