Overview

  • Product name

    Anti-TEP1 antibody
  • Description

    Rabbit polyclonal to TEP1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide corresponding to Human TEP1 (N terminal). 20 amino acid from near the N-terminus of Human TEP1.

  • Positive control

    • Human skin tissue. Human breast tissue.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.02% Sodium azide
    Constituent: PBS
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab64189 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent concentration. Predicted molecular weight: 290 kDa.
IHC-P Use a concentration of 5 µg/ml.

Target

  • Relevance

    Telomerase is an RNA-dependent DNA polymerase that uses an RNA component to add telomeric repeat sequences at the ends of chromosomes. Besides the RNA component which serves as the template that specifies the telomeric repeat, the telomerase complex contains a reverse transcriptase protein (TRT) and various accessory proteins including the telomerase-associated protein 1 (TEP1). Telomerase activity is low in most somatic cells, causing the gradual shortening of telomeres which can ultimately lead to telomere fusion and cell death. High levels of telomerase activity are widely seen in cancerous cells and while recent experiments have suggested that telomerase may be a viable target in cancer therapy, expression levels of TEP1 do not correlate with malignancy. At least two isoforms of TP1 are known to exist.
  • Cellular localization

    Nuclear (probable). Telomere.
  • Database links

  • Alternative names

    • p240 antibody
    • p80 telomerase homolog antibody
    • Telomerase associated protein 1 antibody
    • Telomerase protein component 1 antibody
    • TLP1 antibody
    • TP1 antibody
    • TROVE domain family, member 1 antibody
    • TROVE1 antibody
    • VAULT2 antibody
    see all

Images

  • ab64189 (5ug/ml) staining TEP1 in human skin tissue by immunohistochemistry using formalin-fixed, paraffin-embedded tissue.
  • ab64189 (5ug/ml) staining TEP1 in human breast tissue by immunohistochemistry using formalin-fixed, paraffin embedded tissue.

References

ab64189 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Application
Western blot
Sample
Human Cell lysate - whole cell (Lane 1: SCC4 (oral cancer) lane 2: A549 (lung canc)
Gel Running Conditions
Reduced Denaturing (7.5% separating gel)
Loading amount
10 µg
Specification
Lane 1: SCC4 (oral cancer) lane 2: A549 (lung canc
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C

Stuart Hunt

Verified customer

Submitted Oct 17 2018

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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