Recombinant
RabMAb

Anti-TEPP [EPR11962(2)] antibody - N-terminal (ab191427)

Overview

  • Product name
    Anti-TEPP [EPR11962(2)] antibody - N-terminal
  • Description
    Rabbit monoclonal [EPR11962(2)] to TEPP - N-terminal
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IPmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human TEPP aa 1-100 (N terminal). The exact sequence is proprietary.
    Database link: Q6URK8

  • Positive control
    • PC-3, JAR, Human seminoma, Human testis, Human fetal liver, C6, Raw264.7, PC-12, NIH3T3, Mouse brain, Rat brain and Rat heart lysates.
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol, 0.05% BSA
  • Concentration information loading...
  • Purity
    Protein A purified
  • Clonality
    Monoclonal
  • Clone number
    EPR11962(2)
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab191427 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/20000. Detects a band of approximately 31 kDa (predicted molecular weight: 31 kDa).
IP 1/50.

Target

Images

  • All lanes : Anti-TEPP [EPR11962(2)] antibody - N-terminal (ab191427) at 1/10000 dilution

    Lane 1 : PC-3 lysate
    Lane 2 : JAR lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 31 kDa
    Additional bands at: 31 kDa. We are unsure as to the identity of these extra bands.

  • All lanes : Anti-TEPP [EPR11962(2)] antibody - N-terminal (ab191427) at 1/20000 dilution

    Lane 1 : Human seminoma lysate
    Lane 2 : Human testis lysate
    Lane 3 : Human fetal liver lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

    Predicted band size: 31 kDa
    Additional bands at: 31 kDa. We are unsure as to the identity of these extra bands.

  • All lanes : Anti-TEPP [EPR11962(2)] antibody - N-terminal (ab191427) at 1/1000 dilution

    Lane 1 : C6 lysate
    Lane 2 : Raw264.7 lysate
    Lane 3 : PC-12 lysate
    Lane 4 : NIH3T3 lysate
    Lane 5 : Mouse brain lysate
    Lane 6 : Rat brain lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG , (H+L),Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 31 kDa
    Additional bands at: 31 kDa. We are unsure as to the identity of these extra bands.

  • Immunoprecipitation of TEPP from PC-3 lysate using ab191427 at 1/50 or 1X PBS (negative control) (lane 2). Immunostained with ab191427 at 1/1000 and HRP-conjugated anti-rabbit IgG preferentially detecting the non-reduced form of rabbit IgG.

References

ab191427 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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