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Thank you for your response, I see you have a very good HPLC trace!
I use the same buffer for my HPLC (MeCN +0.1% TFA but from 20-40%)so I
wouldn't expect to see such different results. What temperature do you run
it at? Could this have an effect perhaps? I see you prepare your tertiapin
Q in water, I use pH6.2 phosphate buffer (50nmM)for our experiments - I
wouldn't expect this to matter so much as it soon drops in pH when in the
Asked on Jan 17 2012