Key features and details
- Rabbit polyclonal to Testin
- Suitable for: IHC-P, WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
Product nameAnti-Testin antibody
DescriptionRabbit polyclonal to Testin
Tested applicationsSuitable for: IHC-P, WB, ICC/IFmore details
Species reactivityReacts with: Human
Predicted to work with: Sheep, Cow, Cat, Dog, Pig, Chimpanzee, Ferret, Indian muntjac, Baboon, African green monkey, Common marmoset, Orangutan, Nomascus, Squirrel monkey, Aotus, Black lemur, Clouded leopard, Spider monkey, Colobus
- IHC-P: Human tonsil tissue. WB: EFO-21 cell lysate. ICC/IF: SiHa cells.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), PBS
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab224375 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|WB||Use a concentration of 0.04 - 0.4 µg/ml. Predicted molecular weight: 48 kDa.|
|ICC/IF||Use a concentration of 0.25 - 2 µg/ml.
Fixation/Permeabilization: PFA/Triton X-100.
FunctionScaffold protein that may play a role in cell adhesion, cell spreading and in the reorganization of the actin cytoskeleton. Plays a role in the regulation of cell proliferation. May act as a tumor suppressor. Inhibits tumor cell growth.
Sequence similaritiesBelongs to the prickle / espinas / testin family.
Contains 3 LIM zinc-binding domains.
Contains 1 PET domain.
DomainThe N-terminal and the C-terminal halves of the protein can associate with each other, thereby hindering interactions with ZYX.
Cellular localizationCytoplasm. Cell junction > focal adhesion. Detected along actin stress fibers.
- Information by UniProt
- DKFZP586B2022 antibody
- MGC1146 antibody
- TES antibody
Anti-Testin antibody (ab224375) at 1/100 dilution + EFO-21 cell lysate
Developed using the ECL technique.
Predicted band size: 48 kDa
Paraffin-embedded human tonsil tissue stained for Testin using ab224375 at 1/50 dilution in immunohistochemical analysis.
PFA-fixed, Triton X-100 permeabilized SiHa cells stained for Testin (green) using ab224375 at 4 µg/ml in ICC/IF.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab224375 has not yet been referenced specifically in any publications.