Overview

  • Product name
    Testosterone ELISA Kit
    See all Testosterone kits
  • Detection method
    Colorimetric
  • Precision
    Intra-assay
    Sample n Mean SD CV%
    Control Sera 48 <=5.8%
    Inter-assay
    Sample n Mean SD CV%
    Control Sera 48 <=10.5%
  • Sample type
    Urine, Serum, Heparin Plasma, Citrate Plasma
  • Assay type
    Competitive
  • Sensitivity
    = 0.07 ng/ml
  • Range
    0.2 ng/ml - 16 ng/ml
  • Recovery

    = 105.5 %

  • Assay duration
    Multiple steps standard assay
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Product overview

    Abcam’s Testosterone in vitro competitive ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate quantitative measurement of Testosterone in serum and plasma.

    A 96-well plate has been precoated with anti-Testosterone antibodies. Samples and the Testosterone-HRP conjugate are added to the wells, where Testosterone in the sample competes with the added Testosterone-HRP for antibody binding. After incubation, the wells are washed to remove unbound material and TMB substrate is then added which is catalyzed by HRP to produce blue coloration. The reaction is terminated by addition of Stop Solution which stops the color development and produces a color change from blue to yellow. The intensity of signal is inversely proportional to the amount of Testosterone in the sample and the intensity is measured at 450 nm.

  • Tested applications
    Suitable for: Competitive ELISAmore details
  • Platform
    Microplate

Properties

Applications

Our Abpromise guarantee covers the use of ab108666 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Competitive ELISA Use at an assay dependent dilution.

Images

  • Testosterone measured in biological fluids showing quantity (ng) per mL of tested sample. Samples were diluted 1-2 fold.

  • Representative Standard Curve using ab108666

Protocols

References

This product has been referenced in:
  • Faisal I  et al. Transcription Factor USF1 Is Required for Maintenance of Germline Stem Cells in Male Mice. Endocrinology 160:1119-1136 (2019). Read more (PubMed: 30759202) »
  • Salahshoor MR  et al. Effect of Thymoquinone on Reproductive Parameter in Morphine-treated Male Mice. Adv Biomed Res 7:18 (2018). Read more (PubMed: 29456989) »
See all 11 Publications for this product

Customer reviews and Q&As

1-10 of 13 Abreviews or Q&A

Question
Answer

Testosterone is a biochemical and it should be same in all the species so theoretically this kit would work with testosterone from all the species. We however have tested it with human samples only. Please note the amount of testosterone is higher in urine as compared to serum and plasma samples.

Read More

Easy to use but maybe not suitable for mouse serum

Below average Excellent 5/5 (Ease of Use)
Abreviews
We tried to use this kit to measure testosterone in mouse serum but were unable to measure anything in serum from normal WT mice. I suspect matrix effect interfered with measurements in neat or 1:2 diluted samples and more dilute samples (1 to 10) might have had levels below the detection limit. It is possible that further optimization or testing of other dilutions may help.
The standard worked really well and the product was easy to use. I would consider this kit if I ever have to measure testosterone in human samples but not for mouse.

Abcam user community

Verified customer

Submitted Oct 23 2018

Works in guinea pig plasma

Excellent Excellent 5/5 (Ease of Use)
Abreviews
Bought this kit to try with guinea pig plasma. Kit was easy to use, fast and instructions were clear. From my results, it seems to have worked well in the guinea pig. Male samples all fell within the curve at expected concentrations.

Abcam user community

Verified customer

Submitted May 26 2017

Question
Answer

Samples can be diluted in PBS.

Read More

Answer

This assay will measure total testosterone, free and also protein-bound. To measure just free testosterone, we have another assay, https://www.abcam.com/human-testosterone-free-elisa-kit-ab178663.html.


We recommend running the two controls in duplicate, so those will require 4 wells. The five standards in duplicate will require 10 wells. One more well is needed for the substrate blank.

Read More

Question
Answer

I can confirm the lab used PBS to dilute serum samples when validating the kit.

Read More

Answer

This is most likely due to matrix effect which affect measurement of analyte of interest in ELISA when undiluted serum or plasma samples are used. Matrix effect can arise from a number of matrix components such as phospholipids, carbohydrates, and endogenous metabolites (bilirubin), residual formulation components from intraperitoneal (ip), intravenous (iv), or oral dosing (po) experiments; for example, polyethylene glycol (PEG), solutol, polysorbate (Tween 80), etc, an interaction between the analyte of interest and the matrix, such as covalent binding to plasma proteins or the enzymatic degradation of a prodrug.  The easiest way to overcome this is by using diluted samples e.g. 1/2 or 1/10 dilution.

Read More

Answer

The test measures both bound and free Testosterone.

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Testosterone Human Elisa Kit

Excellent Below average 2/5 (Ease of Use)
Abreviews
This Kit is very easy to use. It provides all the reagent and it is very good for data output as it takes less than 3 hours to be performed.
The kit is designed for Human Serum of plasma. However, it works very well on mouse serum.
Standards provided with the kit, produce a reliable standard curve as shown in the picture below.

Dr. Francesco Elia Marino

Verified customer

Submitted Aug 21 2014

Answer


I confirmed with the lab and unfortunately do not have good news:
The heavily hemolyzed samples would have a great influence on the results. We do not know of any protocol trick to circumvent this and therefore, recommend to collect new samples.
But we would assume that all samples would interfere with the assay at the same level.

Read More

1-10 of 13 Abreviews or Q&A

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