Testosterone ELISA Kit (ab108666)
Key features and details
- Sensitivity: 0.07 ng/ml
- Range: 0.2 ng/ml - 16 ng/ml
- Sample type: Cit plasma, Hep Plasma, Serum, Urine
- Detection method: Colorimetric
- Assay type: Competitive
- Reacts with: Mouse, Rat, Human
Overview
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Product name
Testosterone ELISA Kit
See all Testosterone kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Control Sera 48 <=5.8% Inter-assay Sample n Mean SD CV% Control Sera 48 <=10.5% -
Sample type
Urine, Serum, Hep Plasma, Cit plasma -
Assay type
Competitive -
Sensitivity
= 0.07 ng/ml -
Range
0.2 ng/ml - 16 ng/ml -
Recovery
= 105.5 %
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Assay duration
Multiple steps standard assay -
Species reactivity
Reacts with: Mouse, Rat, Human -
Product overview
Testosterone ELISA kit is a competitive ELISA designed for the accurate quantitative measurement of Testosterone in serum and plasma.
A 96-well plate has been precoated with anti-Testosterone antibodies. Samples and the Testosterone-HRP conjugate are added to the wells, where Testosterone in the sample competes with the added Testosterone-HRP for antibody binding. After incubation, the wells are washed to remove unbound material and TMB substrate is then added which is catalyzed by HRP to produce blue coloration. The reaction is terminated by addition of Stop Solution which stops the color development and produces a color change from blue to yellow. The intensity of signal is inversely proportional to the amount of Testosterone in the sample and the intensity is measured at 450 nm.
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Platform
Microplate
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 1 x 96 tests 10X Wash Buffer 1 x 50ml Cover foils 1 unit Stop Solution 1 x 15ml Strip holder 1 unit Testosterone conjugate 1 x 12ml Testosterone Control A 1 x 1ml Testosterone Control B 1 x 1ml Testosterone IgG Coated Microtiter Plate (12 x 8 wells) 1 unit Testosterone Standard 0 - 0.0 ng/mL 1 x 1ml Testosterone Standard 1 - 0.2 ng/mL 1 x 1ml Testosterone Standard 2 - 1.0 ng/mL 1 x 1ml Testosterone Standard 3 - 4.0 ng/mL 1 x 1ml Testosterone Standard 4 - 16.0 ng/mL 1 x 1ml TMB Substrate Solution 1 x 15ml -
Research areas
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Alternative names
- 17ß-hydroxyandrost-4-ene-3-one
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Database links
- SwissProt: P04278 Human
Images
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Biological fluids for ab108666
Testosterone measured in biological fluids showing quantity (ng) per mL of tested sample. Samples were diluted 1-2 fold.
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Typical Standard CurveRepresentative Standard Curve using ab108666
Datasheets and documents
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SDS download
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Datasheet download
References (35)
ab108666 has been referenced in 35 publications.
- Chen J et al. Decreased blood vessel density and endothelial cell subset dynamics during ageing of the endocrine system. EMBO J 40:e105242 (2021). PubMed: 33215738
- Venditti M et al. Preliminary Investigation on the Ameliorative Role Exerted by D-Aspartic Acid in Counteracting Ethane Dimethane Sulfonate (EDS) Toxicity in the Rat Testis. Animals (Basel) 11:N/A (2021). PubMed: 33435542
- Khaliq W et al. Lipid metabolic signatures deviate in sepsis survivors compared to non-survivors. Comput Struct Biotechnol J 18:3678-3691 (2020). PubMed: 33304464
- Morgan HL et al. Low protein diet and methyl-donor supplements modify testicular physiology in mice. Reproduction 159:627-641 (2020). PubMed: 32163913
- Stratton MT et al. Four Weeks of Time-Restricted Feeding Combined with Resistance Training Does Not Differentially Influence Measures of Body Composition, Muscle Performance, Resting Energy Expenditure, and Blood Biomarkers. Nutrients 12:N/A (2020). PubMed: 32316561