• Product name
    Testosterone ELISA Kit
    See all Testosterone kits
  • Detection method
  • Precision
    Sample n Mean SD CV%
    Control Sera 48 <=5.8%
    Sample n Mean SD CV%
    Control Sera 48 <=10.5%
  • Sample type
    Urine, Serum, Heparin Plasma, Citrate Plasma
  • Assay type
  • Sensitivity
    = 0.07 ng/ml
  • Range
    0.2 ng/ml - 16 ng/ml
  • Recovery

    = 105.5 %

  • Assay duration
    Multiple steps standard assay
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Product overview

    Abcam’s Testosterone in vitro competitive ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate quantitative measurement of Testosterone in serum and plasma.

    A 96-well plate has been precoated with anti-Testosterone antibodies. Samples and the Testosterone-HRP conjugate are added to the wells, where Testosterone in the sample competes with the added Testosterone-HRP for antibody binding. After incubation, the wells are washed to remove unbound material and TMB substrate is then added which is catalyzed by HRP to produce blue coloration. The reaction is terminated by addition of Stop Solution which stops the color development and produces a color change from blue to yellow. The intensity of signal is inversely proportional to the amount of Testosterone in the sample and the intensity is measured at 450 nm.

  • Tested applications
    Suitable for: Competitive ELISAmore details
  • Platform



Our Abpromise guarantee covers the use of ab108666 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Competitive ELISA Use at an assay dependent dilution.


  • Testosterone measured in biological fluids showing quantity (ng) per mL of tested sample. Samples were diluted 1-2 fold.

  • Representative Standard Curve using ab108666



This product has been referenced in:
  • Salahshoor MR  et al. Effect of Thymoquinone on Reproductive Parameter in Morphine-treated Male Mice. Adv Biomed Res 7:18 (2018). Read more (PubMed: 29456989) »
  • Sakr HF  et al. Possible mechanisms underlying fatty liver in a rat model of male hypogonadism: A protective role for testosterone. Steroids 135:21-30 (2018). Read more (PubMed: 29674209) »

See all 8 Publications for this product

Customer reviews and Q&As

Testosterone is a biochemical and it should be same in all the species so theoretically this kit would work with testosterone from all the species. We however have tested it with human samples only. Please note the amount of testosterone is higher in ...

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Works in guinea pig plasma

Excellent Excellent 5/5 (Ease of Use)
Bought this kit to try with guinea pig plasma. Kit was easy to use, fast and instructions were clear. From my results, it seems to have worked well in the guinea pig. Male samples all fell within the curve at expected concentrations.

Abcam user community

Verified customer

Submitted May 26 2017

Samples can be diluted in PBS.

This assay will measure total testosterone, free and also protein-bound. To measure just free testosterone, we have another assay, https://www.abcam.com/human-testosterone-free-elisa-kit-ab178663.html.

We recommend running the two control...

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I can confirm the lab used PBS to dilute serum samples when validating the kit.

This is most likely due to matrix effect which affect measurement of analyte of interest in ELISA when undiluted serum or plasma samples are used. Matrix effect can arise from a number of matrix components such as phospholipids, carbohydrates, and endo...

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The test measures both bound and free Testosterone.

Testosterone Human Elisa Kit

Excellent Below average 2/5 (Ease of Use)
This Kit is very easy to use. It provides all the reagent and it is very good for data output as it takes less than 3 hours to be performed.
The kit is designed for Human Serum of plasma. However, it works very well on mouse serum.
Standards provided with the kit, produce a reliable standard curve as shown in the picture below.

Dr. Francesco Elia Marino

Verified customer

Submitted Aug 21 2014

I confirmed with the lab and unfortunately do not have good news:
The heavily hemolyzed samples would have a great influence on the results. We do not know of any protocol trick to circumvent this and therefore, recommend to collect new sampl...

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Thank you for contacting us regarding these products.

In regards to the two testosterone kits, ab108666 and 108658, they both measure total testosterone. Please see a comparison/differences of the two kits below. I am confident that you wou...

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