Testosterone ELISA Kit (ab108666)

Reviews (3)Q&A (10)References (19)

Overview

  • Product name

    Testosterone ELISA Kit
    See all Testosterone kits

  • Detection method

    Colorimetric

  • Precision

    Intra-assay
    Sample n Mean SD CV%
    Control Sera 48 <=5.8%
    Inter-assay
    Sample n Mean SD CV%
    Control Sera 48 <=10.5%

  • Sample type

    Urine, Serum, Hep Plasma, Cit plasma

  • Assay type

    Competitive

  • Sensitivity

    = 0.07 ng/ml

  • Range

    0.2 ng/ml - 16 ng/ml

  • Recovery

    = 105.5 %

  • Assay duration

    Multiple steps standard assay

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Product overview

    Testosterone ELISA kit is a competitive ELISA designed for the accurate quantitative measurement of Testosterone in serum and plasma.


    A 96-well plate has been precoated with anti-Testosterone antibodies. Samples and the Testosterone-HRP conjugate are added to the wells, where Testosterone in the sample competes with the added Testosterone-HRP for antibody binding. After incubation, the wells are washed to remove unbound material and TMB substrate is then added which is catalyzed by HRP to produce blue coloration. The reaction is terminated by addition of Stop Solution which stops the color development and produces a color change from blue to yellow. The intensity of signal is inversely proportional to the amount of Testosterone in the sample and the intensity is measured at 450 nm.

  • Platform

    Microplate

Properties

Images

  • Biological fluids for ab108666
    Biological fluids for ab108666

    Testosterone measured in biological fluids showing quantity (ng) per mL of tested sample. Samples were diluted 1-2 fold.

  • Typical Standard Curve
    Typical Standard Curve

    Representative Standard Curve using ab108666

Protocols

References

This product has been referenced in:

  • Alqahtani SA & Alhawiti NM Administration of testosterone improves the prothrombotic and antifibrinolytic parameters associated with its deficiency in an orchidectiomized rat model. Platelets 30:624-630 (2019). Read more (PubMed: 30102104) »
  • Lu YC  et al. Glycoprotein from Mytilus edulis extract inhibits lipid accumulation and improves male reproductive dysfunction in high-fat diet-induced obese rats. Biomed Pharmacother 109:369-376 (2019). Read more (PubMed: 30399571) »
See all 19 Publications for this product

Publishing research using ab108666? Please let us know so that we can cite the reference in this datasheet.

Customer reviews and Q&As

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1-10 of 13 Abreviews or Q&A

Question

Does this kit ab108666 cross react with mouse testosterone as well?

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Answer

Testosterone is a biochemical and it should be same in all the species so theoretically this kit would work with testosterone from all the species. We however have tested it with human samples only. Please note the amount of testosterone is higher in urine as compared to serum and plasma samples.

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Easy to use but maybe not suitable for mouse serum

 Below average Excellent 5/5 (Ease of Use)
Abreviews
We tried to use this kit to measure testosterone in mouse serum but were unable to measure anything in serum from normal WT mice. I suspect matrix effect interfered with measurements in neat or 1:2 diluted samples and more dilute samples (1 to 10) might have had levels below the detection limit. It is possible that further optimization or testing of other dilutions may help.
The standard worked really well and the product was easy to use. I would consider this kit if I ever have to measure testosterone in human samples but not for mouse.

Abcam user community

Verified customer

Submitted Oct 23 2018

Works in guinea pig plasma

 Excellent Excellent 5/5 (Ease of Use)
Abreviews
abreview image
Bought this kit to try with guinea pig plasma. Kit was easy to use, fast and instructions were clear. From my results, it seems to have worked well in the guinea pig. Male samples all fell within the curve at expected concentrations.

Abcam user community

Verified customer

Submitted May 26 2017

Question

I would like to know what solution to use to dilute samples since in the kit there is none.

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Answer

Samples can be diluted in PBS.

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Question

I have a couple of questions regarding this kit. First, is there a way to ascertain free testosterone from this kit as well, or is it necessary to perform a separate ELISA? Second, how many wells on the plate are used for controls?

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Answer

This assay will measure total testosterone, free and also protein-bound. To measure just free testosterone, we have another assay, https://www.abcam.com/human-testosterone-free-elisa-kit-ab178663.html.


We recommend running the two controls in duplicate, so those will require 4 wells. The five standards in duplicate will require 10 wells. One more well is needed for the substrate blank.

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Question

Which buffer can be used for diluting serum samples?

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Answer

I can confirm the lab used PBS to dilute serum samples when validating the kit.

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Question

I am using undiluted serum and plasma samples and observing high OD values. Do you have any suggestion why is this so?

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Answer

This is most likely due to matrix effect which affect measurement of analyte of interest in ELISA when undiluted serum or plasma samples are used. Matrix effect can arise from a number of matrix components such as phospholipids, carbohydrates, and endogenous metabolites (bilirubin), residual formulation components from intraperitoneal (ip), intravenous (iv), or oral dosing (po) experiments; for example, polyethylene glycol (PEG), solutol, polysorbate (Tween 80), etc, an interaction between the analyte of interest and the matrix, such as covalent binding to plasma proteins or the enzymatic degradation of a prodrug.  The easiest way to overcome this is by using diluted samples e.g. 1/2 or 1/10 dilution.

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Question

Does the assay detect both protein bound and free testosterone? Or is all testosterone considered bound to protein?

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Answer

The test measures both bound and free Testosterone.

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Testosterone Human Elisa Kit

 Excellent Below average 2/5 (Ease of Use)
Abreviews
abreview image
This Kit is very easy to use. It provides all the reagent and it is very good for data output as it takes less than 3 hours to be performed.
The kit is designed for Human Serum of plasma. However, it works very well on mouse serum.
Standards provided with the kit, produce a reliable standard curve as shown in the picture below.

Dr. Francesco Elia Marino

Verified customer

Submitted Aug 21 2014

Question

We have bought two abcam ELISA kits to dose testosterone (ab108666) and cortisol (ab108665) from human blood samples. Unfortunately, blood samples were frozen without separating blood products (no centrifugation before freezing). As a result, there has been heavy hemolysis in our samples.
Since these samples cannot be taken again, I have two questions: is it still possible to get at least a &apos;relative&apos; measure of cortisol and/or testosterone or is it absolutely impossible? is there some specific recommendations for our situation? Diluting samples? Centrifuging more to remove big cell components?

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Answer


I confirmed with the lab and unfortunately do not have good news:
The heavily hemolyzed samples would have a great influence on the results. We do not know of any protocol trick to circumvent this and therefore, recommend to collect new samples.
But we would assume that all samples would interfere with the assay at the same level.

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1-10 of 13 Abreviews or Q&A

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