Overview

  • Product name
    Testosterone ELISA Kit
    See all Testosterone kits
  • Detection method
    Colorimetric
  • Precision
    Intra-assay
    Sample n Mean SD CV%
    Control Sera 48 <=5.8%
    Inter-assay
    Sample n Mean SD CV%
    Control Sera 48 <=10.5%
  • Sample type
    Urine, Serum, Heparin Plasma, Citrate Plasma
  • Assay type
    Competitive
  • Sensitivity
    = 0.07 ng/ml
  • Range
    0.2 ng/ml - 16 ng/ml
  • Recovery

    = 105.5 %

  • Assay duration
    Multiple steps standard assay
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Product overview

    Abcam’s Testosterone in vitro competitive ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate quantitative measurement of Testosterone in serum and plasma.

    A 96-well plate has been precoated with anti-Testosterone antibodies. Samples and the Testosterone-HRP conjugate are added to the wells, where Testosterone in the sample competes with the added Testosterone-HRP for antibody binding. After incubation, the wells are washed to remove unbound material and TMB substrate is then added which is catalyzed by HRP to produce blue coloration. The reaction is terminated by addition of Stop Solution which stops the color development and produces a color change from blue to yellow. The intensity of signal is inversely proportional to the amount of Testosterone in the sample and the intensity is measured at 450 nm.

  • Tested applications
    Suitable for: Competitive ELISAmore details
  • Platform
    Microplate

Properties

Applications

Our Abpromise guarantee covers the use of ab108666 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Competitive ELISA Use at an assay dependent dilution.

Images

  • Biological fluids for ab108666
    Biological fluids for ab108666

    Testosterone measured in biological fluids showing quantity (ng) per mL of tested sample. Samples were diluted 1-2 fold.

  • Typical Standard Curve
    Typical Standard Curve

    Representative Standard Curve using ab108666

Protocols

References

This product has been referenced in:
  • Salahshoor MR  et al. Effect of Thymoquinone on Reproductive Parameter in Morphine-treated Male Mice. Adv Biomed Res 7:18 (2018). Read more (PubMed: 29456989) »
  • Sakr HF  et al. Possible mechanisms underlying fatty liver in a rat model of male hypogonadism: A protective role for testosterone. Steroids 135:21-30 (2018). Read more (PubMed: 29674209) »

See all 8 Publications for this product

Publishing research using ab108666? Please let us know so that we can cite the reference in this datasheet.

Customer reviews and Q&As

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12 reviews or Q&A

10
Votes: Highest First

Does this kit ab108666 cross react with mouse testosterone as well?

Testosterone is a biochemical and it should be same in all the species so theoretically this kit would work with testosterone from all the species. We however have tested it with human samples only. Please note the amount of testosterone is higher in ...

Read More

Works in guinea pig plasma

 Excellent Excellent 5/5 (Ease of Use)
Abreviews
Bought this kit to try with guinea pig plasma. Kit was easy to use, fast and instructions were clear. From my results, it seems to have worked well in the guinea pig. Male samples all fell within the curve at expected concentrations.
abreview image
Username

Abcam user community

Verified customer

Submitted May 26 2017

I would like to know what solution to use to dilute samples since in the kit there is none.

Samples can be diluted in PBS.

I have a couple of questions regarding this kit. First, is there a way to ascertain free testosterone from this kit as well, or is it necessary to perform a separate ELISA? Second, how many wells on the plate are used for controls?

This assay will measure total testosterone, free and also protein-bound. To measure just free testosterone, we have another assay, https://www.abcam.com/human-testosterone-free-elisa-kit-ab178663.html.


We recommend running the two control...

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Which buffer can be used for diluting serum samples?

I can confirm the lab used PBS to dilute serum samples when validating the kit.

I am using undiluted serum and plasma samples and observing high OD values. Do you have any suggestion why is this so?

This is most likely due to matrix effect which affect measurement of analyte of interest in ELISA when undiluted serum or plasma samples are used. Matrix effect can arise from a number of matrix components such as phospholipids, carbohydrates, and endo...

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Does the assay detect both protein bound and free testosterone? Or is all testosterone considered bound to protein?

The test measures both bound and free Testosterone.

Testosterone Human Elisa Kit

 Excellent Below average 2/5 (Ease of Use)
Abreviews
This Kit is very easy to use. It provides all the reagent and it is very good for data output as it takes less than 3 hours to be performed.
The kit is designed for Human Serum of plasma. However, it works very well on mouse serum.
Standards provided with the kit, produce a reliable standard curve as shown in the picture below.
abreview image
Username

Dr. Francesco Elia Marino

Verified customer

Submitted Aug 21 2014

We have bought two abcam ELISA kits to dose testosterone (ab108666) and cortisol (ab108665) from human blood samples. Unfortunately, blood samples were frozen without separating blood products (no centrifugation before freezing). As a result, there has...

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I confirmed with the lab and unfortunately do not have good news:
The heavily hemolyzed samples would have a great influence on the results. We do not know of any protocol trick to circumvent this and therefore, recommend to collect new sampl...

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Customer kindly contacted us regarding these products. They wished to know if the products measured total testosterone, whether the kits measured bound or unbound and enquired about which variant of testosterone would be the best to test for when searc...

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Thank you for contacting us regarding these products.

In regards to the two testosterone kits, ab108666 and 108658, they both measure total testosterone. Please see a comparison/differences of the two kits below. I am confident that you wou...

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1-10 of 12 Abreviews or Q&A

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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