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    testosterone-elisa-kit-ab108666.pdf

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Signal Transduction Signaling Pathway Nuclear Signaling Nuclear Hormone Receptors Testosterone
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Testosterone ELISA Kit (ab108666)

  • Datasheet
  • SDS
  • Protocol Booklet
Reviews (4)Q&A (10)References (44)

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Biological fluids for ab108666
  • Typical Standard Curve

Key features and details

  • Sensitivity: 0.07 ng/ml
  • Range: 0.2 ng/ml - 16 ng/ml
  • Sample type: Cit plasma, Hep Plasma, Serum, Urine
  • Detection method: Colorimetric
  • Assay type: Competitive
  • Reacts with: Human

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Overview

  • Product name

    Testosterone ELISA Kit
    See all Testosterone kits
  • Detection method

    Colorimetric
  • Precision

    Intra-assay
    Sample n Mean SD CV%
    Control Sera 48 <=5.8%
    Inter-assay
    Sample n Mean SD CV%
    Control Sera 48 <=10.5%
  • Sample type

    Urine, Serum, Hep Plasma, Cit plasma
  • Assay type

    Competitive
  • Sensitivity

    = 0.07 ng/ml
  • Range

    0.2 ng/ml - 16 ng/ml
  • Recovery

    = 105.5 %

  • Assay duration

    Multiple steps standard assay
  • Species reactivity

    Reacts with: Human
  • Product overview

    Testosterone ELISA kit is a competitive ELISA designed for the accurate quantitative measurement of Testosterone in serum and plasma.


    A 96-well plate has been precoated with anti-Testosterone antibodies. Samples and the Testosterone-HRP conjugate are added to the wells, where Testosterone in the sample competes with the added Testosterone-HRP for antibody binding. After incubation, the wells are washed to remove unbound material and TMB substrate is then added which is catalyzed by HRP to produce blue coloration. The reaction is terminated by addition of Stop Solution which stops the color development and produces a color change from blue to yellow. The intensity of signal is inversely proportional to the amount of Testosterone in the sample and the intensity is measured at 450 nm.

  • Platform

    Microplate

Properties

  • Storage instructions

    Store at +4°C. Please refer to protocols.
  • Components 1 x 96 tests
    10X Wash Buffer 1 x 50ml
    Cover foils 1 unit
    Stop Solution 1 x 15ml
    Strip holder 1 unit
    Testosterone conjugate 1 x 12ml
    Testosterone Control A 1 x 1ml
    Testosterone Control B 1 x 1ml
    Testosterone IgG Coated Microtiter Plate (12 x 8 wells) 1 unit
    Testosterone Standard 0 - 0.0 ng/mL 1 x 1ml
    Testosterone Standard 1 - 0.2 ng/mL 1 x 1ml
    Testosterone Standard 2 - 1.0 ng/mL 1 x 1ml
    Testosterone Standard 3 - 4.0 ng/mL 1 x 1ml
    Testosterone Standard 4 - 16.0 ng/mL 1 x 1ml
    TMB Substrate Solution 1 x 15ml
  • Research areas

    • Signal Transduction
    • Signaling Pathway
    • Nuclear Signaling
    • Nuclear Hormone Receptors
    • Testosterone
    • Signal Transduction
    • Growth Factors/Hormones
    • Hormones
    • Epigenetics and Nuclear Signaling
    • Nuclear Signaling Pathways
    • Nuclear Receptors
    • Testosterone
    • Developmental Biology
    • Reproduction
    • Hormones
    • Kits/ Lysates/ Other
    • Kits
    • ELISA Kits
    • ELISA Kits
    • Growth factors and hormones ELISA kits
  • Alternative names

    • 17ß-hydroxyandrost-4-ene-3-one
  • Database links

    • SwissProt: P04278 Human

    Images

    • Biological fluids for ab108666
      Biological fluids for ab108666

      Testosterone measured in biological fluids showing quantity (ng) per mL of tested sample. Samples were diluted 1-2 fold.

    • Typical Standard Curve
      Typical Standard Curve

      Representative Standard Curve using ab108666

    Protocols

    • Protocol Booklet

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    References (44)

    Publishing research using ab108666? Please let us know so that we can cite the reference in this datasheet.

    ab108666 has been referenced in 44 publications.

    • Chen J  et al. Decreased blood vessel density and endothelial cell subset dynamics during ageing of the endocrine system. EMBO J 40:e105242 (2021). PubMed: 33215738
    • Venditti M  et al. Preliminary Investigation on the Ameliorative Role Exerted by D-Aspartic Acid in Counteracting Ethane Dimethane Sulfonate (EDS) Toxicity in the Rat Testis. Animals (Basel) 11:N/A (2021). PubMed: 33435542
    • Van Simaeys G  et al. [18F]-JK-PSMA-7 and [18F]-FDG tumour PET uptake in treated xenograft human prostate cancer model in mice. Eur J Nucl Med Mol Imaging 48:1773-1784 (2021). PubMed: 33398412
    • Mohammed S  et al. Effect of 150?kHz electromagnetic radiation on the development of polycystic ovaries induced by estradiol Valerate in Sprague Dawley rats. J Ovarian Res 14:26 (2021). PubMed: 33546719
    • Soliman MM  et al. Impacts of n-acetyl cysteine on gibberellic acid-induced testicular dysfunction through regulation of inflammatory cytokines, steroid and antioxidant activity. Andrologia 53:e14036 (2021). PubMed: 33724537
    View all Publications for this product

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    1-10 of 14 Abreviews or Q&A

    Dr Frances Orton

    Excellent Excellent 5/5 (Ease of Use)
    Abreviews
    Abreviews
    abreview image
    I used this product for common frog (Rana temporaria) urine. I first checked the levels of testosterone in the urine by measuring dilutions of 2 test samples against the highest and lowest concentrations of the standards. From this I was able to determine that the urine should be applied to the ELISA neat and did not require any dilution.
    Levels of testosterone in the 88 samples that I tested were low, and in some cases below the lowest testosterone standard (see photograph).

    Frances Orton

    Verified customer

    Submitted Sep 04 2020

    Easy to use but maybe not suitable for mouse serum

    Below average Excellent 5/5 (Ease of Use)
    Abreviews
    Abreviews
    We tried to use this kit to measure testosterone in mouse serum but were unable to measure anything in serum from normal WT mice. I suspect matrix effect interfered with measurements in neat or 1:2 diluted samples and more dilute samples (1 to 10) might have had levels below the detection limit. It is possible that further optimization or testing of other dilutions may help.
    The standard worked really well and the product was easy to use. I would consider this kit if I ever have to measure testosterone in human samples but not for mouse.

    Abcam user community

    Verified customer

    Submitted Oct 23 2018

    Works in guinea pig plasma

    Excellent Excellent 5/5 (Ease of Use)
    Abreviews
    Abreviews
    abreview image
    Bought this kit to try with guinea pig plasma. Kit was easy to use, fast and instructions were clear. From my results, it seems to have worked well in the guinea pig. Male samples all fell within the curve at expected concentrations.

    Abcam user community

    Verified customer

    Submitted May 26 2017

    Testosterone Human Elisa Kit

    Excellent Below average 2/5 (Ease of Use)
    Abreviews
    Abreviews
    abreview image
    This Kit is very easy to use. It provides all the reagent and it is very good for data output as it takes less than 3 hours to be performed.
    The kit is designed for Human Serum of plasma. However, it works very well on mouse serum.
    Standards provided with the kit, produce a reliable standard curve as shown in the picture below.

    DR. Francesco Elia Marino

    Verified customer

    Submitted Aug 21 2014

    Question

    Does this kit ab108666 cross react with mouse testosterone as well?

    Read More

    Abcam community

    Verified customer

    Asked on Mar 19 2014

    Answer

    Testosterone is a biochemical and it should be same in all the species so theoretically this kit would work with testosterone from all the species. We however have tested it with human samples only. Please note the amount of testosterone is higher in urine as compared to serum and plasma samples.

    Read More

    Padamjeet Singh

    Abcam Scientific Support

    Answered on Mar 19 2014

    Question

    I would like to know what solution to use to dilute samples since in the kit there is none.

    Read More

    Abcam community

    Verified customer

    Asked on Dec 08 2016

    Answer

    Samples can be diluted in PBS.

    Read More

    Abcam Scientific Support

    Answered on Dec 08 2016

    Question

    I have a couple of questions regarding this kit. First, is there a way to ascertain free testosterone from this kit as well, or is it necessary to perform a separate ELISA? Second, how many wells on the plate are used for controls?

    Read More

    Abcam community

    Verified customer

    Asked on Oct 26 2016

    Answer

    This assay will measure total testosterone, free and also protein-bound. To measure just free testosterone, we have another assay, https://www.abcam.com/human-testosterone-free-elisa-kit-ab178663.html.


    We recommend running the two controls in duplicate, so those will require 4 wells. The five standards in duplicate will require 10 wells. One more well is needed for the substrate blank.

    Read More

    Abcam Scientific Support

    Answered on Oct 26 2016

    Question

    Which buffer can be used for diluting serum samples?

    Read More

    Abcam community

    Verified customer

    Asked on Oct 10 2016

    Answer

    I can confirm the lab used PBS to dilute serum samples when validating the kit.

    Read More

    Abcam Scientific Support

    Answered on Oct 10 2016

    Question

    I am using undiluted serum and plasma samples and observing high OD values. Do you have any suggestion why is this so?

    Read More

    Abcam community

    Verified customer

    Asked on Sep 01 2016

    Answer

    This is most likely due to matrix effect which affect measurement of analyte of interest in ELISA when undiluted serum or plasma samples are used. Matrix effect can arise from a number of matrix components such as phospholipids, carbohydrates, and endogenous metabolites (bilirubin), residual formulation components from intraperitoneal (ip), intravenous (iv), or oral dosing (po) experiments; for example, polyethylene glycol (PEG), solutol, polysorbate (Tween 80), etc, an interaction between the analyte of interest and the matrix, such as covalent binding to plasma proteins or the enzymatic degradation of a prodrug.  The easiest way to overcome this is by using diluted samples e.g. 1/2 or 1/10 dilution.

    Read More

    Abcam Scientific Support

    Answered on Sep 01 2016

    Question

    Does the assay detect both protein bound and free testosterone? Or is all testosterone considered bound to protein?

    Read More

    Abcam community

    Verified customer

    Asked on Aug 26 2014

    Answer

    The test measures both bound and free Testosterone.

    Read More

    Sybille Rex

    Abcam Scientific Support

    Answered on Aug 26 2014

    1-10 of 14 Abreviews or Q&A

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