Key features and details
- Sensitivity: 0.07 ng/ml
- Range: 0.2 ng/ml - 16 ng/ml
- Sample type: Cit plasma, Hep Plasma, Serum, Urine
- Detection method: Colorimetric
- Assay type: Competitive
- Reacts with: Mouse, Rat, Human
Product nameTestosterone ELISA Kit
See all Testosterone kits
Intra-assay Sample n Mean SD CV% Control Sera 48 <=5.8% Inter-assay Sample n Mean SD CV% Control Sera 48 <=10.5%
Sample typeUrine, Serum, Hep Plasma, Cit plasma
Sensitivity= 0.07 ng/ml
Range0.2 ng/ml - 16 ng/ml
= 105.5 %
Assay durationMultiple steps standard assay
Species reactivityReacts with: Mouse, Rat, Human
Testosterone ELISA kit is a competitive ELISA designed for the accurate quantitative measurement of Testosterone in serum and plasma.
A 96-well plate has been precoated with anti-Testosterone antibodies. Samples and the Testosterone-HRP conjugate are added to the wells, where Testosterone in the sample competes with the added Testosterone-HRP for antibody binding. After incubation, the wells are washed to remove unbound material and TMB substrate is then added which is catalyzed by HRP to produce blue coloration. The reaction is terminated by addition of Stop Solution which stops the color development and produces a color change from blue to yellow. The intensity of signal is inversely proportional to the amount of Testosterone in the sample and the intensity is measured at 450 nm.
Storage instructionsStore at +4°C. Please refer to protocols.
Components 1 x 96 tests 10X Wash Buffer 1 x 50ml Cover foils 1 unit Stop Solution 1 x 15ml Strip holder 1 unit Testosterone conjugate 1 x 12ml Testosterone Control A 1 x 1ml Testosterone Control B 1 x 1ml Testosterone IgG Coated Microtiter Plate (12 x 8 wells) 1 unit Testosterone Standard 0 - 0.0 ng/mL 1 x 1ml Testosterone Standard 1 - 0.2 ng/mL 1 x 1ml Testosterone Standard 2 - 1.0 ng/mL 1 x 1ml Testosterone Standard 3 - 4.0 ng/mL 1 x 1ml Testosterone Standard 4 - 16.0 ng/mL 1 x 1ml TMB Substrate Solution 1 x 15ml
- SwissProt: P04278 Human
ab108666 has been referenced in 35 publications.
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