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We have bought two abcam ELISA kits to dose testosterone (ab108666) and cortisol (ab108665) from human blood samples. Unfortunately, blood samples were frozen without separating blood products (no centrifugation before freezing). As a result, there has been heavy hemolysis in our samples.
Since these samples cannot be taken again, I have two questions: is it still possible to get at least a 'relative' measure of cortisol and/or testosterone or is it absolutely impossible? is there some specific recommendations for our situation? Diluting samples? Centrifuging more to remove big cell components?
Asked on Apr 24 2013
I confirmed with the lab and unfortunately do not have good news:
The heavily hemolyzed samples would have a great influence on the results. We do not know of any protocol trick to circumvent this and therefore, recommend to collect new samples.
But we would assume that all samples would interfere with the assay at the same level.
Answered on Apr 24 2013