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I have a couple of questions regarding this kit. First, is there a way to ascertain free testosterone from this kit as well, or is it necessary to perform a separate ELISA? Second, how many wells on the plate are used for controls?
Asked on Oct 26 2016
This assay will measure total testosterone, free and also protein-bound. To measure just free testosterone, we have another assay, https://www.abcam.com/human-testosterone-free-elisa-kit-ab178663.html.
We recommend running the two controls in duplicate, so those will require 4 wells. The five standards in duplicate will require 10 wells. One more well is needed for the substrate blank.
Answered on Oct 26 2016