Overview

  • Product name

    Testosterone ELISA Kit
    See all Testosterone kits
  • Detection method

    Colorimetric
  • Precision

    Intra-assay
    Sample n Mean SD CV%
    Saliva 16 <=8%
    Inter-assay
    Sample n Mean SD CV%
    Saliva 12 <=13.2%
  • Sample type

    Saliva
  • Assay type

    Competitive
  • Sensitivity

    >= 2.96 pg/ml
  • Range

    10 pg/ml - 210 pg/ml
  • Recovery

    98.97 %

  • Assay duration

    Multiple steps standard assay
  • Species reactivity

    Reacts with: Human
  • Product overview

    Abcam’s Testosterone in vitro competitive ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate quantitative measurement of Testosterone in saliva.

    A 96-well plate has been precoated with anti-Testosterone antibodies (solid-phase). Samples and the Testosterone-HRP conjugate are added to the wells, where Testosterone in the sample competes with the added Testosterone-HRP conjugate for antibody binding. After incubation, the wells are washed to remove unbound material and TMB substrate is then added which is catalyzed by HRP to produce blue coloration. The reaction is terminated by addition of Stop Solution which stops the color development and produces a color change from blue to yellow. The intensity of signal is inversely proportional to the amount of Testosterone in the sample and the intensity is measured at 450 nm.

  • Tested applications

    Suitable for: Competitive ELISAmore details
  • Platform

    Microplate

Properties

Applications

Our Abpromise guarantee covers the use of ab178655 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Competitive ELISA Use at an assay dependent concentration.

Images

  • Representative standard curve using ab178655.

Protocols

References

This product has been referenced in:

  • Ali TM & El Askary A The association between fetuin-A and testosterone levels and markers of arterial stiffness in Saudi subjects with type 2 diabetes mellitus. Diabetes Metab Syndr 12:1045-1050 (2018). Read more (PubMed: 30168427) »
See 1 Publication for this product

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