Overview

  • Product name

    Anti-TET1 antibody - C-terminal
    See all TET1 primary antibodies
  • Description

    Rabbit polyclonal to TET1 - C-terminal
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human TET1 (C terminal). The exact sequence is proprietary. 18 amino acid peptide. The immunogen is located with amino acids 2030 - 2080.
    Database link: Q8NFU7

  • Positive control

    • Western blot: Human, mouse and rat testis; human bladder. IHC-P: Human and mouse testis. ICC/IF: HeLa and EL4 cell lines.

Properties

Applications

Our Abpromise guarantee covers the use of ab191698 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 235 kDa.

Recommend for human and mouse and rat. Please see image legend for details of the Western blot protocol. 

IHC-P Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Recommend for human and mouse. Please see image legends for more protocol details. 

ICC/IF Use a concentration of 20 µg/ml.

Recommend for human and mouse. Please see image legends for more protocol details. 

Target

  • Function

    Dioxygenase that catalyzes the conversion of methylcytosine (5mC) to 5-hydroxymethylcytosine (hmC). Plays a role in embryonic stem (ES) cell maintenance and inner cell mass (ICM) cell specification, possibly by participating in DNA demethylation. Specifically binds 5mC, a minor base in mammalian DNA found in repetitive DNA elements that is crucial for retrotransposon silencing and mammalian development. 5mC is present in ES cells and is enriched in the brain, especially in Purkinje neurons. The clear function of hmC is still unclear but it could constitute an intermediate component in cytosine demethylation. A role of hmC in DNA demethylation is supported by TET1 function in ES cell maintenance, which is required to prevent NANOG hypermethylation and maintain NANOG expression in ES cells.
  • Tissue specificity

    Expressed in fetal heart, lung and brain, and in adult skeletal muscle, thymus and ovary. Not detected in adult heart, lung or brain.
  • Involvement in disease

    Note=A chromosomal aberration involving TET1 may be a cause of acute leukemias. Translocation t(10;11)(q22;q23) with MLL. This is a rare chromosomal translocation 5' MLL-TET1 3'.
  • Sequence similarities

    Belongs to the TET family.
    Contains 1 CXXC-type zinc finger.
  • Cellular localization

    Nucleus.
  • Information by UniProt
  • Database links

  • Alternative names

    • bA119F7.1 antibody
    • CXXC 6 antibody
    • CXXC finger 6 antibody
    • CXXC type zinc finger protein 6 antibody
    • CXXC zinc finger 6 antibody
    • CXXC-type zinc finger protein 6 antibody
    • CXXC6 antibody
    • KIAA1676 antibody
    • LCX antibody
    • Leukemia associated protein with a CXXC domain antibody
    • Leukemia-associated protein with a CXXC domain antibody
    • Methylcytosine dioxygenase TET1 antibody
    • Ten eleven translocation 1 antibody
    • Ten eleven translocation 1 gene protein antibody
    • Ten eleven translocation 1 gene protein homolog antibody
    • Ten-eleven translocation 1 gene protein antibody
    • Tet 1 antibody
    • Tet methylcytosine dioxygenase 1 antibody
    • Tet oncogene 1 antibody
    • TET1 antibody
    • TET1_HUMAN antibody
    see all

Images

  • All lanes : Anti-TET1 antibody - C-terminal (ab191698) at 1 µg/ml

    Lane 1 : Mouse testis
    Lane 2 : Rat testis
    Lane 3 : Human testis
    Lane 4 : Human bladder

    Lysates/proteins at 15 µg per lane.

    Secondary
    All lanes : Goat anti-rabbit IgG HRP conjugate at 1/10000 dilution

    Developed using the ECL technique.

    Predicted band size: 235 kDa
    Additional bands at: 55-72 kDa (possible non-specific binding)


    Exposure time: 1 minute


    8% SDS gel

    Running condition: 130V, 1.5 hours.

    Transfer condition: Wet, 250mA, 2 hrs (Nitrocellulose membrane)

    Blocking condition: 5% non-fat milk in TBS at 4 overnight.

    Primary antibody incubation: room temperature for 1 hour.

    Secondary antibody incubation: room temperature for 1 hour.

    Washing condition: 5 mL TBST, 4 x 5 minutes.

  • Immunohistochemical analysis of paraffin-embedded human testis tissue labeling TET1 with ab191698 at 1 µg/mL (4°C overnight), followed by Streptavidin-HRP Kit (Rabbit IgG) (Room temperature, 1 hour). Nuclear staining on human testis is observed. DAB staining. 

    Antigen Retrieval: Citrate buffer, pH 6.0, microwave, 20 minutes.

    Endogenous peroxidase blocking: 3% H2O2, Room temperature, 10 minutes.

    Blocking condition: 5% BSA, room temperature, 1 hour.

    Washing condition: PBS, 2 X 5 minutes. 

     

  • Immunofluorescent analysis of 4% paraformaldehyde-fixed (room temperature, 10 minutes), 0.1% Triton X-100 in PBS permeabilized (room temperature, 5 minutes) HeLa cells labeling TET1 with ab191698 at 20 µg/mL (incubation at 4°C overnight), followed by Rabbit IgG antibody (Alexa Fluor 488) secondary antibody at 1/2000 dilution (green) (Incubation at room temperature for 1 hour). Confocal image showing nuclear staining on HeLa cell line. The nuclear counter stain is DAPI (blue).

    Blocking condition: 5% BSA, room temperature, 1 hour.

    Washing condition: PBS, 3 x 3 minutes. 

     

  • Immunohistochemical analysis of paraffin-embedded mouse testis tissue labeling TET1 with ab191698 at 1 µg/mL (4°C overnight), followed by Streptavidin-HRP Kit (Rabbit IgG) (Room temperature, 1 hour). Nuclear staining on mouse testis is observed. DAB staining. 

    Antigen Retrieval: Citrate buffer, pH 6.0, microwave, 20 minutes.

    Endogenous peroxidase blocking: 3% H2O2, Room temperature, 10 minutes.

    Blocking condition: 5% BSA, room temperature, 1 hour.

    Washing condition: PBS, 2 X 5 minutes. 

     

  • Immunofluorescent analysis of 4% paraformaldehyde-fixed (room temperature, 10 minutes), 0.1% Triton X-100 in PBS permeabilized (room temperature, 5 minutes) EL4 cells (mouse T-lymphocyte cell line) labeling TET1 with ab191698 at 20 µg/mL (incubation at 4°C overnight), followed by Rabbit IgG antibody (Alexa Fluor 488) secondary antibody at 1/2000 dilution (green) (Incubation at room temperature for 1 hour). Confocal image showing nuclear staining on EL4 cell line. The nuclear counter stain is DAPI (blue).

    Blocking condition: 5% BSA, room temperature, 1 hour.

    Washing condition: PBS, 3 x 3 minutes. 

     

References

This product has been referenced in:

  • Wang C  et al. Enhanced expression of ten-eleven translocation 1 reverses gemcitabine resistance in cholangiocarcinoma accompanied by a reduction in P-glycoprotein expression. Cancer Med 8:990-1003 (2019). Read more (PubMed: 30784212) »
  • Ma M  et al. Preeclampsia is associated with hypermethylation of IGF-1 promoter mediated by DNMT1. Am J Transl Res 10:16-39 (2018). IHC-P ; Human . Read more (PubMed: 29422991) »
See all 12 Publications for this product

Customer reviews and Q&As

1-2 of 2 Abreviews or Q&A

Application
Western blot
Sample
Mouse Tissue lysate - whole (Hippocampus)
Gel Running Conditions
Reduced Denaturing (5)
Loading amount
20 µg
Specification
Hippocampus
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C

Abcam user community

Verified customer

Submitted Apr 20 2017

Application
Western blot
Sample
Mouse Cell lysate - nuclear (mouse embryonic stem cells)
Gel Running Conditions
Reduced Denaturing
Loading amount
20 µg
Specification
mouse embryonic stem cells
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C

Abcam user community

Verified customer

Submitted Mar 16 2016

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