• Product name
  • Description
    Rabbit polyclonal to Tet2
  • Host species
  • Tested applications
    Suitable for: IHC-P, IP, ICC/IF, WBmore details
  • Species reactivity
    Reacts with: Mouse, Human, Common marmoset
    Predicted to work with: Dog
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Human Tet2.

  • Positive control
    • This antibody gave a positive signal in MCF7 whole cell lysate. ICC/IF: MCF7 cells.


  • Form
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: 0.02% Sodium Azide
    Constituents: 1% BSA, PBS, pH 7.4
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
  • Isotype
  • Research areas


Our Abpromise guarantee covers the use of ab94580 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
IP Use at an assay dependent concentration. PubMed: 24077167
ICC/IF Use a concentration of 10 µg/ml.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 224 kDa (predicted molecular weight: 224 kDa). Abcam recommends using milk (2-5%) as the blocking agent.


  • Function
    Catalyzes the conversion of methylcytosine (5mC) to 5-hydroxymethylcytosine (hmC). Plays an important role in myelopoiesis. The clear function of 5-hydroxymethylcytosine (hmC) is still unclear but it may influence chromatin structure and recruit specific factors or may constitute an intermediate component in cytosine demethylation.
  • Tissue specificity
    Broadly expressed. Highly expressed in hematopoietic cells; highest expression observed in granulocytes. Expression is reduced in granulocytes from peripheral blood of patients affected by myelodysplastic syndromes.
  • Involvement in disease
    Note=TET2 is frequently mutated in myeloproliferative disorders (MPD). These constitute a heterogeneous group of disorders, also known as myeloproliferative diseases or myeloproliferative neoplasms (MPN), characterized by cellular proliferation of one or more hematologic cell lines in the peripheral blood, distinct from acute leukemia. Included diseases are: essential thrombocythemia, polycythemia vera, primary myelofibrosis (chronic idiopathic myelofibrosis). Bone marrow samples from patients display uniformly low levels of hmC in genomic DNA compared to bone marrow samples from healthy controls as well as hypomethylation relative to controls at the majority of differentially methylated CpG sites.
    Defects in TET2 are a cause of polycythemia vera (PV) [MIM:263300]. A myeloproliferative disorder characterized by abnormal proliferation of all hematopoietic bone marrow elements, erythroid hyperplasia, an absolute increase in total blood volume, but also by myeloid leukocytosis, thrombocytosis and splenomegaly.
    Note=TET2 is frequently mutated in systemic mastocytosis; also known as systemic mast cell disease. A condition with features in common with myeloproliferative diseases. It is a clonal disorder of the mast cell and its precursor cells. The clinical symptoms and signs of systemic mastocytosis are due to accumulation of clonally derived mast cells in different tissues, including bone marrow, skin, the gastrointestinal tract, the liver, and the spleen.
    Note=TET2 is frequently mutated in myelodysplastic syndromes, a heterogeneous group of closely related clonal hematopoietic disorders. All are characterized by a hypercellular or hypocellular bone marrow with impaired morphology and maturation, dysplasia of the myeloid, megakaryocytic and/or erythroid lineages, and peripheral blood cytopenias resulting from ineffective blood cell production. Included diseases are: refractory anemia (RA), refractory anemia with ringed sideroblasts (RARS), refractory anemia with excess blasts (RAEB), refractory cytopenia with multilineage dysplasia and ringed sideroblasts (RCMD-RS). Chronic myelomonocytic leukemia (CMML) is a myelodysplastic/myeloproliferative disease. Myelodysplastic syndromes are considered a premalignant condition in a subgroup of patients that often progresses to acute myeloid leukemia (AML). Bone marrow samples from patients display uniformly low levels of hmC in genomic DNA compared to bone marrow samples from healthy controls as well as hypomethylation relative to controls at the majority of differentially methylated CpG sites.
  • Sequence similarities
    Belongs to the TET family.
  • Information by UniProt
  • Database links
  • Alternative names
    • FLJ20032 antibody
    • KIAA1546 antibody
    • MDS antibody
    • Methylcytosine dioxygenase TET2 antibody
    • Nbla00191 antibody
    • Probable methylcytosine dioxygenase TET2 antibody
    • Protein Ayu17 449 antibody
    • Tet 2 antibody
    • Tet methylcytosine dioxygenase 2 antibody
    • Tet oncogene 2 antibody
    • Tet oncogene family member 2 antibody
    • TET2 antibody
    • TET2_HUMAN antibody
    see all


  • Lane 1: Wild-type HAP1 whole cell lysate (20 µg)
    Lane 2: Tet2 knockout HAP1 whole cell lysate (20 µg)
    Lane 3: HeLa whole cell lysate (20 µg)
    Lane 4: F9 whole cell lysate (20 µg) 

    Lanes 1 - 4: Merged signal (red and green). Green - ab94580 observed at 250 kDa. Red - loading control, ab18058, observed at 130 kDa. 

    ab94580 was shown to specifically recognize Tet2 in wild-type HAP1 cells along with additional cross-reactive bands. No band was observed when Tet2 knockout samples were examined. Wild-type and Tet2 knockout samples were subjected to SDS-PAGE. Ab94580 and ab18058 (Mouse anti-Vinculin loading control) were incubated overnight at 4°C at 1 µg/ml and 1/10,000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/10,000 dilution for 1 hour at room temperature before imaging.

  • Lane 1 : Anti-Tet2 antibody (ab94580) at 1 µg/ml (blocked with 5% BSA)
    Lane 2 : Anti-Tet2 antibody (ab94580) at 1 µg/ml (blocked with 5% milk)
    Lane 3 : Anti-Tet2 antibody (ab94580) at 1 µg/ml (blocked with 2% milk)

    All lanes : MCF7 (Human breast adenocarcinoma cell line) Whole Cell Lysate

    Lysates/proteins at 20 µg per lane.

    All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 224 kDa
    Observed band size: 224 kDa
    Additional bands at: 300 kDa, 55 kDa, 70 kDa. We are unsure as to the identity of these extra bands.

    Exposure time: 20 minutes

    Based on this data we recommend using milk as the blocking agent. We welcome customer feedback and would appreciate any comments regarding this product and the data presented above.

  • ICC/IF image of ab94580 stained MCF-7 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab94580 at 10µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. This antibody also gave a positive result in HeLa pfa fixed (4%, 10 minutes) cell types at 10ug/ml.

  • ab94580 staining Tet2 in Marmoset adult testis tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 5% milk for 30 minutes at 25°C; antigen retrieval was by heat mediation in Dako antigen retrieval solution. Samples were incubated with primary antibody (1/100 in 5% milk) for 18 hours at 4°C. An Alexa Fluor® 555-conjugated Goat anti-rabbit polyclonal (1/500) was used as the secondary antibody.

    See Abreview


This product has been referenced in:
  • Ma M  et al. Preeclampsia is associated with hypermethylation of IGF-1 promoter mediated by DNMT1. Am J Transl Res 10:16-39 (2018). IHC-P ; Human . Read more (PubMed: 29422991) »
  • Yang R  et al. Tet1 and Tet2 maintain mesenchymal stem cell homeostasis via demethylation of the P2rX7 promoter. Nat Commun 9:2143 (2018). Read more (PubMed: 29858571) »

See all 15 Publications for this product

Customer reviews and Q&As

Western blot
Rat Cell lysate - whole cell (HEK and NRK)
Gel Running Conditions
Reduced Denaturing (6)
Loading amount
20 µg
Blocking step
(agent) for 1 hour(s) and 0 minute(s) · Concentration: 5µg/mL · Temperature: RT°C

Abcam user community

Verified customer

Submitted Apr 28 2017

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Dako antigen retrieval solution
Marmoset (common) Tissue sections (Adult testis)
Adult testis

Dr. 煜欽 林

Verified customer

Submitted Jan 20 2014

Thank you for sharing those results. It is disappointing to hear that this new lot has not provided a better result.
I have raised this problem with you lab and quality control team. We are currently trying to ascertain why this change in reactivi...

Read More
Abcam guarantees this product to work in the species/application used in this Abreview.
Immunocytochemistry/ Immunofluorescence
Mouse Cell (mouse pluripotent cells)
mouse pluripotent cells
Yes - 0.25% Triton X-100
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 37°C

Abcam user community

Verified customer

Submitted Nov 02 2012

I am sorry for the delay in getting back to you.
I can now confirm that we do have a different lot of ab94580 in stock which has been tested in both Western blotting and IF. If you would like I can arrange to send this to you. In order to arrange ...

Read More

Thank you very much for that reply.
That information has been very helpful in looking into this case further. I am sorry that the particular lot has not been producing the expected results.
I am currently looking into whether we have a differ...

Read More

Thank you for contacting us and reporting the problems you have been encountering with the Anti-Tet2 antibody (ab94580).
I am sorry to hear you are experiencing difficulties with one of our products. We take product complaints very seriously, and ...

Read More

Thank you for contacting Abcam.

I am sorry about the issues you have been having with ab94580 in western blot. The antibody is covered under our Abpromise for six months and is guaranteed to work in western blot on mouse & humansamples. I...

Read More

Thank you very much for your reply.

That will not be a problem. This credit will not be refunded unlessyou contact ouraccounting department to request the refund,so the credit can be kept on your account andredeemed against the invoice of a f...

Read More

Thank you for contacting us and letting us know about the trouble with these antibodies.

Thank you also for sending the information about your protocol and the image. Both of these antibodies may need to be used at lower dilutions than 1:2000...

Read More

1-10 of 18 Abreviews or Q&A


Sign up