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  1. Link

    tet2-antibody-epr20546-135-ab213369.pdf

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Epigenetics and Nuclear Signaling DNA methylation Methylated DNA Associated
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Validated using a knockout cell lineRecombinantRabMAb

Recombinant Anti-Tet2 antibody [EPR20546-135] (ab213369)

  • Datasheet
  • Certificate of Compliance
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Western blot - Anti-Tet2 antibody [EPR20546-135] (ab213369)
  • Western blot - Anti-Tet2 antibody [EPR20546-135] (ab213369)
  • Western blot - Anti-Tet2 antibody [EPR20546-135] (ab213369)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR20546-135] to Tet2
  • Suitable for: WB
  • Knockout validated
  • Reacts with: Mouse

Conjugates logo Related conjugates and formulations

Carrier Free

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Overview

  • Product name

    Anti-Tet2 antibody [EPR20546-135]
    See all Tet2 primary antibodies
  • Description

    Rabbit monoclonal [EPR20546-135] to Tet2
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WBmore details
    Unsuitable for: Flow Cyt (Intra),ICC,IHC-P or IP
  • Species reactivity

    Reacts with: Mouse
  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: WT mESCs, RAW264.7 (mouse Abelson murine leukemia virus-induced tumor macrophage), whole cell lysate; Wild-type B-cell lysate.
  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.01% Sodium azide
    Constituents: 59.94% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR20546-135
  • Isotype

    IgG
  • Research areas

    • Epigenetics and Nuclear Signaling
    • DNA methylation
    • Methylated DNA Associated

Associated products

  • Alternative Versions

    • Anti-Tet2 antibody [EPR20546-135] - BSA and Azide free (ab277637)
  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (HRP) (ab97051)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab213369 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB (1)
1/1000. Predicted molecular weight: 223 kDa.
Notes
WB
1/1000. Predicted molecular weight: 223 kDa.
Application notes
Is unsuitable for Flow Cyt (Intra),ICC,IHC-P or IP.

Target

  • Function

    Catalyzes the conversion of methylcytosine (5mC) to 5-hydroxymethylcytosine (hmC). Plays an important role in myelopoiesis. The clear function of 5-hydroxymethylcytosine (hmC) is still unclear but it may influence chromatin structure and recruit specific factors or may constitute an intermediate component in cytosine demethylation.
  • Tissue specificity

    Broadly expressed. Highly expressed in hematopoietic cells; highest expression observed in granulocytes. Expression is reduced in granulocytes from peripheral blood of patients affected by myelodysplastic syndromes.
  • Involvement in disease

    Note=TET2 is frequently mutated in myeloproliferative disorders (MPD). These constitute a heterogeneous group of disorders, also known as myeloproliferative diseases or myeloproliferative neoplasms (MPN), characterized by cellular proliferation of one or more hematologic cell lines in the peripheral blood, distinct from acute leukemia. Included diseases are: essential thrombocythemia, polycythemia vera, primary myelofibrosis (chronic idiopathic myelofibrosis). Bone marrow samples from patients display uniformly low levels of hmC in genomic DNA compared to bone marrow samples from healthy controls as well as hypomethylation relative to controls at the majority of differentially methylated CpG sites.
    Defects in TET2 are a cause of polycythemia vera (PV) [MIM:263300]. A myeloproliferative disorder characterized by abnormal proliferation of all hematopoietic bone marrow elements, erythroid hyperplasia, an absolute increase in total blood volume, but also by myeloid leukocytosis, thrombocytosis and splenomegaly.
    Note=TET2 is frequently mutated in systemic mastocytosis; also known as systemic mast cell disease. A condition with features in common with myeloproliferative diseases. It is a clonal disorder of the mast cell and its precursor cells. The clinical symptoms and signs of systemic mastocytosis are due to accumulation of clonally derived mast cells in different tissues, including bone marrow, skin, the gastrointestinal tract, the liver, and the spleen.
    Note=TET2 is frequently mutated in myelodysplastic syndromes, a heterogeneous group of closely related clonal hematopoietic disorders. All are characterized by a hypercellular or hypocellular bone marrow with impaired morphology and maturation, dysplasia of the myeloid, megakaryocytic and/or erythroid lineages, and peripheral blood cytopenias resulting from ineffective blood cell production. Included diseases are: refractory anemia (RA), refractory anemia with ringed sideroblasts (RARS), refractory anemia with excess blasts (RAEB), refractory cytopenia with multilineage dysplasia and ringed sideroblasts (RCMD-RS). Chronic myelomonocytic leukemia (CMML) is a myelodysplastic/myeloproliferative disease. Myelodysplastic syndromes are considered a premalignant condition in a subgroup of patients that often progresses to acute myeloid leukemia (AML). Bone marrow samples from patients display uniformly low levels of hmC in genomic DNA compared to bone marrow samples from healthy controls as well as hypomethylation relative to controls at the majority of differentially methylated CpG sites.
  • Sequence similarities

    Belongs to the TET family.
  • Target information above from: UniProt accession Q6N021 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 214133 Mouse
    • SwissProt: Q4JK59 Mouse
    • Unigene: 347816 Mouse
    • Alternative names

      • FLJ20032 antibody
      • KIAA1546 antibody
      • MDS antibody
      • Methylcytosine dioxygenase TET2 antibody
      • Nbla00191 antibody
      • Probable methylcytosine dioxygenase TET2 antibody
      • Protein Ayu17 449 antibody
      • Tet 2 antibody
      • Tet methylcytosine dioxygenase 2 antibody
      • Tet oncogene 2 antibody
      • Tet oncogene family member 2 antibody
      • TET2 antibody
      • TET2_HUMAN antibody
      see all

    Images

    • Western blot - Anti-Tet2 antibody [EPR20546-135] (ab213369)
      Western blot - Anti-Tet2 antibody [EPR20546-135] (ab213369)Image courtesy of Dr Hugo Sepulveda in Prof Anjana Rao’s laboratory (La Jolla Institute for Immunology)
      All lanes : Anti-Tet2 antibody [EPR20546-135] (ab213369) at 1/2000 dilution

      Lane 1 : Wild-Type Mouse embryonic stem cells (mESCs)
      Lane 2 : Tet1/2/3 Triple-Knockout (Tet-TKOs) Mouse embryonic stem cells (mESCs)

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Rat anti-mouse HRP Conjugate at 1/4000 dilution

      Predicted band size: 223 kDa
      Observed band size: 250 kDa why is the actual band size different from the predicted?
      Additional bands at: 65 kDa (possible non-specific binding)


      Exposure time: 10 seconds


      Blocking buffer: 5% milk.

      See Abreview

    • Western blot - Anti-Tet2 antibody [EPR20546-135] (ab213369)
      Western blot - Anti-Tet2 antibody [EPR20546-135] (ab213369)Image courtesy of Dr Hugo Sepulveda in Prof Anjana Rao’s laboratory (La Jolla Institute for Immunology)
      All lanes : Anti-Tet2 antibody [EPR20546-135] (ab213369) at 1/1000 dilution

      Lane 1 : Wild-type B-cell lysate
      Lane 2 : Tet2/3 DKO B-cell lysate

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution (Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated)

      Predicted band size: 223 kDa
      Observed band size: 257 kDa why is the actual band size different from the predicted?


      Exposure time: 60 seconds


      Blocking and Diluting buffer: 5% NFDM/TBST

      This western was done using lysates from a Tet2/3 DKO B-cell model developed by Dr Vipul Shukla and Dr Chan-Wang Lio, in Prof Anjana Rao’s lab.

    • Western blot - Anti-Tet2 antibody [EPR20546-135] (ab213369)
      Western blot - Anti-Tet2 antibody [EPR20546-135] (ab213369)
      Anti-Tet2 antibody [EPR20546-135] (ab213369) at 1/1000 dilution + RAW264.7 (mouse Abelson murine leukemia virus-induced tumor macrophage), whole cell lysate at 20 µg

      Secondary
      Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution (Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated)

      Predicted band size: 223 kDa
      Observed band size: 257 kDa why is the actual band size different from the predicted?



      Blocking and Diluting buffer: 5% NFDM/TBST

      Exposure time: 3 minutes

      This blot was developed using a higher sensitivity ECL substrate.

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    Datasheets and documents

    • Datasheet download

      Download

    Certificate of Compliance

    To download a Certificate of Compliance, please enter your Lot number below:

    References (0)

    Publishing research using ab213369? Please let us know so that we can cite the reference in this datasheet.

    ab213369 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    Western blot abreview for Anti-Tet2 antibody [EPR20546-135]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Mouse Cell lysate - whole cell (mESCs)
    Gel Running Conditions
    Reduced Denaturing (Gradient polyacrylamide gel)
    Loading amount
    20 µg
    Treatment
    WTs vs KO
    Specification
    mESCs
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 16°C
    Read More

    Hugo Sepulveda Inostroza

    Verified customer

    Submitted Apr 14 2021

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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