Overview

  • Product name

  • Description

    Rabbit polyclonal to TET3
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IPmore details
  • Species reactivity

    Reacts with: Mouse, Human
  • Immunogen

    Recombinant fragment containing the N terminal region of Human TET3 with proprietary tag.

  • Positive control

    • Human Jurkat cell extract and Mouse thymus cell extract.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at -20ºC.
  • Storage buffer

    pH: 7.40
    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol, 0.01% BSA
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab139311 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/2000. Predicted molecular weight: 179 kDa.
IP 1/50 - 1/100.

Target

Images

  • Total extracts from mouse thymus were immunoprecipitated with an anti-TET2 antibody (Lane 1) or ab139311 (Lane 2) at 1/50 dilution, followed by Western Blot analysis using ab139311 at 1/500 dilution for 2 hours at RT.

  • All lanes : Anti-TET3 antibody (ab139311) at 1/500 dilution

    Lane 1 : Human Jurkat cell extract
    Lane 2 : Mouse thymus cell extract

    Predicted band size: 179 kDa

References

This product has been referenced in:

  • Liu Z  et al. Melatonin alleviates adipose inflammation through elevating a-ketoglutarate and diverting adipose-derived exosomes to macrophages in mice. J Pineal Res 64:N/A (2018). Read more (PubMed: 29149454) »
  • Zhao S  et al. Reduced mRNA and Protein Expression Levels of Tet Methylcytosine Dioxygenase 3 in Endothelial Progenitor Cells of Patients of Type 2 Diabetes With Peripheral Artery Disease. Front Immunol 9:2859 (2018). Read more (PubMed: 30574144) »
See all 5 Publications for this product

Customer reviews and Q&As

1-4 of 4 Abreviews or Q&A

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (small intestine)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: EDTA/Citrate
Permeabilization
No
Specification
small intestine
Fixative
Formaldehyde

Miss. Meshi Danino

Verified customer

Submitted Jan 07 2019

Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (E10.5 embryo)
Permeabilization
Yes - 0.1% Triton
Specification
E10.5 embryo
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C
Fixative
Paraformaldehyde

Abcam user community

Verified customer

Submitted Mar 04 2016

ChIP

Poor
Abreviews
Application
ChIP
Detection step
Real-time PCR
Sample
Human Cell lysate - whole cell (HEK293T cells)
Specification
HEK293T cells
Negative control
As negative genomic controls, I have analized two genomic regions called: "intergenic" and PPIH in which it was demostrated that TET3 is not able to bind on it.(DePlus R. et al, EMBO J. 2013 Mar 632(5):645-55)
Type
Cross-linking (X-ChIP)
Duration of cross-linking step: 10 minute(s) and 0 second(s)
Specification of the cross-linking agent: formaldehyde
Positive control
As positive genomic controls, I have analized the transcription start sites of 3 genes: TAF12, ZNF335, LRIG2 in which it was demostrated TET3 binding. (DePlus R. et al, EMBO J. 2013 Mar 632(5):645-55)

Abcam user community

Verified customer

Submitted Jun 07 2013

Question
Answer


Since ab139311 is a polyclonal antibody, it is standardized by relative signal on Western blot since there are multiple proteins that recognize different immunogens on the antigen as opposed to monoclonal antibodies which contain copies of the same antibody that recognizes one immunogen on the antigen.

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