Key features and details
- Rabbit polyclonal to TET3
- Suitable for: WB, ICC/IF, IP
- Reacts with: Mouse, Human
- Isotype: IgG
Product nameAnti-TET3 antibody
See all TET3 primary antibodies
DescriptionRabbit polyclonal to TET3
Tested applicationsSuitable for: WB, ICC/IF, IPmore details
Species reactivityReacts with: Mouse, Human
Recombinant fragment, corresponding to a region within amino acids 1456-1660 of Human TET3 (Uniprot ID: O43151).
- IHC-P: Mouse colon tissue; IF: HeLa cells; IP: Transfected HEK-293T cells.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.00
Preservative: 0.025% Proclin 300
Constituents: PBS, 20% Glycerol
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab153724 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/10000. Predicted molecular weight: 179 kDa.|
|ICC/IF||1/100 - 1/1000.|
|IP||1/500 - 1/1000.|
FunctionCatalyzes the conversion of methylcytosine (5mC) to 5-hydroxymethylcytosine (hmC).
Tissue specificityExpressed in colon, muscle, adrenal gland and peripheral blood lymphocytes.
Sequence similaritiesBelongs to the TET family.
Developmental stageExpressed in fetal brain but not adult brain.
- Information by UniProt
- hCG_40738 antibody
- KIAA0401 antibody
- Methylcytosine dioxygenase TET3 antibody
Immunohistochemical analysis of paraffin-embedded mouse colon tissue staining TET3 protein at nucleus with ab153724 at 1/500 dilution.
Antigen Retrieval: EDTA based buffer, 15min.
Immunofluorescence analysis of 4% paraformaldehyde-fixed (RT for 15 min) HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate labeling TET3 protein at cytoplasm and nucleus (green) with ab153724 at 1/400 dilution. Red: phalloidin, a cytoskeleton marker, at 1/50 dilution.
Immunoprecipitation of TET3-transfected HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) cells.
Lane 1: Transfected HEK-293T cell lysate.
Lane 2: Control with 2 µg of preimmune rabbit IgG
Lane 3: Immunoprecipitate of TET3-transfected HEK-293T cells using ab153724 at 2 µg.
Detection using ab153724 at 1/1000 dilution, followed by anti-rabbit IgG antibody.
ab153724 has not yet been referenced specifically in any publications.