Product nameAnti-TET3 antibody - C-terminal
See all TET3 primary antibodies
DescriptionRabbit polyclonal to TET3 - C-terminal
Tested applicationsSuitable for: WB, ICC/IF, IPmore details
Species reactivityReacts with: Human
Recombinant fragment, corresponding to a region within amino acids 1456-1660 of Human TET3 (Uniprot ID: O43151).
- Human TET3-transfected 293T cell lysate with DDDDK tag; HeLa cells.
Storage instructionsShipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: PBS, 20% Glycerol
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab153724 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/10000. Predicted molecular weight: 179 kDa.|
|ICC/IF||1/100 - 1/1000.|
|IP||1/500 - 1/1000.|
FunctionCatalyzes the conversion of methylcytosine (5mC) to 5-hydroxymethylcytosine (hmC).
Tissue specificityExpressed in colon, muscle, adrenal gland and peripheral blood lymphocytes.
Sequence similaritiesBelongs to the TET family.
Developmental stageExpressed in fetal brain but not adult brain.
- Information by UniProt
- hCG_40738 antibody
- KIAA0401 antibody
- Methylcytosine dioxygenase TET3 antibody
All lanes : Anti-TET3 antibody - C-terminal (ab153724) at 1/5000 dilution
Lane 1 : 293T whole cell lysate
Lane 2 : Human TET3-transfected 293T whole cell lysate with DDDDK tag
Lysates/proteins at 30 µg per lane.
Predicted band size: 179 kDa
5 % SDS-PAGE
Detection of TET3 in Immunoprecipitate of TET3-transfected 293T cells.
Lane 1: Transfected 293T cell lysate.
Lane 2: Control with 2 µg of preimmune rabbit IgG
Lane 3: Immunoprecipitate of TET3-transfected 293T cells using ab153724 at 2 µg.
Detection using ab153724 at 1/1000 dilution.
All lanes : Anti-TET3 antibody - C-terminal (ab153724) at 1/1000 dilution
Lane 1 : Cell lysate/extract of transfected 293T cells.
Lane 2 : Control of preimmune Rabbit IgG at 2 µg
Lane 3 : Immunoprecipitation of TET3 by ab153724
at 2 µg
Immunofluorescent analysis of paraformaldehyde-fixed HeLa cells, labeling TET3 with ab153724 at 1/500 dilution. Lower image shows cells co-stained with Hoechst 33342.
ab153724 has not yet been referenced specifically in any publications.