Anti-Tetanus Toxin antibody (ab53829)

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Key features and details

  • Rabbit polyclonal to Tetanus Toxin
  • Suitable for: WB, ELISA
  • Isotype: IgG

Overview

  • Product name

    Anti-Tetanus Toxin antibody
    See all Tetanus Toxin primary antibodies

  • Description

    Rabbit polyclonal to Tetanus Toxin

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, ELISAmore details

  • Species reactivity

    Reacts with: Other species

  • Immunogen

    Tetanus toxoid (formaldehyde inactivated tetanus toxin).

  • General notes

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Properties

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab53829 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
Use at an assay dependent dilution. Predicted molecular weight: 150 kDa.
ELISA
Use at an assay dependent dilution.
Notes
WB
Use at an assay dependent dilution. Predicted molecular weight: 150 kDa.
ELISA
Use at an assay dependent dilution.

Target

  • Relevance

    Tetanus toxin acts by inhibiting neurotransmitter release. It binds to peripheral neuronal synapses, is internalized and moves by retrograde transport up the axon into the spinal cord where it can move between postsynaptic and presynaptic neurons. It inhibits neurotransmitter release by acting as a zinc endopeptidase that catalyzes the hydrolysis of the 76-Gln-|-Phe-77 bond of synaptobrevin-2. The precursor polypeptide is subsequently cleaved to yield subchains L and H. These remain linked by a disulfide bridge and are non-toxic after separation.

  • Cellular localization

    Secreted

  • Alternative names

    • Tentoxylysin antibody
    • Tetanus toxin chain H antibody
    • Tetanus toxin chain L antibody
    • Tetanus toxin heavy chain antibody
    • Tetanus toxin light chain antibody
    see all

References (1)

Publishing research using ab53829? Please let us know so that we can cite the reference in this datasheet.

ab53829 has been referenced in 1 publication.

  • Mittal AM  et al. Multiple network properties overcome random connectivity to enable stereotypic sensory responses. Nat Commun 11:1023 (2020). PubMed: 32094345

Customer reviews and Q&As

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1-3 of 3 Abreviews or Q&A

Question

I am going to do WB against recombinant universal T-helper cell epitope, callad tt830-844: a.a. qyikanskfigite. I think this three Ab (ab53829, ab68494 and ab35171) can work. Can you confirm if you used the whole protein to induce the imune response? If these are not suitable can you please recommend one.

Please let me know if you require any further information.

Thanks

Kind regards,

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Answer

Thank you for your enquiry.

The immunogen information is published on each of the product datasheets unless it is a commercially sensitive information. Could I advise you and your customer to read the on-line documents to check the tested applications as well.

ab35171: Synthetic peptide conjugated to KLH, corresponding to amino acids 1300-1314 of Tetanus Toxoid (Clostridium Tetani).

ab53829: Tetanus toxoid (formaldehyde inactivated tetanus toxin).

ab68494: Purified toxin.

These tree antibodies are polyclonals so they recognize multiple epitopes. Therefore, fo rpolyclonals epitop mappig is not performed. If the epitope sequence for monoclonal antibodies are mapped then the information is listed on the datasheets under the "Epitope" section.

I hope this will be useful for you.

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Question

MSDS for ab53829.

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Answer

Thank you for calling Abcam. Please find attached the MSDS for ab53829. If there is anything else I can help you with, please let me kn

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Question

I have a customer with the following questions: Item 1 - Ab53589 Goat polyclonal to Tetanus Toxin. - Does this antibody react with the entire toxin or just the C-fragment? Item 2. Ab53829 Rabbit polyclonal to Tetanus Toxin. - What concentration is recommended for WB and ELISA? (not listed on data sheet - but would it be similar to the ab53589?) - I realise they are different concentrations.

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Answer

Thank you for your enquiry. I can confirm that ab53589 can detect the tetanus toxin which is produced by the soil micro-organism Clostridium tetani, and also the C-fragment of tetanus toxin. Meanwhile, for ab53829 ELISA and WB recommend starting dilution, please try 1/500. However, please bear in mind that the optimal dilutions/concentrations should be determined by the end user. I hope this information will be helpful. If there is anything else that I can help you with, please do not hesitate to contact me.

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