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    tetanus-toxin-antibody-th-11-ab278064.pdf

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Microbiology Toxin Bacterial Toxin Clostridium Toxin
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Anti-Tetanus Toxin antibody [TH-11] (ab278064)

  • Datasheet
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Western blot - Anti-Tetanus Toxin antibody [TH-11] (ab278064)

    Key features and details

    • Mouse monoclonal [TH-11] to Tetanus Toxin
    • Suitable for: WB
    • Isotype: IgG1

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    Overview

    • Product name

      Anti-Tetanus Toxin antibody [TH-11]
      See all Tetanus Toxin primary antibodies
    • Description

      Mouse monoclonal [TH-11] to Tetanus Toxin
    • Host species

      Mouse
    • Tested applications

      Suitable for: WBmore details
    • Immunogen

      Tissue, cells or virus corresponding to Tetanus Toxin. Clostridium tetani Harvard vaccine strain
      Database link: P04958

    • Positive control

      • WB:Tetanus Toxin.

    Properties

    • Form

      Liquid
    • Storage instructions

      Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
    • Storage buffer

      pH: 5
      Constituents: 50% Glycerol (glycerin, glycerine), 50% PBS
    • Concentration information loading...
    • Purity

      Protein G purified
    • Clonality

      Monoclonal
    • Clone number

      TH-11
    • Isotype

      IgG1
    • Light chain type

      kappa
    • Research areas

      • Microbiology
      • Toxin
      • Bacterial Toxin
      • Clostridium Toxin
      • Neuroscience
      • Neurology process
      • Neurodegenerative disease
      • Other

    Associated products

    • Compatible Secondaries

      • Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150113)
      • Goat Anti-Mouse IgG H&L (HRP) (ab205719)

    Applications

    The Abpromise guarantee

    Our Abpromise guarantee covers the use of ab278064 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    WB
    1/1000.
    Notes
    WB
    1/1000.

    Target

    • Relevance

      Tetanus toxin acts by inhibiting neurotransmitter release. It binds to peripheral neuronal synapses, is internalized and moves by retrograde transport up the axon into the spinal cord where it can move between postsynaptic and presynaptic neurons. It inhibits neurotransmitter release by acting as a zinc endopeptidase that catalyzes the hydrolysis of the 76-Gln-|-Phe-77 bond of synaptobrevin-2. The precursor polypeptide is subsequently cleaved to yield subchains L and H. These remain linked by a disulfide bridge and are non-toxic after separation.
    • Cellular localization

      Secreted
    • Alternative names

      • Tentoxylysin antibody
      • Tetanus toxin chain H antibody
      • Tetanus toxin chain L antibody
      • Tetanus toxin heavy chain antibody
      • Tetanus toxin light chain antibody
      see all

    Images

    • Western blot - Anti-Tetanus Toxin antibody [TH-11] (ab278064)
      Western blot - Anti-Tetanus Toxin antibody [TH-11] (ab278064)
      All lanes : Anti-Tetanus Toxin antibody [TH-11] (ab278064) at 1/1000 dilution

      Lane 1 : Reduced tetanus toxin
      Lane 2 : Un-reduced tetanus toxin


      Tetanus toxin Heavy (H) and Light (L) chains

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    Datasheets and documents

    • Datasheet download

      Download

    References (0)

    Publishing research using ab278064? Please let us know so that we can cite the reference in this datasheet.

    ab278064 has not yet been referenced specifically in any publications.

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