Anti-Tetanus Toxoid antibody [5.1B8] (ab26247)

Submit a review Q&A (5)References (4)

Key features and details

  • Mouse monoclonal [5.1B8] to Tetanus Toxoid
  • Suitable for: ELISA
  • Reacts with: Species independent
  • Isotype: IgG1

Overview

  • Product name

    Anti-Tetanus Toxoid antibody [5.1B8]
    See all Tetanus Toxoid primary antibodies

  • Description

    Mouse monoclonal [5.1B8] to Tetanus Toxoid

  • Host species

    Mouse

  • Specificity

    This antibody is specific for tetanus toxin and tetanus toxoid.

  • Tested applications

    Suitable for: ELISAmore details

  • Species reactivity

    Reacts with: Species independent

  • Immunogen

    Full length protein corresponding to Tetanus Toxoid. (formaldehyde inactivated tetanus toxin)

  • General notes


    C. tetani toxoid injected in mice is not neutralized efficiently enough by administration of this antibody to allow rescue.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.

  • Storage buffer

    pH: 7.40
    Preservative: 0.097% Sodium azide
    Constituents: 0.0268% PBS, 2.9% Sodium chloride
  • Concentration information loading...

  • Purification notes

    Protein A/G purified

  • Primary antibody notes

    C. tetani toxoid injected in mice is not neutralized efficiently enough by administration of this antibody to allow rescue.

  • Clonality

    Monoclonal

  • Clone number

    5.1B8

  • Myeloma

    x63-Ag8.653

  • Isotype

    IgG1

  • Light chain type

    kappa

  • Research areas

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab26247 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA
1/12500.
Notes
ELISA
1/12500.

Target

  • Relevance

    Tetanus toxoid is derived from the toxin released by Clostridium Tetanis that causes the disease tetanus. It is used as a vaccine to prevent tetanus or to help boost the immune response to other vaccines. A toxoid is a substance that has been treated to destroy its toxic properties but that retains the capacity to stimulate production of antitoxins, used in immunization. Tetanus toxoid is produced by formaldehyde inactivation of pure neuro toxin. Each lot is verified to be non toxic. In addition to being a potent antigen, tetanus toxoid is a useful carrier protein for making polysaccharides and haptens immunogenic.

  • Cellular localization

    Secreted

  • Alternative names

    • Clostridium tetani toxoid antibody
    • Tentoxylysin antibody
    • Tetanus toxin antibody
    • Tetanus toxin tetX antibody
    • tetX antibody
    see all

Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

References (4)

Publishing research using ab26247? Please let us know so that we can cite the reference in this datasheet.

ab26247 has been referenced in 4 publications.

  • Goyette-Desjardins G  et al. Protection against Streptococcus suis Serotype 2 Infection Using a Capsular Polysaccharide Glycoconjugate Vaccine. Infect Immun 84:2059-75 (2016). PubMed: 27113360
  • Jalah R  et al. Efficacy, but not antibody titer or affinity, of a heroin hapten conjugate vaccine correlates with increasing hapten densities on tetanus toxoid, but not on CRM197 carriers. Bioconjug Chem 26:1041-53 (2015). ELISA ; Mouse . PubMed: 25970207
  • Bertho JM  et al. Influence on the mouse immune system of chronic ingestion of (137)Cs. J Radiol Prot 31:25-39 (2011). ELISA . PubMed: 21346294
  • Peachman KK  et al. The importance of antibody isotype in HIV-1 virus capture assay and in TZM-bl neutralization. Viral Immunol 23:627-32 (2010). PubMed: 21142448

Customer reviews and Q&As

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1-5 of 5 Abreviews or Q&A

Question

is the antibody epitope known? The immunogen is listed as full length protein. I have used this antibody for Western blotting and it detected full size protein wonderfully

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Answer

As far as we know the epitope detected by ab26247 has not yet been determined.

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Question

 Recommended storage temperature for this antibody is 4ºC. Is the same temperature optimal after diluting it in PBS? Would be ultra freezing (-40ºC to -80ºC) harmful to the diluted antibody? Many thanks in advance. Kind regards,  

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Answer

Thank you for contacting us.   We have recommended storing this antibody at 4C. Our Abpromise is only valid if the products are stored correctly as stated on the datasheet. We have not particularly tested the effect of freezing on ab26247 so I am sorry we will not be able to confirm the 100% viability of this antibody after freezing. Normally antibodies are OK to freeze if the cryo-protective agent is present in buffer e.g. BSA or Glycerol. I may suggest adding Glycerol at 1:1 ratio before storing this antibody at -40 or -80C. Please also avoid any unnecessary freeze thawing steps. The antibodies shows better results when stored in non-diluted form.  I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.  

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Question

Is it possible to buy a larger size of this antibody rather than 100ug? Are you able to offer any kind of discount for bulk orders? Thank you!

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Answer

Thank you for your inquiry. I am happy to confirm that we do offer bulk discounts. They start if you order five units (5 x 100ug) with a 5% discount. If you would like a much bigger size, e.g. 1mg or more we might be able to customize the size for you. Please let me know what amount of ab26247 you are interested in and I will get you in contact with the person responsible. I am looking forward to your reply.

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Question

Hi I'm just wondering - this antibody was produced using Tetanus toxoid as the immunogen. Do you sell this toxoid commercially or know of anywhere to obtain it? I've been using this antibody with success over the past few months and I'm happy with it, and so would really like to get hold of some commercially available toxoid to use in the test I've been developing.

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Answer

Thank you for your inquiry. Unfortunately, we do not have any Tetanus toxoid protein available in our catalog. I would like to recommend checking the Biocompare website which has an excellent antibody search facility that includes many suppliers. The links are: www.biocompare.com http://www.biocompare.com/ProductCategories/2045/Antibodies.html I am sorry we are not able to provide what you require on this occasion, but I hope this information is nevertheless helpful to you. Please do not hesitate to contact me if you have any further questions.

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Question

Hi!! I am a member of Our customer bought this product but it was in a transparent vial not in a dark vial. It was supposed to be in a dark vial according to your datasheet. What happened with it? Is it OK? I was so embarrassed. Our customer got a bad result with it so she has suspected the quality of this product due to storage condition. Please let me know if it doesn't affect the result or not. Bye.

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Answer

Thank you for your enquiry. This antibody does NOT need to be stored in the dark. It is not conjugated and is NOT sensitive to light. This was a mistake on the datasheet and has been amended. Should your customer continue to have problems I ask that they submit a technical questionaire so that we can determine what the nature of the problem is. I hope this information helps, please do not hesitate to contact us if you need any more advice or information.

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