Overview

  • Product name
    Anti-TetR antibody
  • Description
    Rabbit polyclonal to TetR
  • Host species
    Rabbit
  • Specificity
    This antibody detects Tet repressor protein. The Tet repressor protein regulates transcription of a family of tetracycline resistance determinants in Gram-negative bacteria. The Tet system is the most widely used regulatory system for conditional gene expression in eukaryotic cells. TetR can be used for that purpose in some organisms without further modifications. In mammals and in a large variety of other organisms, however, eukaryotic transcriptional activator or repressor domains are fused to TetR to turn it into an efficient regulator.
  • Tested applications
    Suitable for: WBmore details
  • Immunogen

    Recombinant fragment:

    AQKLGVEQPTLYWHVKNKR

    , corresponding to amino acids 31-49 of TetR

Properties

Applications

Our Abpromise guarantee covers the use of ab25845 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 24 kDa.

Target

  • Relevance
    TetR (Tet Repressor Protein) regulates transcription of a family of tetracycline resistance determinants in Gram-negative bacteria. The Tet system is the most widely used regulatory system for conditional gene expression in eukaryotic cells. TetR can be used for that purpose in some organisms without further modifications. In mammals and in a large variety of other organisms, however, eukaryotic transcriptional activator or repressor domains are fused to TetR to turn it into an efficient regulator.
  • Alternative names
    • pSC101 tetR antibody
    • Tet Repressor Protein antibody
    • Tetracycline Repressor Protein antibody
    • Tetracycline repressor protein class A from transposon 1721 antibody
    • Tetracycline repressor protein class B from transposon Tn10 antibody
    • Tetracycline repressor protein class C antibody
    • Tetracycline repressor protein class D antibody
    • Tetracycline repressor protein class E antibody
    see all

References

This product has been referenced in:
  • Whetstine CR  et al. Development of a single-plasmid-based regulatable gene expression system for Borrelia burgdorferi. Appl Environ Microbiol 75:6553-8 (2009). WB . Read more (PubMed: 19700541) »
See 1 Publication for this product

Customer reviews and Q&As

1-4 of 4 Abreviews or Q&A

Application
Western blot
Loading amount
30 µg
Gel Running Conditions
Reduced Denaturing (10% SDS PAGE)
Sample
Mouse Tissue lysate - whole (Left Ventricle)
Specification
Left Ventricle
Treatment
Doxycycline Chow for 3 days or 6 weeks
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Dr. Chris Tracy

Verified customer

Submitted Jan 02 2015

Answer

Thank you for taking the time to contact us. I am sorry to hear you have had difficulty obtaining satisfactory results from this antibody. I appreciate the time you have spent in the laboratory and understand your concerns. It is regrettable the results have not been successful. Before deciding how to proceed, our usual procedure is to investigate this particular case further for you, and also obtain some further information for our quality monitoring and records. In order to do this, I have enclosed a questionnaire below. I would appreciate if you could complete this. It will help you put the information we require together very easily.  We will happily offer technical support and in the event that a product is not functioning in the applications cited on the product data sheet (and the problem has been reported within 6 months of purchase), we will be pleased to provide a credit note, free of charge replacement or refund. Thank you for your time and cooperation. We look forward to receiving the completed questionnaire.     Order Details Antibody code: Problem Choose: Non-specific band Multiple bands No signal or weak signal High background Lot number Purchase order number or preferably Abcam order number: General Information Antibody storage conditions (temperature/reconstitution etc) Description of the problem (high background, wrong band size, more bands, no band etc.) Sample (Species/Cell extract/Nuclear extract/Purified protein/Recombinant protein etc.) Sample preparation (Buffer/Protease inhibitors/Heating sample etc.) Amount of protein loaded Electrophoresis/Gel conditions (Reducing or Non-reducing gel, % of the gel etc.) Transfer and blocking conditions (Buffer/time period, Blocking agent etc.) Primary Antibody (Manufacturer/Species/Diluent/Dilution/Incubation time, Wash step) Secondary Antibody (Manufacturer/Species/Diluent/Dilution/Incubation time, Wash step) Detection method (ECL, ECLPlus etc.) Positive and negative controls used (please specify) Optimization attempts (problem solving) How many times have you tried the Western? Have you run a "No Primary" control? Yes No Do you obtain the same results every time? Yes No e.g. are the background bands always in the same place? What steps have you altered? Additional Notes: Image: We would appreciate if you are able to provide an image (including molecular weight markers) which would help us to asses the results.  

Read More

Question
Answer

Thank you for your enquiry. Further to our telephone conversation this morning I would greatly appreciate it if you could provide me with further information of the protocol that you have been using by clicking on the link below and filling in our technical questionaire. This will better enable our technical team to determine the steps that you have taken to optimise this antibody. It would also be most informative if you could provide me with a representative blot and details of the batch number. https://www.abcam.com/index.html?section=western&pageconfig=technical&intAbID=25845&mode=questionaire I look forward to hearing from you.

Read More

Answer

I was informed that the immunogen for this antibody was amino acids 31-49 (AQKLGVEQPTLYWHVKNKR) of the Tet repressor protein (my apologies for the incorrect information on the datasheet stating it was the whole protein, this has been immediately corrected). This sequence is present in all know classes of TetR, so we would expect this antibody to recognize your tTS-TetR protein. I hope this information will help you,

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Sign up