Question (27535) | Anti-TetR antibody (ab25845)

Thank you for taking the time to contact us. I am sorry to hear you have had difficulty obtaining satisfactory results from this antibody. I appreciate the time you have spent in the laboratory and understand your concerns. It is regrettable the results have not been successful. Before deciding how to proceed, our usual procedure is to investigate this particular case further for you, and also obtain some further information for our quality monitoring and records. In order to do this, I have enclosed a questionnaire below. I would appreciate if you could complete this. It will help you put the information we require together very easily.  We will happily offer technical support and in the event that a product is not functioning in the applications cited on the product data sheet (and the problem has been reported within 6 months of purchase), we will be pleased to provide a credit note, free of charge replacement or refund. Thank you for your time and cooperation. We look forward to receiving the completed questionnaire.     Order Details Antibody code: Problem Choose: Non-specific band Multiple bands No signal or weak signal High background Lot number Purchase order number or preferably Abcam order number: General Information Antibody storage conditions (temperature/reconstitution etc) Description of the problem (high background, wrong band size, more bands, no band etc.) Sample (Species/Cell extract/Nuclear extract/Purified protein/Recombinant protein etc.) Sample preparation (Buffer/Protease inhibitors/Heating sample etc.) Amount of protein loaded Electrophoresis/Gel conditions (Reducing or Non-reducing gel, % of the gel etc.) Transfer and blocking conditions (Buffer/time period, Blocking agent etc.) Primary Antibody (Manufacturer/Species/Diluent/Dilution/Incubation time, Wash step) Secondary Antibody (Manufacturer/Species/Diluent/Dilution/Incubation time, Wash step) Detection method (ECL, ECLPlus etc.) Positive and negative controls used (please specify) Optimization attempts (problem solving) How many times have you tried the Western? Have you run a "No Primary" control? Yes No Do you obtain the same results every time? Yes No e.g. are the background bands always in the same place? What steps have you altered? Additional Notes: Image: We would appreciate if you are able to provide an image (including molecular weight markers) which would help us to asses the results.