Recombinant
RabMAb

Recombinant Anti-Tetranectin antibody [EPR3067] (ab108999)

Overview

  • Product name

    Anti-Tetranectin antibody [EPR3067]
    See all Tetranectin primary antibodies
  • Description

    Rabbit monoclonal [EPR3067] to Tetranectin
  • Host species

    Rabbit
  • Tested applications

    Suitable for: ICC/IF, IHC-Fr, WB, IP, IHC-Pmore details
    Unsuitable for: Flow Cyt
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human Tetranectin aa 150-250. The exact sequence is proprietary.

  • Positive control

    • Human fetal kidney and Human plasma lysates; Human kidney tissue.
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab108999 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/250 - 1/500.
IHC-Fr Use at an assay dependent concentration.
WB 1/1000 - 1/10000. Detects a band of approximately 22 kDa (predicted molecular weight: 23 kDa).
IP 1/10 - 1/100.
IHC-P 1/500 - 1/1000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
  • Application notes
    Is unsuitable for Flow Cyt.
  • Target

    • Function

      Tetranectin binds to plasminogen and to isolated kringle 4. May be involved in the packaging of molecules destined for exocytosis.
    • Tissue specificity

      Found in plasma.
    • Sequence similarities

      Contains 1 C-type lectin domain.
    • Cellular localization

      Secreted.
    • Information by UniProt
    • Database links

    • Alternative names

      • C type lectin domain family 3 member B antibody
      • C-type lectin domain family 3 member B antibody
      • Clec3b antibody
      • Plasminogen kringle 4 binding protein antibody
      • Plasminogen kringle 4-binding protein antibody
      • TETN_HUMAN antibody
      • Tetranectin (plasminogen binding protein) antibody
      • Tetranectin antibody
      • TN antibody
      • TNA antibody
      see all

    Images

    • All lanes : Anti-Tetranectin antibody [EPR3067] (ab108999) at 1/1000 dilution

      Lane 1 : Human fetal kidney lysate
      Lane 2 : Human plasma lysate

      Lysates/proteins at 10 µg per lane.

      Predicted band size: 23 kDa

    • ab108999 at 1/500 dilution staining Tetranectin in Human kidney by Immunohistochemistry, Paraffin-embedded tissue.

      Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

    References

    This product has been referenced in:

    • Liu J  et al. CLEC3B is downregulated and inhibits proliferation in clear cell renal cell carcinoma. Oncol Rep 40:2023-2035 (2018). Read more (PubMed: 30066941) »
    See 1 Publication for this product

    Customer reviews and Q&As

    1-7 of 7 Abreviews or Q&A

    Answer

    Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products.
    I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has caused. As requested, I have issued a free of charge replacement with the order number 1116275.
    To check the status of the order please contact our Customer Service team and reference this number.
    Please note that this free of charge replacement vial is also covered by our Abpromise guarantee. Should you still be experiencing difficulties, or if you have any further questions, please do not hesitate to let us know.
    I wish you the best of luck with your research.

    Read More

    Question
    Answer

    Thank you for your email.
    I had chat with my colleague who is responsible for FedEx shipments. She has confirmed that Abcam or FedEx do not have any control on shipments, that are delayed at Customs so the customer might need to contact the customs in their country for updates.
    I am happy to send you this antibody again at no extra charge; normally it takes 2 days to deliver howver if it gets stuck again then unfortunatley we can not do any thing. We will try to leave as much information as we can on the dilvery note for customs clearence however you may need to keep track of your order also.
    Could you confirm if you would like to recieve the same antibody. I will then send you the details with order number?

    Read More

    Answer

    Thank you very much for your cooperation in this case. I was checking my previous cases and I came across this one.
    Could you confirm if you have received an update from Abcam customer service team? I apologize for the delay as this email got missed.
    Looking forward to hearing from you soon.

    Read More

    Answer

    Thank you for your email. I am sorry to hear that FedEx refused to compensate for this.
    I am now working with our customer service team to check the problem created by customs because it was never happened before.
    I will get back you with the updates soon.

    Read More

    Answer

    Thank you for contacting us. I am very sorry to hear about the delay.
    As discussed on phone please ask Fedex for compensation.
    I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

    Read More
    Application
    Immunohistochemistry (Frozen sections)
    Sample
    Human Tissue sections (Kidney)
    Specification
    Kidney
    Fixative
    Acetone
    Permeabilization
    No
    Blocking step
    Serum as blocking agent for 20 minute(s) · Concentration: 10.0% · Temperature: 21°C

    Abcam user community

    Verified customer

    Submitted Feb 17 2012

    Application
    Western blot
    Sample
    Human Serum (Normal control human serum)
    Loading amount
    10 µg
    Specification
    Normal control human serum
    Gel Running Conditions
    Reduced Denaturing (4-15% Gradient Gel)
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C

    Abcam user community

    Verified customer

    Submitted Jul 12 2011

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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