RecombinantRabMAb

Recombinant Anti-Tetranectin antibody [EPR3067] (ab108999)

Reviews (2)Q&A (5)References (3)
Western blot - Anti-Tetranectin antibody [EPR3067] (ab108999)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tetranectin antibody [EPR3067] (ab108999)
  • Anti-Tetranectin antibody [EPR3067] (ab108999)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR3067] to Tetranectin
  • Suitable for: WB, IHC-P
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-Tetranectin antibody [EPR3067]
    See all Tetranectin primary antibodies

  • Description

    Rabbit monoclonal [EPR3067] to Tetranectin

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IHC-Pmore details
    Unsuitable for: Flow Cyt

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide within Human Tetranectin aa 150-250. The exact sequence is proprietary.

  • Positive control

    • Human fetal kidney and Human plasma lysates; Human kidney tissue.

  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

Applications

Our Abpromise guarantee covers the use of ab108999 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 22 kDa (predicted molecular weight: 23 kDa).
IHC-P 1/500 - 1/1000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
  • Application notes
    Is unsuitable for Flow Cyt.

    • Target

    • Function

      Tetranectin binds to plasminogen and to isolated kringle 4. May be involved in the packaging of molecules destined for exocytosis.

    • Tissue specificity

      Found in plasma.

    • Sequence similarities

      Contains 1 C-type lectin domain.

    • Cellular localization

      Secreted.
    • Information by UniProt

    • Database links

      • Alternative names

        • C type lectin domain family 3 member B antibody
        • C-type lectin domain family 3 member B antibody
        • Clec3b antibody
        • Plasminogen kringle 4 binding protein antibody
        • Plasminogen kringle 4-binding protein antibody
        • TETN_HUMAN antibody
        • Tetranectin (plasminogen binding protein) antibody
        • Tetranectin antibody
        • TN antibody
        • TNA antibody
        see all

      Images

      • Western blot - Anti-Tetranectin antibody [EPR3067] (ab108999)
        Western blot - Anti-Tetranectin antibody [EPR3067] (ab108999)
        All lanes : Anti-Tetranectin antibody [EPR3067] (ab108999) at 1/1000 dilution

        Lane 1 : Human fetal kidney lysate
        Lane 2 : Human plasma lysate

        Lysates/proteins at 10 µg per lane.

        Predicted band size: 23 kDa

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tetranectin antibody [EPR3067] (ab108999)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tetranectin antibody [EPR3067] (ab108999)

        ab108999 at 1/500 dilution staining Tetranectin in Human kidney by Immunohistochemistry, Paraffin-embedded tissue.

        Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

      • Anti-Tetranectin antibody [EPR3067] (ab108999)
        Anti-Tetranectin antibody [EPR3067] (ab108999)

      References (3)

      Publishing research using ab108999? Please let us know so that we can cite the reference in this datasheet.

      ab108999 has been referenced in 3 publications.

      • Sun J  et al. CLEC3B as a potential diagnostic and prognostic biomarker in lung cancer and association with the immune microenvironment. Cancer Cell Int 20:106 (2020). PubMed: 32265595
      • Zhu HF  et al. Cancer-associated fibroblasts promote colorectal cancer progression by secreting CLEC3B. Cancer Biol Ther 20:967-978 (2019). PubMed: 30894065
      • Liu J  et al. CLEC3B is downregulated and inhibits proliferation in clear cell renal cell carcinoma. Oncol Rep 40:2023-2035 (2018). PubMed: 30066941

      Customer reviews and Q&As

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      1-5 of 5 Q&A

      Question

      I understand the issues regarding clearing of customs, the circumstances were unfortunate and in my experience rare. I would very much like to receive the same antibody. It this could be sent out on Monday next week, this should help to ensure that the package does not sit at customs over the weekend. I would also like the antibody to be sent directly to my collaborators, minimizing handling time. Please send to:

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      Answer

      Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products.
      I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has caused. As requested, I have issued a free of charge replacement with the order number 1116275.
      To check the status of the order please contact our Customer Service team and reference this number.
      Please note that this free of charge replacement vial is also covered by our Abpromise guarantee. Should you still be experiencing difficulties, or if you have any further questions, please do not hesitate to let us know.
      I wish you the best of luck with your research.

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      Question

      No, I have not received an update from Abcam, but I would very much like one.

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      Answer

      Thank you for your email.
      I had chat with my colleague who is responsible for FedEx shipments. She has confirmed that Abcam or FedEx do not have any control on shipments, that are delayed at Customs so the customer might need to contact the customs in their country for updates.
      I am happy to send you this antibody again at no extra charge; normally it takes 2 days to deliver howver if it gets stuck again then unfortunatley we can not do any thing. We will try to leave as much information as we can on the dilvery note for customs clearence however you may need to keep track of your order also.
      Could you confirm if you would like to recieve the same antibody. I will then send you the details with order number?

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      Question

      I just spoke with FedEx. Due to the fact that the product was delayed in customs, FedEx will not compensate. This is very unfortunate. I need this antibody as a final control for a submitted publication. However, I am not keen on spending more money on a antibody that I have already purchased. How do you suggest I proceed in this matter.
      Regards,

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      Answer

      Thank you very much for your cooperation in this case. I was checking my previous cases and I came across this one.
      Could you confirm if you have received an update from Abcam customer service team? I apologize for the delay as this email got missed.
      Looking forward to hearing from you soon.

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      Question

      I just spoke with FedEx. Due to the fact that the product was delayed in customs, FedEx will not compensate. This is very unfortunate. I need this antibody as a final control for a submitted publication. However, I am not keen on spending more money on a antibody that I have already purchased. How do you suggest I proceed in this matter.
      Regards,

      Read More
      Answer

      Thank you for your email. I am sorry to hear that FedEx refused to compensate for this.
      I am now working with our customer service team to check the problem created by customs because it was never happened before.
      I will get back you with the updates soon.

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      Question

      Antibody received after 10 days. Stuck with Fedex.
      At room temperature when received.
      Giving multiple bands in WB and high background in IHC

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      Answer

      Thank you for contacting us. I am very sorry to hear about the delay.
      As discussed on phone please ask Fedex for compensation.
      I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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      Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
      For licensing inquiries, please contact partnerships@abcam.com