Application
Western blot
Sample
Human Serum (Normal control human serum)
Loading amount
10 µg
Specification
Normal control human serum
Gel Running Conditions
Reduced Denaturing (4-15% Gradient Gel)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
Other product details
Dilution
1/1000
Incubation time
1 hour(s) and 0 minute(s) · Temperature: 21°C · Diluent: 10mM sodium phosphate, pH 7.4, 150mM NaC
Secondary antibody
Name
Non-Abcam antibody was used: Dako anti-rabbit HRP conjugated antibody
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/5000
Detection
Detection method
ECL
Exposure
2 minute(s) and 0 second(s)
Bands
Specific: 22.5 kDa
Negative control
Primary antibody ommitted and no bands observed, so the band is not due to a secondary antibody cross reaction. Also band was not observed when using an irrelevant primary antibody control with the same secondary antibody and detection system.
Abcam user community
Verified customer
Submitted Jul 12 2011