Product nameAnti-Tetraspanin-33 antibody
See all Tetraspanin-33 primary antibodies
DescriptionRabbit polyclonal to Tetraspanin-33
Tested applicationsSuitable for: WB, IHC-Pmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Cow, Zebrafish
Synthetic peptide corresponding to Human Tetraspanin-33 aa 250 to the C-terminus (C terminal) conjugated to keyhole limpet haemocyanin.
(Peptide available as
- This antibody gave a positive signal in Human Spleen Tissue Lysate.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
Concentration information loading...
PurityImmunogen affinity purified
Immunizing Peptide (Blocking)
Our Abpromise guarantee covers the use of ab79130 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 1 mg/ml. Detects a band of approximately 32 kDa (predicted molecular weight: 32 kDa).|
|IHC-P||Use a concentration of 5 µg/ml.|
FunctionPlays an important role in normal erythropoiesis. It has a role in the differentiation of erythroid progenitors.
Tissue specificityPredominantly expressed in erythroblasts.
Sequence similaritiesBelongs to the tetraspanin (TM4SF) family.
- Information by UniProt
- 1300010A20Rik antibody
- AI035228 antibody
- hPen antibody
Anti-Tetraspanin-33 antibody (ab79130) at 1 µg/ml + Human spleen tissue lysate - total protein (ab29699) at 10 µg
Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 32 kDa
Observed band size: 30 kDa why is the actual band size different from the predicted?
Exposure time: 5 minutes
IHC image of ab79130 staining in Hodgekin's Lymphoma formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab79130, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.