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    tetraspanin-33-antibody-ab79130.pdf

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Anti-Tetraspanin-33 antibody (ab79130)

  • Datasheet
  • SDS
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Western blot - Anti-Tetraspanin-33 antibody (ab79130)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tetraspanin-33 antibody (ab79130)

Key features and details

  • Rabbit polyclonal to Tetraspanin-33
  • Suitable for: WB, IHC-P
  • Reacts with: Human
  • Isotype: IgG

You may also be interested in

Secondary
Product image
Goat Anti-Rabbit IgG H&L (HRP) (ab205718)

View more associated products

Overview

  • Product name

    Anti-Tetraspanin-33 antibody
    See all Tetraspanin-33 primary antibodies
  • Description

    Rabbit polyclonal to Tetraspanin-33
  • Host species

    Rabbit
  • Tested Applications & Species

    Application Species
    WB
    Human
    See all applications and species data
  • Immunogen

    Synthetic peptide corresponding to Human Tetraspanin-33 aa 250 to the C-terminus (C terminal) conjugated to keyhole limpet haemocyanin.
    (Peptide available as ab87705)

  • Positive control

    • This antibody gave a positive signal in Human Spleen Tissue Lysate.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituent: PBS

    Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Cell Biology
    • Other Antibodies
    • Other Antibodies

Associated products

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Immunizing Peptide (Blocking)

    • Human Tetraspanin-33 peptide (ab87705)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab79130 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

Application Species
WB
Human
All applications
Mouse
Cow
Zebrafish
Application Abreviews Notes
WB
Use a concentration of 1 mg/ml. Detects a band of approximately 32 kDa (predicted molecular weight: 32 kDa).
IHC-P
Use a concentration of 5 µg/ml.
Notes
WB
Use a concentration of 1 mg/ml. Detects a band of approximately 32 kDa (predicted molecular weight: 32 kDa).
IHC-P
Use a concentration of 5 µg/ml.

Target

  • Function

    Plays an important role in normal erythropoiesis. It has a role in the differentiation of erythroid progenitors.
  • Tissue specificity

    Predominantly expressed in erythroblasts.
  • Sequence similarities

    Belongs to the tetraspanin (TM4SF) family.
  • Cellular localization

    Membrane.
  • Target information above from: UniProt accession Q86UF1 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 340348 Human
    • Entrez Gene: 232670 Mouse
    • Entrez Gene: 436890 Zebrafish
    • Omim: 610120 Human
    • SwissProt: Q3SYV5 Cow
    • SwissProt: Q86UF1 Human
    • SwissProt: Q8R3S2 Mouse
    • SwissProt: Q5RH71 Zebrafish
    • Unigene: 27267 Human
    • Unigene: 225289 Mouse
    • Unigene: 88692 Zebrafish
    see all
  • Alternative names

    • 1300010A20Rik antibody
    • AI035228 antibody
    • hPen antibody
    • MGC30714 antibody
    • MGC50844 antibody
    • PEN antibody
    • Penumbra antibody
    • Proerythroblast new membrane antibody
    • RGD1560915 antibody
    • Tetraspanin-33 antibody
    • TSN33 antibody
    • TSN33_HUMAN antibody
    • Tspan-33 antibody
    • TSPAN33 antibody
    • Tspan33 tetraspanin 33 antibody
    see all

Images

  • Western blot - Anti-Tetraspanin-33 antibody (ab79130)
    Western blot - Anti-Tetraspanin-33 antibody (ab79130)
    Anti-Tetraspanin-33 antibody (ab79130) at 1 µg/ml + Human spleen tissue lysate - total protein (ab29699) at 10 µg

    Secondary
    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 32 kDa
    Observed band size: 30 kDa why is the actual band size different from the predicted?


    Exposure time: 5 minutes
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tetraspanin-33 antibody (ab79130)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tetraspanin-33 antibody (ab79130)

    IHC image of ab79130 staining in Hodgkin's Lymphoma formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab79130, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

Protocols

  • Western blot protocols
  • Immunohistochemistry protocols

Click here to view the general protocols

Datasheets and documents

  • SDS download

  • Datasheet download

    Download

References (1)

Publishing research using ab79130? Please let us know so that we can cite the reference in this datasheet.

ab79130 has been referenced in 1 publication.

  • Luu VP  et al. TSPAN33 is a novel marker of activated and malignant B cells. Clin Immunol 149:388-99 (2013). Human . PubMed: 24211713

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