For the best experience on the Abcam website please upgrade to a modern browser such as Google Chrome

Hello. We're improving abcam.com and we'd welcome your feedback.

Hello. We're improving abcam.com and we'd welcome your feedback.

Infomation icon

We haven't added this to the BETA yet

New BETA website

New BETA website

Hello. We're improving abcam.com and we'd welcome your feedback.

Take a look at our BETA site and see what we’ve done so far.

Switch on our new BETA site

Now available

Search and browse selected products

  • A selection of primary antibodies

Purchase these through your usual distributor

In the coming months

  • Additional product types
  • Supporting content
  • Sign in to your account
  • Purchase online
United States
Your country/region is currently set to:

If incorrect, please enter your country/region into the box below, to view site information related to your country/region.

Call (888) 77-ABCAM (22226) or contact us
Need help? Contact us

  • My account
  • Sign out
Sign in or Register with us

Welcome

Sign in or

Don't have an account?

Register with us
My basket
Quick order
Abcam homepage

  • Research Products
    By product type
    Primary antibodies
    Secondary antibodies
    ELISA and Matched Antibody Pair Kits
    Cell and tissue imaging tools
    Cellular and biochemical assays
    Proteins and Peptides
    By product type
    Proteomics tools
    Agonists, activators, antagonists and inhibitors
    Cell lines and Lysates
    Multiplex miRNA assays
    Multiplex Assays
    By research area
    Cancer
    Cardiovascular
    Cell Biology
    Epigenetics
    Metabolism
    Developmental Biology
    By research area
    Immunology
    Microbiology
    Neuroscience
    Signal Transduction
    Stem Cells
  • Customized Products & Partnerships
    Customized Products & Partnerships

    Customized products and commercial partnerships to accelerate your diagnostic and therapeutic programs.

    Customized products

    Partner with us

  • Support
    Support hub

    Access advice and support for any research roadblock

    View support hub

    Protocols

    Your experiments laid out step by step

    View protocols

  • Events
    • Conference calendar
    • Cancer
    • Cardiovascular
    • Epigenetics & Nuclear signaling
    • Immunology
    • Neuroscience
    • Stem cells
    • Tradeshows
    • Scientific webinars
    Keep up to date with the latest events

    Full event breakdown with abstracts, speakers, registration and more

    View global event calendar

  • Pathways
    Cell signalling pathways

    View all pathways

    View all interactive pathways

Explore the power of knock-out cell lines for your research

  1. Link

    tetrodotoxin-na-channel-blocker-ab120054.pdf

  1. Send me a copy of this email
    I agree to the terms and conditions.
Biochemicals Chemical Type Biochemicals
Share by email

Tetrodotoxin, Na+ channel blocker (ab120054)

  • Datasheet
  • SDS
  • COA
Submit a review Q&A (6)References (222)

Product price, shipping and contact information

Currently unavailable

Sorry, we can't display this right now.

Please contact us to place your order, or try again later.

 

Loading size & price…

 

Shipping and order information

Shipping info

Promotion Information

Abpromise

Guaranteed product quality, expert customer support.

Find out more.

Chemical Structure - Tetrodotoxin, Na<sup>+</sup> channel blocker (ab120054)
  • Functional Studies - Tetrodotoxin, Na<sup>+</sup> channel blocker (ab120054)
  • Functional Studies - Tetrodotoxin, Na<sup>+</sup> channel blocker (ab120054)

Key features and details

  • Na+ channel blocker
  • CAS Number: 4368-28-9
  • Soluble in pH 4.8 citrate buffer to 100 mM
  • Form / State: Solid
  • Source: Fugu

You may also be interested in

Biochemical
Product image
D-AP5, NMDA glutamate site antagonist (ab120003)
Cells
Product image
ioGlutamatergic Neurons – Human iPSC-Derived Glutamatergic Neurons (ab259259)

View more associated products

Overview

  • Product name

    Tetrodotoxin, Na+ channel blocker
  • Description

    Na+ channel blocker
  • Alternative names

    • TTX
  • Biological description

    Potent, selective and reversible, use-dependent inhibitor of voltage-dependent Na+ channels.

  • General notes

    Item is subject to Export Control Regulations and Licensing requirements. Please contact Customer Service for more information and lead times.

  • CAS Number

    4368-28-9
  • Chemical structure

    Chemical Structure

Properties

  • Chemical name

    Octahydro-12-(hydroxymethyl)-2-imino-5,9:7,10a-dimethano-10aH-[1,3]dioxocino[6,5-d] pyrimidine-4,7,10,11,12-pentol
  • Molecular weight

    319.27
  • Molecular formula

    C11H17N3O8
  • PubChem identifier

    6324668
  • Storage instructions

    Store at -20°C. Store under desiccating conditions. The product can be stored for up to 12 months.
  • Solubility overview

    Soluble in pH 4.8 citrate buffer to 100 mM
  • Handling

    Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20°C. Generally, these will be useable for up to one month. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour.

    Toxic, refer to SDS for further information.

    Need more advice on solubility, usage and handling? Please visit our frequently asked questions (FAQ) page for more details.

  • SMILES

    O[C@H]3C14NC(=N)N[C@H](O)[C@@H]4C2O[C@]3(O)O[C@H](C1O)C2(O)CO
  • Source

    Fugu

  • Research areas

    • Biochemicals
    • Chemical Type
    • Biochemicals
    • Biochemicals
    • Chemical Type
    • Toxin
    • Biochemicals
    • Pharmacology
    • Ion Channels
    • Sodium
    • Blockers
    • Biochemicals
    • Research Area
    • Diabetes
    • Sodium
    • Blockers
    • Biochemicals
    • Research Area
    • Heart disease
    • Sodium
    • Blockers
    • Biochemicals
    • Research Area
    • Hypertension
    • Sodium
    • Blockers
    • Biochemicals
    • Research Area
    • Obesity
    • Sodium
    • Blockers
    • Biochemicals
    • Research Area
    • Pain & inflammation
    • Sodium
    • Blockers
    • Biochemicals
    • Research Area
    • Respiratory disease
    • Sodium
    • Blockers
    • Biochemicals
    • Research Area
    • Stroke
    • Sodium
    • Blockers

Associated products

  • Related Products

    • ioGlutamatergic Neurons – Human iPSC-Derived Glutamatergic Neurons (ab259259)

Images

  • Chemical Structure - Tetrodotoxin, Na<sup>+</sup> channel blocker (ab120054)
    Chemical Structure - Tetrodotoxin, Na+ channel blocker (ab120054)
    2D chemical structure image of ab120054, Tetrodotoxin, Na+ channel blocker
  • Functional Studies - Tetrodotoxin, Na<sup>+</sup> channel blocker (ab120054)
    Functional Studies - Tetrodotoxin, Na+ channel blocker (ab120054)Image from Gómez-Gonzalo M et al., PLoS Biol. 2010;8(4):e1000352. Fig 4(E).; doi: 10.1371/journal.pbio.1000352. Reproduced under the Creative Commons license http://creativecommons.org/licenses/by/4.0/
    TTX blocked the ictal discharge in both field A and B neurons, but not the direct response of field A neurons to a double NMDA pulse (black arrowheads).
  • Functional Studies - Tetrodotoxin, Na<sup>+</sup> channel blocker (ab120054)
    Functional Studies - Tetrodotoxin, Na+ channel blocker (ab120054)Image from Riebe I et al., BMC Neuroscience. 2012;13:14. Fig 1.; doi: 10.1186/1471-2202-13-14. Reproduced under the Creative Commons license http://creativecommons.org/licenses/by/2.0
    Multiple connections between CA3 and CA1 pyramidal cells and GABAergic cells and CA1 pyramidal cells in young adult rats. (A1) A typical recording at -80 mV from a pyramidal cell, upper trace shows AMPA sEPSCs under control conditions and lower trace shows mEPSCs after wash-in of TTX. (A2) TTX reduces the AMPA EPSC amplitude by 5.9 ± 0.8 pA (P = 0.004, n = 8, two-tailed paired t-test). (A3) TTX reduces AMPA EPSC frequency by 1.5 ± 0.4 Hz (P < 0.001, n = 8, one-tailed paired t-test). (A4) The average multiplicity was 1.7 ± 0.1 (P < 0.001, n = 8, one-tailed one-sample t-test with test value = 1). (B1) A typical recording at 0 mV from a pyramidal cell, upper trace shows GABA sIPSCs under control conditions and lower trace shows mIPSCs after wash-in of TTX. (B2) TTX reduces the GABA IPSC amplitude by 8.4 ± 2.7 pA (P = 0.03, n = 6, two-tailed paired t-test). (B3) TTX reduces GABA IPSC frequency by 3.2 ± 0.8 Hz (P = 0.005, n = 6, one-tailed paired t-test). (B4) The average multiplicity was 2

Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

Datasheets and documents

  • SDS download

  • Datasheet download

    Download
  • COA

References (222)

Publishing research using ab120054? Please let us know so that we can cite the reference in this datasheet.

ab120054 has been referenced in 222 publications.

  • Wei XP  et al. A novel reticular node in the brainstem synchronizes neonatal mouse crying with breathing. Neuron 110:644-657.e6 (2022). PubMed: 34998469
  • Biegler MT  et al. Induction of an immortalized songbird cell line allows for gene characterization and knockout by CRISPR-Cas9. Sci Rep 12:4369 (2022). PubMed: 35288582
  • Vaden RJ  et al. Parvalbumin interneurons provide spillover to newborn and mature dentate granule cells. Elife 9:N/A (2020). PubMed: 32602839
  • Zabolocki M  et al. BrainPhys neuronal medium optimized for imaging and optogenetics in vitro. Nat Commun 11:5550 (2020). PubMed: 33144563
  • Wang X  et al. Restoring tripartite glutamatergic synapses: A potential therapy for mood and cognitive deficits in Gulf War illness. Neurobiol Stress 13:100240 (2020). PubMed: 33344696
View all Publications for this product

Customer reviews and Q&As

Show All Reviews Q&A
Submit a review Submit a question

1-6 of 6 Abreviews or Q&A

Question

What molarity citrate buffer should be used for reconstitution?

Read More

Abcam community

Verified customer

Asked on Nov 22 2013

Answer

The specific molarity of the citrate buffer used to solubilize TTX (ab120054) is not as significant as the pH of 4.8. You can essentially use the molarity of citrate buffer that will be applicable with your specific study. As a general guideline for a range that can be used you can use 20 mM Citrate buffer. I also found the following paper which may be helpful, which used 1.5 mM citrate buffer, pH 4.8 to dissolve TTX to a concentration of 300 or 500 uM.

http://www.jneurosci.org/content/17/11/4359.full.pdf

Read More

Kevin Hanson

Abcam Scientific Support

Answered on Nov 22 2013

Question

What is the molecular weight of tetrodotoxin citrate? The datasheets for this product, ab120055, and tetrodotoxin, ab120054, give the same molecular weight (319.27) and same molecular formula (C11H17N3O8).

Read More

Abcam community

Verified customer

Asked on Jun 14 2013

Answer

The water-soluble TTX citrate ab120055 is not a citrate salt, it is simply TTX that has been dissolved in a citrate solution, and then lyophilized. So, it has the same molecular weight and formula as ab120054, which is soluble in pH 4.8 citrate buffer to 100 mM.

Read More

Tom Ruyle

Abcam Scientific Support

Answered on Jun 14 2013

Question

I am establishing an assay using Tetrodotoxin (ab120054) as an antagonist for the Sodium channels. I ve have seen a decrease in the inhibition of the depolarization between the different measurements.
The TTX Stock was dissolved in Citrate Buffer pH 4.8. This Stock is further diluted in Electrophysiology Buffer. This solution containing TTX was 6h at room temperature, rsp 6h between The first and the last measurement. Do you have experience with the stability of solubilized TTX?

Thank you in advance for your help.

Please reply on this email address (gmail), as we have a technical problem with the ZHAW email.

Best wishes

Read More

Abcam community

Verified customer

Asked on Feb 21 2013

Answer

Thank you for contacting us.

The following information is published on the datasheet;

Providing storage is as stated on the product vial and the vial is kept tightly sealed, the product can be stored for up to 6 months.

Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20°C. Generally, these will be useable for up to one month. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour.

In short, the stability of product should not be affected in a lapse of 6 hours.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Read More

Abcam Scientific Support

Answered on Feb 21 2013

Question

I use TTX quite a lot and know it's highly toxic. Looking at the SDS however it's classed as Category 2 for acute inhalation toxicity which seems a bit surprising. It would be expected that this product would be at least as toxic by inhalation as by oral or dermal routes given that it's partially broken in the stomach.

Read More

Abcam community

Verified customer

Asked on Jan 17 2013

Answer

Despite extensive checking, including the RTECS database, we can find no toxicity data for inhalation (or skin contact) for TTX. In theory, we are not obliged to include any classification for these routes of entry if no data can be found. Obviously, we think it prudent to include the inhalation and skin contact hazards according to our judgement to make sure we give an indication that we believe TTX to be extremely toxic by all entry routes and not just by ingestion. It is worth noting that some suppliers only include the warning: ‘fatal by ingestion, might be irritating to the respiratory tract, might be irritating to the skin’.

If we were to classify TTX as category 1 by inhalation that would preclude the shipment of this material by air as the material would fall outside of ‘Dangerous Goods in Excepted Quantities’. So we have taken the following pragmatic approach:

The oral LD50 for a mouse is 334ug/kg and if this scales directly to a human, then 25mg would kill a 75kg man. If the inhalation route of entry has a similar level of toxicity (not unreasonable since both inhalation and ingestion require absorption through a moist membrane) then we do not sell TTX in sufficient quantities to prove fatal. Furthermore, the inhalation mode of entry requires (for a solid) the material to be in the form of an aerosol (dust or mist) and we feel that supplied as a lyophilised solid (generally stuck to the inside of the vial) then our product, a reasonably high molecular weight (319), low volatility solid is unlikely to be encountered in this form when handled using Good Laboratory Practices.

We believe that our SDS accurately conveys the hazard potential of this product and as it is intended solely for use in a laboratory, by trained personnel the likelihood of exposure to a fatal level is extremely remote.

Read More

Abcam Scientific Support

Answered on Jan 17 2013

Question

Customer kindly contacted us regarding the difference between ab1202054 (TTX) and ab120055 (TTX-Citrate).

Read More

Abcam community

Verified customer

Asked on Jan 14 2013

Answer


I contacted the laboratory regarding the difference between ab120054 and ab120055, and they responded that the only difference between these two compounds is that TTX citrate (ab120055) is the water soluble version, which accounts for the difference in price.

Read More

Abcam Scientific Support

Answered on Jan 14 2013

Question

Hello,

Please not Purity Requirement : >98% (Preferably HPLC) at least TLC. Please Send Certificate of Analysis.

Thank you

Read More

Abcam community

Verified customer

Asked on Feb 27 2012

Answer

Thank you for contacting us.


SL 327 (ab120082) and Kainic acid (ab120100) are both produced at >98% purity as determined by HPLC. I am checking which lots of these products have been assigned to you and then I will send the certificate of analysis for these lots.


The TTX is produced at >96% purity as determined by HPLC but we have stocks of this which is of >98% purity and I will again make sure that these stocks are being allocated to you and send the certificate of analysis.


In the meantime if you have any further questions please do not hesitate to ask.

Read More

Abcam Scientific Support

Answered on Feb 27 2012

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES, NOT FOR USE IN HUMANS"
For licensing inquiries, please contact partnerships@abcam.com

Get resources and offers direct to your inbox Sign up
A-Z by research area
  • Cancer
  • Cardiovascular
  • Cell biology
  • Developmental biology
  • Epigenetics & Nuclear signaling
  • Immunology
  • Metabolism
  • Microbiology
  • Neuroscience
  • Signal transduction
  • Stem cells
A-Z by product type
  • Primary antibodies
  • Secondary antibodies
  • Biochemicals
  • Isotype controls
  • Flow cytometry multi-color selector
  • Kits
  • Loading controls
  • Lysates
  • Peptides
  • Proteins
  • Slides
  • Tags and cell markers
  • Tools & Reagents
Help & support
  • Support
  • Make an Inquiry
  • Protocols & troubleshooting
  • Placing an order
  • RabMAb products
  • Biochemical product FAQs
  • Training
  • Browse by Target
Company
  • Corporate site
  • Investor relations
  • Company news
  • Careers
  • About us
  • Blog
Events
  • Tradeshows
  • Conferences
International websites
  • abcam.cn
  • abcam.co.jp

Join with us

  • LinkedIn
  • facebook
  • Twitter
  • YouTube
  • Terms of sale
  • Website terms of use
  • Cookie policy
  • Privacy policy
  • Legal
  • Modern slavery statement
© 1998-2022 Abcam plc. All rights reserved.